Loop - mediated isothermal amplification of nucleic acids: principle and application
The article is devoted to the new methods of molecular diagnostics - loop mediated isothermal amplification. The components, stages of this reaction, the amplification products visualization techniques and its advantages and disadvantages are also described. The advantages of loop mediated isothermal amplification include ease, speed, sensitivity and specificity, cost effectiveness. Unlike polymerase chain reaction method, LAMP uses four (six) primer that provides a high level of specificity. LAMP carry out under conditions of constant temperature, that is, for its implementation does not require expensive equipment such as a thermal cycler. Devices on the basis of thermoblock or a water bath can use for statement of LAMP. In addition, information about the possible uses of the method for the diagnosis of bacterial and viral infections is given in the article. At present a small number of test systems for the detection of pathogens DNA and RNA were developed. The main producer of test systems is the Eiken Chemical company.