The effects of cycloferon on the distribution of neuroamines in bioamine-containing structures of the spleen
The article describes the distribution of neuroamines in bioamine-containing granular luminescent cells and mast cells of different zones of the spleen when a widely used immunomodulator, cycloferon, is administered in a different temporal aspect. Cycloferon is the inducer of the synthesis of endogenous interferons. Laboratory animals were injected with cycloferon into the tail vein with a multiplicity of five and ten injections. Cryostat sections of the spleen were treated with luminescent histochemical methods to detect biogenic amines (histamine, serotonin, catecholamines). After a five-fold administration of cycloferon, the content of histamine and serotonin in the granular luminescent cells of different areas of the white pulp tends to decrease, while the accumulation of these bioamines of the granular fluorescent cells of the red spleen pulp. This number of injections contributes to the activation of mainly in-zones of the white pulp of the spleen, responsible for the humoral link of immunity. After a 10-fold administration of cycloferon, the content of histamine and serotonin in the GLA of the white and red pulp changes unidirectionally, while there are changes in the T - and In-zones of the spleen, which leads to the simultaneous activation of cellular and humoral immunity. With a fivefold administration of cycloferon, activation of mainly the B-zones of the white pulp of the spleen responsible for the humoral immunity unit is observed. With a tenfold administration of the drug, changes are observed in the T and B zones of the spleen, which leads to simultaneous activation of cellular and humoral immunity. The index of the serotonin index at 5-fold and 10-fold administration of cycloferon is less than one, which determines the higher content of catecholamines in the cellular structures in comparison with serotonin and can be evidence of a strong control of the immunogenesis processes in the spleen from the side of the adrenergic link of the ANS.