PROCEDURE OF THE ABSOLUTE ACCOUNT OF DIGGING FORMS AMPHIBIANS
The methods of the absolute consideration of digging forms of amphibious ( Pelobatidae, Bufonidae ) which give the opportunity to evaluate various functional roles in ecosystems are depicted in this work. The different methods are taken into consideration such as a method of pipettings, a method of the areas with the double trenches, a method of limited areas. Due to these method the data of the quantity account, of amphibious are true with the probability of 80 to 98 % are considered. The offered method differs from others by the high degree of biological ethics and by getting the information on population structure a place of migration and natural death rate of population. Functions of amphibians in ecosystems can be assessed only knowing their absolute number. There are a number of methods, but they focus on relative size, methods of counting animals that lead a terrestrial lifestyle. These methods do not give information about the animals that are nocturnal, subterranean way of life, the majority of the time are in the soil or litter and carry out the digging activity. Therefore, the development of counting the number of such species is particularly important. Pelobates fuscus and Bufo bufo are the types who lead a hidden life and carry out digging activity. Various techniques and methods of determination have been material to the conduct of these studies explore the absolute abundance of digging forms amphibians. The research was conducted at the International Biosphere station as part of an complex expedition Oles Honchar Dnipropetrovsk National University. Various techniques absolute accounting was carried out in optional sections: using traps trenching, digging method, fenced plots and active way in various ecosistem. In studies take into account the peculiarities of different groups microstreaming. The proposed methods absolute accounting digging forms of amphibians meet the main requirements for many species and environmental research in the field of batrachotoxin. These methods correspond to the modern methods of biological research and based on the principles of biotic ethics using in vivo methods of research with a full set of necessary information.