Organogenesis of half-highbush blueberry during clonal micropropagation depending on lighting conditions
The article presents the results of studies on the effect of illumination of different spectral ranges on the growth and development of half-highbush blueberry plants of Northblue cultivar and the promising hybrid form 23-1-11 during clonal micropropagation. Half-highbush blueberry regenerated plants are cultivated on a WPM nutrient medium using cytokinin 2ip and auxin IAA and illuminated with white fluorescent lamps, white LED lamps and LED lamps with a combination of white, red and blue spectra. The largest number of microshoots (13.1 pcs.) of blueberry with the greatest total length (98.7 cm) is formed when the regenerated plants are illuminated with fluorescent lamps at the stage “proper micropropagation”. The root formation of half-highbush blueberry plants is 7 times more intense when the ground part of the plants is illuminated with LED lamps with a combination of white, red and blue spectra (number of roots - 9.9 pcs., total length - 114.8 cm) than in fluorescent and white LED lighting options at the stage of “rooting in vitro”. No significant differences in the number and length of microshoots and roots of half-highbush blueberry, depending on the cultivar are observed during clonal micropropagation.