Generation and characterization of the VP1 recombinant protein of the chicken anemia virus
The optimized sequence of the VP1 gene fragment of chicken infectious anemia virus was cloned into the expression vectors pET15b and pGEX23T inframe fused with polyhistidine and glutathione Stransferase tags, respectively. E. coli strains Rosetta (DE3) producing recombinant 6HIS ΔVP1 and GST ΔVP1 proteins were obtained. The purification of the recombinant 6HIS ΔVP1 protein by Immobilized Metal Affinity Chromatography was worked out. The conditions of refolding, which ensure a specific interaction of the protein with the control polyclonal antibodies, were assembled.