Microbiocenosis of oral cavity in experimental animals exposed to damaging factors of chemoradiotherapy
Experimental studies have shown that the combined effect on animals of cytostatic (cisplatin) and cranio-caudal gamma-irradiation causes a significant increase in microbial contamination of the oral mucosa. Irradiation in a doze of 10 Gy caused significant growth of number of colonies of β-hemolytic streptococcus, non-hemolytic streptococcus, staphylococci, enterobacteria, anaerobi and candidi. At the height of chemoradiation stomatitis (on the 15 <sup>th</sup> day after irradiation) the number of colonies of non-hemolytic streptococci, staphylococci, enterobacteria increased by an average of 3 times in comparison with the group of intact animals. The highest growth (5 times) was detected in yeasts C. albicans and C. glabrata. Irradiation changed a level of cationic antimicrobic peptides in peripheral blood of rats: the contents of α-defensin HNP 1-3 and cathelicidin LL-37 was reduced by 30-40%, and β-defensin hBD-3 insignificantly raised. After combined chemoradiation therapy the level of α-defensin HNP 1-3 and cathelicidin LL-37 in the blood of rats were decreased in 1,7-1,8 times, but level of β-defensin hBD-3 was increased by 70%.