Research of anti-inflammatory and anti-diabetic action of grape polyphenols on the experimental model of metabolic syndrome
Currently metabolic syndrome (MS) is one of the most common forms of worldwide pathology. Especially interesting is the study of the role of immune and inflammatory mechanisms of the pathological cascade activation in MS, in partucular through the Toll-like receptor type 4 (TLR 4), which causes inflammation and insulin resistance. Certain role in the MS pathogenesis is played by oxidative stress. Most of in vivo and in vitro studies show that consuming foods rich in polyphenols can reduce the risk of cardiovascular disease, hypertension and diabetes. The aim of this research was to study the role of inflammatory and immune mechanisms activation in the development of experimental MS and to substantiate the pathogenetic approaches for its correction using a polyphenol concentrate. Materials and methods. An experimental study was performed on 40 white male Wistar rats. The fructose MS model of solid feeding was used with a 60% fructose content for 24 weeks. To confirm the MS we used the criteria of the International Diabetic Federation. Preparation “Stilbene concentrate” (Institute “Magarach”, RF), obtained by water-alcohol extraction of Vitis vinifera grapevine, was used for correction. The research methods were used: somatometric, enzyme-linked immunosorbent assay - TLR-4, C-reactive protein (CRP), methods of general morphology, statistical analysis. Results. The use of high polyphenol drugs (“Stilbene Concentrate”) prevents the development of MS symptoms by exerting a hypoglycemic, lipid-lowering, and also anti-flammatory effect, which can be used for treatment and prevention of MS.