MORPHOMETRIC CHARACTERISTIC OF CHRONIC TOXICITY ON FREQUENT REPEATED EXPERIMENTAL ADMINISTRATION OF AMIDES GROUP LOCAL ANESTHETICS
Objectives. To study chronic toxicity of local anesthetics (LA) of the amides group in the experiment, to determine the effectiveness of electroreflexotherapy for the prevention of chronic toxic reaction. Material and Methods. The experiment was carried out on 9 series of laboratory mice which were given submandibular or intraperitoneal injections of LA every 3-4 days 5 times. Then we performed the morphometric examination of the parenchymal organs. We compared the series: with submandibular injection of 2% lidocaine hydrochloride solution (series 1.1, 1.3), intraperitoneally (series 1.2, 1.4); with submandibular injection of 4% articaine hydrochloride solution (series 2.1, 2.3), intraperitoneally (series 2.2, 2.4). Series 3 (control) got submandibular injections of water for injections. Series 1.3, 1.4, 2.3, 2.4 had 5 procedures of electroreflexotherapy prior to the introduction of the LA. Results. According to the morphometry data, in series 1.1, 2.1 we revealed significantly more small, moderate necroses, inflammatory changes in portal tracts, cases of cholestasis, cell polymorphism that was not found in series 1.2, 2.2, 3. The cells of the lymphoid range dominated among the cells of inflammatory infiltrate. In series 1.3, 2.3 there were significantly fewer small, medium necroses, inflammatory changes in portal tracts, cases of cholestasis, there were no big necroses. A comparative evaluation of morphological changes of series 1.2, 2.2 and 1.4, 2.4 revealed a statistically significant decrease in the number of small necroses. Conclusions. The results indicate the influence of the LA injection zone on the development of toxic reactions and prove that when injecting LA in the head and neck area, electroreflexotherapy is able to provide reliable hepatoprotective effect.