INFLUENCE OF CHEMICAL TREATMENT ON GLASS SURFACE TOPOGRAPHY DURING ITS PREPARATION FOR BIOMOLECULES IMMOBILIZATION
The efficiency of biosensors as analytical devices is determined by the properties of substrate surface, such as chemical homogeneity and surface roughness. This article presents the results of topography study of the glass surface which is a material most applicable in biosensors production. Some types of glass were studied by atomic force microscopy in air and in real time mode. It was shown that the surface topography changes in the course of chemical treatment due to the involvement of surface layers in dissolution, condensation and sorption. The treatment of glass with mineral reagents significantly increases the surface roughness at the micro level and decreases at the nanoscale. Some recommendations for glass substrates preparation before biomolecules immobilization were formulated.