INFLUENCE OF SODIUM SELENITE ON THE VIABILITY OF MICE TESTICLE CANCER CELLS AND THE EXPRESSION OF MRNA OF SELV (SELENOPROTEIN V), TGR (THIOREDOXIN-GLUTATHION REDUCTASE) И GPX4 (GLUTATHION PEROXIDASE 4) IN THEM
One of the most common selenium compounds, considered as a potential anticancer agent capable of inducing the formation of ROS, inhibiting cell growth, influencing the expression of pro-apoptotic genes, etc., is sodium selenite. In this paper, the effect of various concentrations of sodium selenite on the viability of mice testicle cancer cells and its role in the regulation of mRNA expression of three mouse selenoproteins localized in the testicle and involved in spermatogenesis of mammals has been investigated. Given the important role of selenoproteins in the regulation of carcinogenesis, primarily as antioxidants, the results of this work are very relevant. A significant decrease in the viability of F-9 cancer cells was observed with 7.5 and 10.0 μM SS. The real-time PCR method revealed that the processing of SS cells resulted in an increase in the expression of GPX4 mRNA and a significant decrease in the expression of TGR mRNA. These results are inconsistent with the previously obtained data on the joint functioning of the two enzymes in the testes and suggest that TGR may have oncogenic properties. SELV mRNA was not detected either before or after the SS action on cells, so it is too early to talk about its participation / non-participation in malignant transformation processes. The results obtained by real-time PCR were also confirmed by Western blot analysis and can be considered as an initial stage in the study of the role of SELV, TGR and GPX4 selenoproteins in carcinogenesis.