STIMULATION OF TRANSLATION OF REPORTER mRNA IN THE PRESENCE OF ANOTHER mRNA IN A CELL-FREE SYSTEM BASED ON WHEAT GERM EXTRACT
Translation of non-capped mRNAs encoding luciferase and green fluorescent protein in a cell-free translation system based on wheat germ extract has been studied. It turned out that two simultaneously translated (in one tube) different templates in a certain range of concentrations not only do not compete but mutually enhance translation of each other. It has been shown that the synthesis of luciferase in the presence of mRNA encoding green fluorescent protein is much more effective than in the case of translation of only luciferase mRNA at the same concentration. And in the same way, the efficiency of synthesis of green fluorescent protein increases in the presence of the template encoding luciferase. It follows that the total effect of concurrent translation of two different mRNAs exceeds the sum of the effects of translation of each mRNA separately.