APPLICATION OF CHITINOLYTIC ENZYMES FROM PAECILOMYCES MARQUANDII AND BROMELINE FOR HYDROLYSIS OF HIGH-MOLECULAR-WEIGHT CHITOSAN
Possibility was demonstrated and conditions were optimized for degradation of high-molecular-weight crab chitosan by chitinolytic enzymes of mycelial fungus <i>P. marquandii</i> and by plant proteolytic enzymes of Bromeline preparation. It was found that optimal conditions for chitosan enzyme lysis by chitinolytic preparation of <i>P. marquandii</i> were created at pH 5.0 and temperature 37 °C, by Bromeline preparation - at pH 4.6-5.0 and temperature 50 °C. Duration of chitosan enzymatic lysis by fungal chitinolytic enzymes equals 0.5h, by plant proteolytic enzymes - 2h.