THE PATHWAYS AND MECHANISMS OF IMMUNE CELL DEATH IN THE TIME COURSE OF EXPERIMENTAL IMMUNE-MEDIATED LIVER INJURY
In this work the apoptotic and necrotic death of cells isolated from primary and secondary lymphoid organs during the course of ConA hepatitis in mice has been investigated. Experiments showed the differences in the pathways of cell death of thymic and splenic lymphocytes. Elevation of apoptosis has been observed earlier than the increase in necrosis in the cells isolated from thymus. A significant increase in necrosis of splenocytes occurred early after ConA treatment, however elevation in the number of apoptotic spleen cells has not been established. Treatment of mice with inhibitor of NF-kB andrographolide resulted in decrease in both necrosis and apoptosis, induced by ConA. These results suggest a role of NF-KB in induction of immune cells death during Con A-induced immune hepatitis.