OBTAINING AND CULTIVATION OF STEM CELLS FROM THE UMBILICAL CORD AND OTHER HUMAN TISSUES. BRIEF SUMMARY AND INITIAL EXPERIMENT
The results of experiments obtained by different authors in solving problems of functional restoration of damaged tissues and organs using cell therapy and tissue engineering are discussed. The results of investigations devoted to the study of the efficiency of obtaining a stem cell culture with a high level of proliferation are presented. The problem of using tissue engineering and cell therapy in restorative treatment of damaged tissues and organs is briefly discussed. Various researchers have shown that the umbilical cord tissue has the greatest regenerative potential of allogeneic substitution tissues. Preliminary results of our own research on the assessment of the potential and intensity of stem cell proliferation after explantation of tissue fragments of Wharton's jelly are presented. The results of experiments obtained by different authors in solving problems to functional repair of damaged tissues and organs using cell therapy and tissue engineering are discussed. The results of research on the effectiveness of obtaining a stem cell culture with a high level of proliferation are presented. The problem of using tissue engineering and cell therapy in the regenerative treatment of damaged tissues and organs is briefly covered. The analysis of the literature shows the possibility of initiation of human cell culture and the efficiency of its further cultivation. The results of introduction into culture and possibilities of further cultivation of cells from bone marrow tissues and increase of cultivation efficiency at changing of environment composition are given. The possibilities of application of adipose tissue as an alternative tissue of bone marrow source of stem cells are shown. Methods of obtaining and chondrocytes from cartilage tissue using mechanical enzymatic method, as well as conditions for their further cultivation are presented. The methods of introducing limbal epithelium into cell culture, providing reparative regeneration of the eye cornea, are briefly described. Examples of obtaining cell culture from the pulp of a tooth extracted from the pulp chamber cavity after surgical removal of a tooth are given. Different researchers have shown that umbilical cord tissue has the greatest regenerative potential for replacement allogenic tissues. The methods of obtaining cell culture from both fragmented whole umbilical cord, umbilical cord blood and perivascular space of umbilical vein are presented. Methods of obtaining and cultivating cells from warton jellybean tissue are discussed. Preliminary results of our own research to estimate the potential and intensity of stem cells proliferation after transplantation of fragments of Wharton's jelly tissue are presented.