MANNOSE-BINDING LECTIN GENE POLYMORPHISM IN RUSSIAN FAR NORTH ABORIGINAL NEWBORNS AS FACTOR PREDISPOSING TO SEVERE COURSE OF BACTERIAL INFECTIONS
We have done the estimation of the genotypes frequency predisposing to severe course of bacterial infections in different ethnic populations of indigenous newborns in Russian Arctic region on the basis of analysis of mannose-binding lectin gene (MBL2) polymorphism. We studied the prevalence of the two common deleterious variants (rs1800451 and rs1800450) in aboriginal and alien populations of the Taymyr-Dolgan-Nenets Region of Krasnoyarskiy Kray, East Siberia, Russia (Nenets, Dolgans, Nganasans, Russians) using 586 dry blood spots. DNA was extracted using DIAtomTM DNAPrep kits. Genotyping was carried out using restriction fragment lengths polymorphism (RFLP) approach. It was found that Dolgan-Nenets and Nenets newborns have significantly less common genotypes associated with defective structure mannose-binding lectin (AB and BB genotypes in rs1800450 MBL2 gene) compared with newborns-Caucasians (Krasnoyarsk).