The state of energy metabolism in patients with diabetes mellitus type 1, complicated by ketocyadosis
Aim . Study of the content of non-esterified fatty acids, glycerol in plasma, and adenylic nucleotides in erythrocytes in patients with type 1 diabetes mellitus complicated by diabetic ketoacidosis. Materials and methods. The clinical material was quantitative indicators of the content of adenyl nucleotides in red blood cells in 198 patients with type 1 diabetes mellitus. All patients were divided into three groups. The first group consisted of 68 patients with type 1 diabetes mellitus, without diabetic ketoacidosis; the second, 88 patients with type 1 diabetes mellitus, complicated by mild diabetic ketoacidosis; the third - 42 patients with type 1 diabetes mellitus, complicated with moderate and severe diabetic ketoacidosis. Аdenosine triphosphate concentrations in erythrocytes were determined by the method of P.M.Yaverbaum et al. (1984). The concentrations of adenosine diphosphate and adenosine monophosphate in erythrocytes were determined by the method of H.U.Bergmeyer (1965). To determine the overall level of non-esterified fatty acids used a colorimetric method for the determination of copper salts. Results. In patients with type 1 diabetes mellitus complicated by diabetic ketoacidosis, significant changes in the adenyl system were found, characterized by a significant decrease in the content of adenosine triphosphate and adenosine diphosphate in erythrocytes, while simultaneously increasing the concentration of adenosine monophosphate. Conclusions. Diabetic ketoacidosis leads to an energy deficit in the myocardium. The indicator of the degree of damage to the energy metabolism of cells can be considered as the level of adenyl nucleotides and, above all, adenosine triphosphate, reflecting the final stage of energy accumulation by tissue