Strains of Bacillus Regulate Wheat Resistance to Greenbug Aphid Schizaphis graminum Rond.
The ability of bacteria Bacillus subtilis Cohn. (strain 26 D) and Bacillus thuringiensis Berliner (strains V-6066 and V-5689) to negative influence on the livelihood of greenbug aphid Schizaphis graminum Rond. has been detected. Protective effects of the studied strains of Bacillus spp. consisted in direct aphiciyde activity and indirect induce systemic resistance in wheat plants by the regulation of hydrogen peroxide generation, activity of peroxidase and catalase and an increase expression of defense related genes such us NADPH oxidases, PR-6, and PR-9. It has been shown that B. thuringiensis strains induced genes of jasmonate-dependent signaling pathway, and B. subtilis strain induced genes of salicylate-dependent signaling pathway. It has been found that the composition of the studied strains of Bacillus spp. had an additive effect of wheat plant resistance to pest. These results suggest use these strains Bacillus spp. as a basis for further development of bio preparation against cereal aphids.