INFLUENCE OF FIELD AND VACCINE STRAINS OF AVIAN INFECTIOUS BRONCHITIS VIRUS ON CILIA APPARATUS AND HISTOMORPHOLOGY OF CHICKENS TRACHEA, LUNG, AND CHICKENS` KIDNEY
The results ciliostatic test, serological tests and histological analysis of the trachea, lungs and kidneys we have found differences in field isolates of LI-1 and LI-2 of the virus of infectious bronchitis of chickens from vaccine strains MA-5, N-120 and 4/91. Isolates LI-1 and IS-2 were isolated from poultry, respectively, in 240- and 150 days of age in two poultry farms in the Luhansk region. The authenticity of the virus isolates IBK was established by PCR and electron microscopy for typical coronavirus morphology of virions. We also used the vaccine strains of the virus IBK - 4/91, MA-5 and N-120. The study was performed on two groups of Chicks 3 days of age with maternal antibody to the virus IBK higher (3-3,75 log2) and lower protective (less than 3 log2). Each group consisted of 6 subgroups; Chicks first 5 sub-groups were infected with virus strains - subgroup of LI-1, LI-2, N-120, MA-5 and 4/91; 6th subgroup remained intact and served as controls. In Chicks without maternal antibody to the virus IBK cryostat of the trachea is observed respectively after 24 and 48 h after infection with the isolates of LI-1, LI-2 and is accompanied by the appearance of antibodies to the virus, whereas passive immunity extends manifestations of ciliostasis of the trachea and accumulation of antibody to the virus. We established an inverse correlation between ciliary activity of the trachea and the level of antibody to the virus IBK, which is more reliable after inoculation of the isolate LI-2 strain N-120. Isolates of LI-1, LI-2, and vaccine strains (MA-5, H-120, 4/91), causes inflammatory and destructive processes in the organism of chickens: a mild swelling of the actual mucous membrane of the trachea, increase in the number of goblet cells, ceciliale, destruction, detachment of epithelial cells of the trachea; acute venous hyperemia, and lymphoid infiltration of the lungs and kidneys.