EXPERIMENTAL JUSTIFICATION OF THE USE OF BIOINGINEERING CONSTRUCTION FOR OCCLUSIVE VASCULAR DISEASES TREATMENT
Objective: development, production and experimental use of bioengineering construction for the treatment of occlusive vascular diseases. The developed bioengineering construction consists of a frame and fixed to it a live cell culture. This frame may be made either of metal or polymeric material. In a most preferred embodiment, the construction frame may be formed as stent in which the stiffening ribs are provided with fixed cell culture wells. The above-mentioned live cell culture preferably comprises autogenic or allogenic cells, which can be fibroblasts or multipotent mesenchymal stromal cells. A stent implantation in experimental animals (7 mongrel dogs) was performed under intravenous anesthesia. A selective angiography was performed in all experiments 70 days after operations to assess the patency of stents coated with cultured fibroblasts. It was a good positioning of the stent and there were no visible deformation of the arteries or distal thrombosis. While, none of the dogs received disaggregative or other therapy. All experimental animals underwent angiography and were euthanized. The aortal parts with the renal arteries and kidneys were got after autopsy. The stented parts of renal arteries were endothelializied even layer. On a section, we could clearly see location of the stent cells relative to the vessel wall thickness.