EFFECT OF BLACK CUMIN OIL ON VARIOUS COMPONENTS OF HAEMOSTASIS IN RATS WITH EXOGENOUS THROMBINEMIA
Black cumin oil has a rich phytochemical composition and is widely used as a dietary supplement for preventive purposes. However, there is a lack of scientific data on its effect on haemostasis. Thus, the purpose of this paper was to study the nature of the effect of the dietary supplement Ethiopian Black Cumin Oil on the platelet and coagulation components of the coagulation system under experimental thrombinemia. Materials and methods. The research involved 60 white non-inbred male rats. The following haemostasis parameters under exogenous thrombinemia were studied: prothrombin time, thrombin time, activated partial thromboplastin time, content of fibrinogen and soluble fibrin monomer complexes, antithrombin III activity, total platelet count and the ratio of different forms of platelets. Results. The research showed that pre-administration, in the course of 21 days, of black cumin oil to rats (experimental group) increases their tolerance to thrombin, limiting excessive platelet activation, development of thrombocytopenia and consumption coagulopathy compared with the control group. The data obtained during the experiment revealed a protective effect of oral administration of black cumin oil under provoked hypercoagulation stress. Сonsumption coagulopathy in the test group was less pronounced after 0.5 hours of experimental thrombinemia; after 1 hour, haemostasis parameters returned to baseline values at a faster rate compared to the control. The supposed mechanism behind this effect is the ability of the antioxidants in this dietary supplement to limit the processes of free radical oxidation: black cumin is a combined natural antioxidant rich in flavonoids, phenolic acids, tannins and terpene compounds. The results of the research indicate good prospects for further studies into the effect of the dietary supplement Ethiopian Black Cumin Oil on various components of haemostasis as a non-specific agent.