OPTIMIZATION OF CONDITIONS FOR OBTAINING LIPOSOMAL FORMS OF A CONCENTRATE OF POLYUNSATURATED FATTY ACIDS
The research is concerned with optimization of conditions for obtaining liposomes with a concentrate of polyunsaturated fatty acids (PUFA). We obtained the concentrate from seal fat by method of complexing with urea. The analysis of the concentrate showed high essential PUFA content with a ratio of ω-6:ω-3 of 1,3:1. Via an experiment we detected an optimal proportion of PUFA concentrate and phospholipids for obtaining liposomes. We also identified the optimal period of liposomal structures storage. We studied the processes of lipid peroxidation in liposomes. We found out that during the process of storage the level of TBA-active substances in the products of lipid peroxidation did not rise. We identified the degree of PUFA concentrate inclusion in liposomes at 62.6 %. We defined the size of liposomes by method of turbidimetry. The average diameter of obtained liposomes is 99.54 nm.