GROWTH OF CLOVER AND ALISSUM <i>IN VITRO</i> IN MEDIA WITH HIGH HEAVY METAL CONTENT
Study deals with the growth and antioxidant reaction of creeping clover plants, growth two species of Alyssum grown in an aseptic culture in a media with a high content of copper and nickel ions. The study is aimed at selecting plants promising for use in phytoremediation. Clover plants were obtained by cell selection, alyssum plants - from seeds harvested in natural habitats and propagated microclonally in an aseptic culture. The choice of plants was due to the fact that clover is a cosmopolitan species, and Alyssum species are known as nickel hyperaccumulators. It was shown that T. repens lines obtained by cell selection have a lower growth rate compared to the original ones. The addition of 200 μM copper ions to the cultivation medium did not significantly affect the growth of selected forms, whereas control plants were inhibited. Alyssum tortuosum was shown to be more resistant to excess copper and nickel ions than Ayissum litvinovii . The addition of 200 μM Ni<sup>2+</sup> to the medium did not show effect on the growth of alyssum plants, and 100 μM Ni<sup>2 +</sup> even stimulated growth. Genotypes that survived on 200 μM Cu<sup>2 +</sup> in the medium were found, which may be promising for their use in soils with polymetallic contamination.