THE USE OF ATOMIC FORCE MICROSCOPY IN ONCOCYTOLOGY FOR DETERMINATION OF TREATMENT TACTICS
The use of atomic force microscopy (AFM) affords new opportunities in cytological diagnostics, because this method allows to get image of cell surface with resolution of several nanometers during short period of time (minutes). The objective - to evaluate the role of AFM in cytological diagnosis of tumors and tumor-like processes. Materials and methods. We use study complex «Integra» by NT-MDT, Zelenograd, Russia. The probe nanoscale laboratory «Integra» includes a complex of high-technology device based on AFM. 51 cytological specimen of breast and thyroid tumor, obtained by ultrasound-guided fine needle aspiration and scrapes of operative material were studied. There were 17 scrapes from surface of cervix. All specimens were studied with conventional light microscopy and then with AFM. Scanning was performed in tapping mode. Obtained data on microrelief of cells and on its local properties were processed by optional software included in Integra software. Conclusion. New method of AFM allows to determine objective differential and diagnostic criteria for adenogenic and squamous cell cancer. For breast and cervical cancer the difference between height of nucleus and cytoplasm was detected. The objective criteria for differential diagnosis of papillary thyroid cancer and follicular adenoma may be the ratio between height of nucleolus and nucleus. AFM method detects viral changes in cells, one aspect of which is perinuclear cytoplasmic clearing. AFM allows to evaluate the results of immunocytochemical reactions. In our study for AFM Her2/neu hyperexpression was represented as well-defined closed prominence of cytoplasm.