Differences in fatty acid profiles of erythrocyte membranes related to tumor localization in colorectal cancer (pilot study)
Aim of work is to study the compositional features of fatty acids (FA) of erythrocyte membranes in patients with different localization of the tumor in colorectal cancer (CRC). Materials and methods. Using a chromatography-mass spectrometric system (GC/MS) Agilent 7000B based on three quadrupoles (USA), the composition of erythrocyte (Er) membranes of 129 patients with CRC was studied: (average age 63.2±9.4 years, of which 68 men and 61 women; 25 with proximal, 98 with distal tumor localization) and 35 people in the comparison group. Results. A greater degree of decrease in the levels of saturated, monounsaturated FAs (MUFAs), and, conversely, an increase in the levels of polyunsaturated FAs (PUFAs) in patients with distal tumor localization compared to healthy ones, was established than the same ratio in the pair “proximal localization of tumor RCC - healthy”. A greater degree of increase in the level of omega-3 PUFAs was noted than omega-6, which affected the ratio n-6 / n-3, which was significantly reduced in cancer patients, to a greater extent with distal tumor localization. The most significant for distinguishing tumors located in different parts of the intestine were: saturated fatty acids - myristic C14:0 (p<0,001) and pentadecanoic C15:0 (p=0,012), omega-3 a-linolenic (C18:3; n-3) (p=0,02), whose levels were significantly higher and, conversely, most of the omega-6 PUFAs (C18:2 n-6, C20:3 n-6, C20:4 n-6) and one omega-3 PUFAs - C22:6 n-3 (p<0,05), whose levels were significantly lower with proximal localization of the tumor than with distal. Conclusion. The results obtained indicate the importance of taking into account the localization of the tumor in patients with CRC when conducting studies of metabolic profiles.