CHROMOSOMAL ABERRATIONS IN THE CULTURED LINE OF HUMAN RENAL CLEAR CELL CARCINOMA
Tumor drug resistance is an actual problem for fundamental and clinical oncology. To solve it, molecular genetic study of the stable cell lines is required. The aim of the work is cytogenetic analysis and karyotype characteristics of renal clear cell carcinoma line Рпоч1-KK. Several subclones with different modal karyotype, numerous chromosome rearrangements and derivatives were detected in the cells using FISH assay. These data indicate the accumulation of various genetic alterations in tumor cells, including changing the copy number of individual chromosomes, and chromosome fragments with subsequent aneuploidy and increased genetic instability. This is one of the causes of drug resistance for renal carcinoma.