ANALYSIS OF MUTAGENE ACTIVITY OF THE EXPERIMENTAL MODEL OF MIROENCAPSULATED PROBIOTIC PREPARATION "ENZYMESPORINE" ON LABORATORY ANIMALS
The aim of nvestigation has been survey the mutagene activity of the experimental model of the microencapsulated probiotic preparation, which was made in scientific production laboratory of small innovative company of LLC “INNOTEKH”. The object of the investigation is microencapsulated probiotic preparation “Enzymesporine” (“The experimental model of the microencapsulated probiotic preparation”), which was made in scientific production laboratory LLC “INNOTEKH”, which “consists” Bacillus subtilis VKPM V-314, Bacillus licheniformis VKPM V-8054, Bacillus subtilis (Bacillus natto) VKPM V-12079, which have “powder-like” form, ”which consists” of parts which inclosed in polymer shell. By way of research method there was used a test for the induction of chromosomal damage in vivo in mammals and counted the number of chromosomal aberrations in cells of mammalian bone marrow in experiments on male rats (for a single intraperitoneal injection) and on bisexual rats (for prolonged injection for 5 days). As negative control was used 1% starch paste. As positive control was used Cyclophosphan. The research was conducted on laboratory rats of Wistar line, on males and females. The average weight is 250.44±20.99 g. Amount: 54 males, 12 females. The animals were kept under a 12-hour light regime with free access to food and water. Experimental rats were divided into three big groups. The number of cells with chromosomal aberrations in the group of animals treated with the test preparation in a daily subtoxic dose (tenfold therapeutic) was 1.43±0.98, which was not statistically different from the data in the negative control group (1.86±1.07 cells), and significantly different from those in the positive control group (14.29±1.98). It was concluded that the experimental model of the microencapsulated probiotic preparation “Enzymesporine” does not have mutagenic activity in the test for the induction of chromosomal mutations.