Got it! So, we have 85482 and 64131, and we'll subtract the latter from the former.

Step 1: We'll start by subtracting the digit 1 and the borrow 0 from 2 in column 1 and get 1.
1 is the first digit of our result.

Step 2: We'll start by subtracting the digit 3 and the borrow 0 from 8 in column 2 and get 5.
5 is the next digit of our result.

Step 3: We'll start by subtracting the digit 1 and the borrow 0 from 4 in column 3 and get 3.
3 is the next digit of our result.

Step 4: We'll start by subtracting the digit 4 and the borrow 0 from 5 in column 4 and get 1.
1 is the next digit of our result.

Step 5: We'll start by subtracting the digit 6 and the borrow 0 from 8 in column 5 and get 2.
2 is the next digit of our result.

So, 85482 - 64131 = 21351.