THE IMPACT OF THE TEMPERATURE ON CHROMATOGRAPHIC SEPARATION OF BENZALKONIUM CHLORIDE HOMOLOGS
Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» of the Ministry of Health of the Russian Federation, 127051, Moscow, Russia Abstract: The impact of the temperature on chromatographic separation of benzalkonium chloride homologs on nitrile and phenyl sorbent columns was investigated. Increasing the chromatographic temperature up to 45-60°C allows to reduce the analysis time and increase the sensitivity of benzalkonium chloride homologs detection. Increasing the analysis temperature has no significant impact on the resolution between the peaks of benzalkonium chloride homologs. However, it should be noticed that the duration of use for the columns with nitrile sorbents at higher temperatures (above 60°C) require further study, taking into account the possibility of nitrile group hydrolysis at high temperatures. Increasing the temperature of nitrile columns improves the chromatographic separation parameters for benzalkonium chloride homologs on phenyl columns.