DEVELOPMENT OF TECHNOLOGICAL METHODS OF INCREASING THE VOLUME OF THE VACCINE RAW MATERIALS ON THE BASIS OF THE ALLANTOIC-AMNIOTIC FLUID OF CHICKENS EMBRYOS
Restoring domestic production of vaccines for humans and animals and reducing the share of imports is an important contribution to national security. Developing embryos of chickens and their extraembryonic liquid (allantois and amniotic) serve as raw materials for industrial cultivation for the production of viral vaccines for humans and animals. On the basis of their studies on hens specialized line of Russian white breed from "Genetic collections of rare and endangered chicken breeds" VNIIGRZE1 it was found that the increase in the yield of vaccine raw materials from developing embryos of chickens probably due to the use of technological methods, in particular, by reducing moisture loss of eggs to less than 6% for 12,5-day-old embryos and to less than 7% for 13.5 day-old embryos. When comparing different approaches (creating layer on the surface of the shell, isolation of certain areas of the shell, changing the moisture regime of incubation), the most effective method should be recognized with an increase of up to 65% relative humidity in the incubation medium in the period from 7 to 13 days. This makes it possible to increase the volume of allantois-amniotic fluid 11.7% in absolute measurement and 9.8% in relative (to egg weight).