Effect of heparin and ethanol on the activity of lactate dehydrogenase in mitochondrial and cytoplasmic rat liver cell fractions
Experiments on rats have shown that ethanol at a dose of 4.5 g/kg leads to an increase in the activity of lactate dehydrogenase (LDH) in rat liver cells in a direct reaction in subcellular fractions. In the cytoplasmic fraction, the LDH activity in the reverse reaction decreased. Pre-administration of heparin to animals at a dose of 250 IU/kg partially neutralizes the effects of intraperitoneal administration of ethanol, which is expressed in a decrease in the activity of LDH in rat liver cells in a direct reaction in subcellular fractions. Binding of endogenous heparin to protamine sulfate and subsequent administration of ethanol leads to an increase in LDH activity in the mitochondrial fraction of rat liver cells.