Comparison of different types of microsurgical suture for sustaniability of anastamosis in renal transplantation in rats
The purpose of our work is to compare different types of microsurgical vascular sutures, to select the most appropriate suture for kidney transplantation. For this, different types of microsurgical sutures were compared, to create arterial and venous anastomoses of the “end to side” type when performing heterotopic kidney transplantation in mammals. Our studies were carried out according to the European Convention for the Protection of Vertebrate Animals used for experiments or for other scientific purposes. The experiment was carried out on 18 white rats, which were divided into 3 groups of 6 animals each. In the first group, kidney autotransplantation was performed with anastomosis created between the renal artery and the aorta, the renal vein and the caudal vena cava of the "end to side" type using single interrupted sutures. In the second group, a continuous suture was applied, and in the third, a continuous suture using a graft with Carrel sites at the ends of the vessels. In the first experimental group, all the anastamoses obtained were distinguished by a good cross, the rate of suturing was 20 ± 1.29 min. when connecting the arteries and 23,16 ± 1,95 min. when connecting the veins, the technique of performing was the most difficult. In the second group, the vascular permeability in 2 cases was impaired, which is explained by less visual and instrumental control of the sewn vessel walls during the execution of this technique, the rate of suturing is 12 ± 0.57 min. when working with arteries and 15 ± 0.82 min. when working with veins, the technique is simpler than in the first group. In the third, the permeability of all anastamoses was good, the speed of work was the highest: 11.83 ± 1.07 min. with suture over arteries and 14.67 ± 1.1 min. veins, the complexity of the execution of the most simple, but getting a transplant with Carrel sites is possible only with posthumous donation. The use of single interrupted sutures, despite the complexity of the technique and the slower rate of application, is the most reliable method that is suitable for creating the majority of vascular anastomoses during kidney transplantation in mammals.