INHIBITION BY GRAPE POLYPHENOLS COMPLEX JNK KINASE ACTIVITY IN THE ISOLATED HEPATOCYTES OF RATS
Activation of JNK kinases that are involved in signaling pathways that regulate growth, cell differentiation and apoptosis was observed in toxic lesion of liver, non-alcoholic steatosis, cirrhosis and other liver diseases. Plant polyphenols can inhibit the activity of JNK kinase in the liver cells. The aim of this work was a comparative study of the effect of quercetin and grape polyphenols complex on lipids level and activity of JNK-kinase in isolated rat hepatocytes. Incubation of isolated hepatocytes in the presence of acetaminophen (JNK activator), accompanied by the increase of transaminases activity in the hepatocytes incubation medium, the increase of triacylglycerols, free fatty acids content and phospholipids level decrease. Grape polyphenols and quercetin normalized these indicators. Acetaminophen stimulates p-JNK formation, while introducing into the incubation medium quercetin or grape polyphenol concentrate what significantly reduces the level of p-JNK and suggesting inhibition of JNK.