MEASURING MICROFLUIDIC SYSTEM FOR THE MAMMALIAN BRAIN CELLS CULTIVATION
Prototyping and development of microfluidic systems for many non-specialized biomedical research laboratories are not available due to the high cost of the necessary equipment. One of the interesting directions of using microfluidic platforms is the modelling of the blood-brain barrier and the neurogenic niche of the brain in vitro. The solution of technological problems within this area will ensure progress in the development of new diagnostic and therapeutic technologies for diseases of the central nervous system. Given that the cells of the neurovascular unit of the brain (cerebral endotheliocytes, perivascular astrocytes, neurons, pericytes) are very sensitive to culturing conditions, the requirements for the micro-stream system for their survival, development and functioning are quite high. Purpose of work: to conduct a full cycle of manufacturing a microfluidic flow chamber for culturing cells available for biomedical measurements, and to coordinate the microfluidic platform with a sufficient set of peripheral equipment. We have developed a method for transferring a positive pattern formed by milling from the surface of polymethylmethacrylate to the surface of polydimethylsiloxane. It was found that dental silicone mass for duplication Zhermack Elite double 22 models has poor adhesion at the interface between the long-frozen layer and the freezing layer. This made it possible to double take a replica from the canalized surface and obtain a copy of the milled canalized surface on the elastic material. We have shown that the microfluidic chip obtained by our method can be useful for cell cultivation.