CALORY RESTRICTION ALTERS THE ACTIVITY OF ANTIOXIDATIVE SYSTEM ENZYMES AT THE MICE WITH MELANOMA B-16
The aim of this study was to investigate the influence of calory restriction on the development of experimental tumor melanomaB-16 at mice. In the investigation female С57/BL6 mice divided into four groups - control animals kept a basic diet and calory restrictive diet, the animals with melanoma B-16 kept a basic diet and calory restrictive diet were used. Liver supernatant was used for spectrophotometric assessment of malonic dialdehyde level, the activity of antioxidative enzymes such as superoxide dismutase, catalase, glutathione peroxidase and glutathione S-transferase. Expression level analysis of sirt1 in tumor tissue was performed by RT-qPCR. Calory restriction of melanoma B-16 mice was not found to activate lipid peroxidation and to alter the activity of superoxide dismutase and catalase. At the same time, glutathione peroxidase activity and glutathione S-transferase were decreased, accordingly, 1,6 and 1,2 times. Under this, sirt1 expression level in tumor cells of calory restrictive mice was increased. This effect was absent at mice kept a basic diet. The findings allow to suppose that calory restriction influences on tumor development by means of alterations of antioxidative enzymes activity and sirt1 expression level.