In a suspected patient of dengue, all of these are acceptable investigations at day 3 of presentation except:
Viral culture and isolation in C6/36 cell line
ELISA for antibody against dengue virus
NS I antigen detection
#NAME?
Ans: B. ELISA for antibody against dengue virus(Ref Ananthanarayan 10/e p529, 8/e p519: Harrison 19/c 1)1319).ELISA in antibody detection against Dengue virus:Yields diagnostic results after 5 days (acute infection phase) for antibody detection against dengue virus.Other diagnosis methods:For mosquito inoculation/mosquito cell culture - Virus readily isolated from blood in acute phase.Laboratory tests for Dengue:Virus isolation within six days: Serum, plasma, autopsy tissueViral nucleic acid detection (-PCR assay).Diagnostic MethodTime to ResultsSpecimenTime of collection after onset of symptomsViral isolation & serotype identification1-2 weeksWhole blood, serum, tissues1-5 daysNucleic acid detection1 or 2 daysTissues, whole blood, serum, plasma1-5 daysdegAntigen detection1 daySerum1-6 daysdeg >1 dayTissue for immunochemistryNAIgM ELISA1-2 daysSerum, plasma, whole blood After 5 daysa