INVESTIGATION OF SYNTHETIC ACTIVITY OF HONDROBLASTES DEPENDING ON PASSENGING DURATION IN VITRO
The need for a sufficiently long cultivation of the primary isolated cells for the production of a certain volume of cell mass for use in the field of cellular technologies raises the question of determining the optimal period for the use of cell lines. To do this, the effect of prolonged cultivation of cell cultures on proliferation, differentiation, telomere length, phenotype and morphology of these cells is evaluated. Since, in the literature, no studies of the synthetic features of chondroblasts were found depending on long-term travel, we undertook a study of the ability of the chondroblasts of the vertebral growth plate to synthesize the glycosaminoglycans of the extracellular matrix (uronic acids and sulfated glycosaminoglycans) in the growth dynamics of the culture. Investigating the culture media obtained by the transplantation of the non-pedigree puppy’s chondroblasts (2 months), it was found that the peak of the synthetic activity of the chondroblasts is in the interval from the 1st to the 6th passage. Thus, based on literature data and our results, chondroblasts lose their morpho-functional characteristics during prolonged passage and reduce their synthetic activity. In this regard, it is more appropriate to use them for cell and gene therapy in early passages.