Optimal Parameters for the TLC-separation of <i>Pinus silvestris </i>Seed Lipids
The thin-layer chromatography method is convenient and economical in the performance, is allowed to conduct a rapid analysis of the composition of complex lipid mixtures and apply it to assess of the quality of the seeds, favorable conditions for their growth, etc. At this stage of the study, a solvent system was selected for a more complete separation of neutral lipids and phospholipids of the pine seeds Pinus silvestris Ledeb, which is one of the dominant forest-forming species in Irkutsk region and the most important raw material. TLC carried out a preliminary assessment of the component composition of lipid fractions. The solvent system hexane-diethyl ether-acetic acid (70:30:1) was carried out by the optimal system for separation of neutral lipids of pine seeds. The best results were obtaned separating of phospholipids and using the chloroform-methanol-ammonia system (65:35:3). Ten classes of NL and nine fractions of PL were revealed.