Expression of Long Non-Coding RNA PRINS and G1P3 in Children with Psoriasis
Purpose. To identify and compare the expression of PRINS and G1P3 in children with psoriasis depending on the clinical-epidemiological features of the pathological process and to study the relationship between PRINS expression and G1P3 expression. Materials and methods. 56 children with psoriasis aged from 4 to 17 years were examined. Psoriasis was diagnosed on the base of clinical data and generally accepted diagnostic criteria. PRINS and G1P3 expression levels were determined in psoriatic keratinocytes and buccal epithelium through reverse transcription and real-time PCR. Psoriasis severity indices (BSA, PASI, PGA) and body mass index (BMI) were calculated. The study materials were statistically processed using the STATISTICA 13.3 software. Results. In children with psoriasis, statistical differences were found between the expression of PRINS in the buccal epithelium and in keratinocytes affected by psoriasis (p=0.034). Similar differences in PRINS expression were found in the group of girls (p=0.038), in the group of children with BSA >10 (p=0.019) and in the group with PGA=4 (p=0.017), in the group of children with the signs of onychodystrophy (p=0.009) and exacerbation of psoriasis for more than 5 weeks (p=0.017). The relationship between the expression of PRINS and peculiarities of the clinical manifestations of psoriasis was revealed, which is reflected in the increase of the area of skin lesions by 1% and increase of the intensity of skin manifestations by 0.29 with the increase of PRINS expression in the buccal epithelium by 0.207. G1P3 expression in psoriatic keratinocytes is 0.88±0.26. Statistical differences in G1P3 expression were revealed when comparing the groups by gender, skin lesion area (BSA), intensity of clinical signs (PGA), and BMI. Conclusion. Dysregulation of PRINS in the pathogenesis of psoriasis was confirmed. It was found that not only the level of PRINS expression in the intact epidermis is important for the course of psoriasis, but also the level of differences with PRINS expression in psoriatic keratinocytes: high PRINS expression in the buccal epithelium and low expression in psoriatic keratinocytes lead to severe psoriasis with prolonged exacerbation and unfavorable prognosis. The role of increased G1P3 expression in psoriatic keratinocytes in the pathogenesis of psoriasis was confirmed; the more severe psoriasis is in terms of the area of skin lesions and the intensity of clinical manifestations (erythema, desquamation, infiltration of foci), the higher are the levels of G1P3 expression in psoriatic keratinocytes. The relationship between PRINS and G1P3 expression in psoriatic keratinocytes is ambiguous.