Biopsy from an eight-year-old child with leg swelling showed smallround blue tumor cells consistent with diagnosis of Ewing's sarcoma. What will be the best method to detect translocation t(ll;22) in this malignancy?
Conventional karyotyping
Next generation sequencing
FISH
PCR
Ans. C. FISHFISH is a molecular cytogenetic technique that uses fluorescent probes that bind to only those pas of the chromosome with a high degree of sequence complementarity.