PERSPECTIVES OF INVESTIGATION OF MAJOR SALIVARY GLANDS SECRETION BY CLASSIC CRYSTALLOGRAPHY METHOD IN URBANIZED REGIONS POPULATION
The purpose of the investigation was study of major salivary glands secretion by classic crystallography method in patients with type 2 diabetes living in relatively clean Centralnyi area and industrial Levobereznyi area. Materials and methods. Anthropotechnogenic load has been analyzed by data of «Centre of hygiene and epidemiology of Voronezh region». Indices of atmospheric air, water and soil pollution have been examined by Bushtueva’s formula. 81 people were observed: 40 practically healthy people (control group) and 22 patients with type 2 diabetes from and industrial area and 19 from relatively clean area. Secretion of left and right parotid (LP, RP), submandibular and sublingual (SMSLSG) salivary glands were obtained with the help of saliva trap in the morning. For analysis of biological samples classic crystallography method had been used. 5 mkm of each secretion were localized onto quartz glasses and dried at the temperature 250C. Video-Test program has been used for morphometric analysis of cristallograms. Results. According to environment pollution ecologically troubled Levobereznyi region and normal sanitary-hygienic Centralnyi region were distinguished in Voronezh. The main role in complex anthropotethnogenic load belongs to atmosphere pollution. By morphometric analysis of crystallogramms left and right parotid (LP, RP), submandibular and sublingual (SMSLSG) salivary glands secretion valid increase of intermediate layer width from 105.25±11.11 up to 160.13±18.94 mkm and optical density of transitional layer from 0.44±0.07 up to 0.64±0.13 standard unit in patients with type 2 diabetes have been observed (p < 0.05). What is more, patients from industrial area had more statistically valid differences than patients from relatively clean area. Conclusions. Differences in layer’s width and optical density indicates the interaction of biochemical blood composition and components of major salivary glands that effects on crystallization processes. It may be diagnostic feature of diabetes and anthropotechnogenic load, too.