CHARACTERISTICS OF SKIN HORMONAL BALANCE REGULATION IN FEMALE MICE IN DYNAMICS OF TRANSPLANTABLE B16/F10 MELANOMA GROWTH
Background: Skin cells are capable of the local synthesis of sex and regulatory hormones which makes it an analogue of the hypothalamic-pituitary-gonadal system and facilitates independent melanoma hormonogenesis. Aims: to study the level of hormones in the tumor, perifocal zone, the skin is unaffected, and the pituitary gland in the dynamics of growth of melanoma B16/F10 in female C57BL/6 mice. Materials and methods: Changes in the local steroidogenesis in the dynamics of the growth of transplanted В16/Р10 melanoma (from week 1 to week 4) were studied in 40 female С57BL/6 mice. Hormones were determined by standard ELISA methods and radioimmunoassay in homogenates of tumor, surrounding tissues and intact tissues. Results: Tumor and then surrounding tissues showed an increase in estrogens, mostly due to estrone increase, with the development of relative androgen deficiency primarily in tumor and then in the perifocal zone and in intact tissues. Conclusions: Regulation and stimulation of such changes were performed at early stages by autocrine regulatory mechanisms and cells of primary and growing melanoma, and later by the paracrine method spreading its influence on surrounding tissues and the whole body.