Specific binding of substances of insecticides of the neonicotinoid group with human albumin and alpha-1-microglobulin
In biochemical systems in vitro, the specific binding of the known commercial insecticides of the neonicotinoid group such as imidacloprid, clothianidin, acetamiprid, thiamethoxam, and thiacloprid with human plasma transport proteins has been established It has been shown that imidacloprid by the competitive mechanism displaces native ligand estradiol from the binding site of human serum albumin and from ‘‘lipocalin pocket” of human alpha-1-microglobulin (A1M). It has been found for the first time that A1M, a member of the lipocalin family, can specifically bind xenobiotics. By fluorescence spectroscopy it has been established that neonicotinoids form equimolar complexes and the association constant values fall into the range 10<sup>4</sup> to 10<sup>5</sup> М<sup>-1</sup>.