Immuno-Histochemical and Morphometric Characteristics NPY-Positive Neurons of the Different Fields of Human Cerebral Cortex at the Chronical Ischemia
Immuno-histochemical (NPY - neuropeptide Y) and morphometric study of the distribution and architectonics of NPY immune-positive brakes of interneurons layer III of various fractions (field 4, 10, 17 and 21) of neocortex human normal (n=5) and chronic ischemia (n=15, perhifocal area tumors) was carried out. Verification of NPY-positive brake interneurons held on the content of NPY, the shape of their bodies and processes. In all studied fields in norm and under ischemia the basket cells, Martinotti cells and releasing secret of inter-neurons were identified. It was established that in case of chronic ischemia in all the studied fields cortex of the relative size of NPY-immune-positive structures (labelled bodies of neurons and their appendages) are statistically significantly higher than in control: in field 10 - in 1.95, in field 4 - 1,85, in field 21 - 2,17, in field 17 - 2,15 times. The received results testify to a compensatory increase in the expression of NPY in the intact interneurons of perifocal area of human neocortex with chronic ischemia. Increase in the volume of NPY- immune-positive material was due to the expansion and thickening of the processes of interneurons. The authors examine the hypothesis about the possible role of activation and hypertrophy of NPY- immune-positive interneurons in the regulation of the balance of brake systems and exciting damaged brain cortex and as one of the mechanisms for the protection of neural networks from chronic ischemic damage.