Use of molecular-genetic method for evaluating intensity of morphofunctional changes in patients with dentoalveolar pathology
The method of bacteriologic investigation employing the anaerobic cultivation technique, as well as the molecular-genetic method based on polymerase chain reaction, were used to detect the major parodontopathogenic species and to conduct quantitative evaluation of parodontopathogenic and residential microfl ora in gingival sulcus biofi lm in children and adolescents (aged 7-14) with dentoalveolar anomalies. It has been shown that a proper indicator best refl ecting the intensity of morphological and functional disturbances in case of dentoalveolar anomalies in children and adolescents, is an increase of parodontopathogene genetic markers in gingival fl uid (Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Bacteroides forsythus).