THE STUDY OF THE MICROBIOME SPINY STURGEON GROWN UP UNDER CLOSED WATER SUPPLY WITH THE METAGENOMIC METHODS USED
The aim of the research is to analyse the microbiome of the skin, gill plates and intestines of the sturgeons. When having the structure of the microbial family of spines bred under closed water supply (CWS) investigated, we can make a conclusion for both - physiological condition of Àsh, and potential pathologies. To compile a collection of samples, the latter being taken from 10 spine species (by 5 pieces from the two let-in basins №3 and №6). To study the microÁora of the skin surface some pieces of the Àns, respiratory organs pieces of the gill plates, and to explore the natural microÁora of the digestive system - swabs from rectum by inserting swab through the anus were taken. The samples were placed in 96% ethyl alcohol at the collection sites. Each sample was assigned an identiÀcation number. DNA from the tissue of sturgeon were isolated using the kit reagents (MACHEREY-NAGEL NucleoSpin Soil) of the company MACHEREY-NAGEL (Germany). Processing of the sequences obtained had been performed with the use of Trimmomatic and Fastq-Join utilities, OTU-picking had been performed using the QIIME package. In the course of studies it had been found that the greatest differences between the basins have microbiomes of the intestinal opening, and the smallest - microbiomes of the Àn surfaces, which means that the degree of inÁuence of the basin on the identiÀcation of differences between the microbiomes has increased in the following series: Àn - gill - intestinal families.