Optimization of Culturing of Euraryotic Producing Cells based on CHO Line for Obtaining of Biopharmaceutical Preparations
A current review describes the aspects of the Chinese hamster ovary cells (CHO) cultivation as a major system for the production of recombinant protein biopharmaceuticals. A comparison of various types and regimes of culturing was performed. A special attention was paid to the optimization of culturing conditions aimed at the enhancement of productivity and protein glycosylation. The selection of physicochemical parameters (temperature, pH, dissolved oxygen) and composition of nutrient medium, additives and supplementats were discussed. A comparative revewing of different bioreactor types was performed.