Diagnostic significance of clinico-laboratory cyclase system indices in leukocytes of the patients suffering from diabetes mellitus type 1 and 2
Fifty two patients with evident diabetes mellitus type 1 and 2 and 19 healthy male control subjects were examined in order to assess a diagnostic value of clinico-laboratory cyclase system indices in leukocytes during insulin tolerance test. The patients suffering from diabetes mellitus 1 type and healthy subjects showed increased ratio cyclic adenosine monophosphate/cyclic guanosine monophosphate; the initially confident high ratio of cyclic nucleotides in the patients suffering from diabetes mellitus 2 type is considerably lowed mainly due to different changes in the concentration of cyclic adenosine monophosphate. The high degree of immunoreactive insulin concentration increase is revealed during insulin tolerance test in the patients suffering from diabetes mellitus 2 type as compared to the patients with diabetes mellitus 1 type and the healthy subjects. This increase is accompanied by unusual reaction of leukocyte cyclic nucleotides due to pronounced lowering of cyclic adenosine monophosphate concentration. High degree of insulin level increase and pronounced decrease of the ratio cyclic adenosine monophosphate/cyclic guanosine monophospahte during insulin tolerance test are suggested to be considered as clinico-laboratory signs of tissue insulin resistance in the patients suffering from diabetes mellitus 2 type.