INFLUENCE OF "TSITOVIRA-3" ON THE EXPRESSION OF IL-8 AND ICAM-1MONONUCLEAR LEUKOCYTES OF PERIPHERAL BLOOD OF THE PERSON AND CAGES OF THE EA.HY926 INVITRO LINE
The aim of the current study was to investigate cell mechanisms by means of which the "Cytovir-3", the drug for prevention and treatment of the influenza and acute respiratory viral infection, exerts its pharmacological effects. As a cellular model, an endothelial EA.hy926 as well as mononuclear leukocytes (ML) of human peripheral blood were chosen. The effects of co-incubation of "Cytovir-3" and cell lines on IL-8 and ICAM-1 expression were evaluated. In the presence of "Cytovir-3" at concentration of 10.0 ng/ml and 1.0 ng/ml, we found a significant increase in spontaneous secretion of IL-8 by endothelial cells by 10 % and 15 % accordingly. Besides, at concentration of 10.0 ng/ml the investigating drug significantly enhanced the expression level of ICAM-1 induced by TNFa. Revealed stimulating action of "Cytovir-3" on endothelial cells and ML can suggest its influence on vascular component of inflammatory reaction.