Modified acrosome staining technique for murine spermatids
The article presents modified acrosome staining technique for murine spermatids. Touch smears were fixed by Shabadash’s method. The specimens are better stained when the ions of copper predominate in the fixing fluid unlike in classical Shabadash’s solution with calcium iones. Also, the optimal conditions for all stages of acrosome staining (fixation, oxidation, staining with Schiff-reagent, staining cell nuclei with hematoxylin and embedding) are given for the purpose of obtaining a clear result and acrosome visualization.