Features of dynamic morphological changes in soft tissues when using sodium hypochloritefor the local treatment of purulent woundsin the maxillary region in the experiment
Background. The results of the experiment on the introduction in practice of combined complex therapy in combination with a double treatment with a freshly prepared solution of sodium hypochlorite purulent wounds of the maxillary region are presented. It has been established that the inclusion of local therapy of purulent wounds in the maxillary region with a freshly prepared sodium hypochlorite solution only moderately improves the qualitative characteristics of the morphological characteristics of the purulent wound. At the same time, even on the 10th day after the animals were introduced into the experiment, dermis and deep subcutaneous tissues show signs of alternative and exudative phases of inflammation in the form of diffuse infiltration by leukocytes and histiocytic cells, which testifies in favor of a combined effect not only on the surface layers of purulent wounds, but also to deeper sections of the paraulnary region, where, as a rule, the main tendency of the inflammatory process is solved - either reduction and reverse development, or further spread and generalization of infection. Aim. To study the features of dynamic morphological changes in soft tissues using sodium hypochlorite for local treatment of purulent wounds of the periarticular region in the experiment. Materials and methods. We conducted an experimental study to study a number of morphological reactions of soft tissues of the maxillofacial region in 20 experimental animals-rabbits of the breed "Chinchilla", weighing from 2,0 to 2,5 kg. Animals were kept in a certified vivarium on conventional feed in accordance with the provisions of the guidance document of the agro-industrial complex " Guidelines for the content of laboratory animals in vivariums of research institutes and educational institutions (RD-APK 3.007. 02-9). Phlegmons-pilled inflammation of the periarticular soft tissues in experimental rabbit animals was reproduced by the following method: a suspension containing 1 billion microbial bodies of the Museum strain of Staphylococcus aureus in 1 ml was injected subcutaneously under local infiltration anesthesia with a half-percent lidocaine solution into the pre-shaved submandibular and sub-chin areas. Animals for 30 minutes before the introduction of the experience intramuscularly injected "Zoletil-100" at the rate of 8 mg per 1 kg. weight. Results and discussion. Combined complex therapy in combination with double treatment with a freshly prepared solution of sodium hypochlorite on the surface of purulent wounds of phlegmon of the periarticular region of experimental animals showed that, compared with previous series, inflammatory and destructive changes in tissues compared with the intensity of cellular and structural changes compared with the initial period of observation are significantly less pronounced. The analysis of all morphological material confirmed our assumption that local irrigation with sodium hypochlorite, which was used by us in the complex therapy of experimental spilled purulent-inflammatory process of soft tissues of the periarticular region has a positive effect exclusively on the surface tissue structures of the skin in the form of reducing the activity of inflammatory-destructive changes. Conclusion. Morphological studies have shown that the traditional complex therapy of purulent soft tissue diseases - "surface" phlegmon has insufficient effectiveness and affects only the surface tissue structures of the skin. what is manifested by a decrease in the activity of inflammatory and destructive changes and in the deep layers of the dermis of the skin and in the subcutaneous muscles by the end of the experiment there are still diffuse inflammatory phenomena. The use of complex therapy in combination with local irrigation of purulent wounds of the parotid region with sodium hypochlorite also did not lead to a noticeable improvement in the dynamics of local inflammatory and destructive phenomena in the tissues.