After a point mutation, glutamic acid is replaced by valine, which leads to formation of sickle cell hemoglobin. The mobility of HbS as compared with normal hemoglobin on gel electrophoresis will be:
Decreased
Increased
Dependent on HbS concentration
Unchanged.
HbS mobility on gel electrophoresis:Decreased mobility - Compared to normal hemoglobin.Electrophoresis of hemoglobin:Obtained from lysed red blood cells.Used in sickle cell trait & disease diagnosis.Sequence of Movement: HbA2 < HbC < HhS < HbF < HbA