Klenow fragment is formed by loss of fragment having which activity:
5'- 3' polymerase
3'- 5' exonuclease
5'- 3' exonuclease
3'- 5' polymerase
Klenow fragment Large fragment produced by Subtilisin mediated proteolytic cleavage of E.Coli DNA polymerase I. Proteolysis removes the 5' -->3' exonuclease activity from N-terminal. Klenow fragment - Functions : Remove 3' overhang Fills 5' overhangs Synthesis of double-stranded DNA from single-stranded templates Preparation of radioactive DNA probes Was used in PCR