LOCAL CYTOKINE STATE BY ADENOVIRAL EYE INFECTION
69 patients with adenoviral eye infection were followed up. 27 patients represented A group (acute infection) and 42 - chronic infection (B group). The control group was represented by 39 healthy volunteers. In all cases, the diagnosis was confirmed by adenoviral DNA detection in polymerase chain reaction (PCR) (conjunctival scrape). mRNA cytokine expression was evaluated by reverse transcription and PCR. Cytokine quantity was estimated by the flow cytofluorometry method. We analyzed the quantity of 9 cytokines and the expression of 11 cytokine genes in conjunctival scrape on the 1st, 7th and 14th day. IFN-α, IFN-γ, IL-2, IL-10, IL-12, IL-18 mRNAs rates were increased in A and B groups vs control group on the 1st day, besides, mRNA IL-1β rate was decreased in both groups vs control. We noted that IFN-α, and IFN-γ gene expression rates changed in different ways with time in acute group in comparison to chronic one. In group A, IFN-α and IFN-γ mRNA expression rates were increased by the 7th day, while in the group B, we observed cytokine gene rate decline from the 1st to the 14th day. The quantitative analysis showed the decrease in all cytokine quantities in groups A and B vs control group.