Gel-filtration of casein phosphopeptides
Сaseins as the natural food proteins have balanced their amino acid composition and are accessible to digestive enzymes in gastrointestinal tract. Although caseins meet classical requirements to food proteins they are also known as precursors of multiple bioactive peptides. These peptides cross into the bloodstream and affect the nervous system (agonists and antagonists of opiate receptors), digestive system (casophosphopeptides, glycomacropeptide from κ-casein), immune system (immunomodulatory and antimicrobial peptides) and cardiovascular system (antithrombotic and antihypertensive peptides). Casophosphopeptides are very important bioactive peptides. They have a positive influence on the absorption of calcium, iron, zinc and magnesium ions in organism and are the perspective ingredients for the functional food. In current study the distribution of casein phosphopeptides molecular mass by gel-filtration methods has been comparative investigated. Phosphopeptides were obtaining by proteolysis of total casein, using enzyme preparations of animal, plant and microbiological origins. It has been detected that there is an important difference between phosphopeptides compositions according to the using one of various enzyme preparations.