ИЯВЛЕННЯ КОРІВ РЕЗИСТЕНТНИХ АБО ЧУТЛИВИХ ДО МАСТИТІВ НА ОСНОВІ ЛІМФОЦИТАРНИХ І ДНК-МАРКЕРІВ ГОЛОВНОГО КОМПЛЕКСУ ГІСТОСУМІСНОСТІ
The article describes the use of antigens class I major histocompatibility complex genes and alleles BoLA-DRB3 as immunogenetic markers of susceptibility and resistance of cattle to disease of the udder. Genetic condition of specific resistance of animals to disease resistance and high overall immunoreactivity is established fact and is mostly associated with the major histocompatibility complex genes or MOE (Major histocompatibility complex), which is present in all vertebrates and provides an immune response to foreign antigens. In the literature there is a sufficiently large number of reports related to the association BoLA and mastitis. The study was conducted on cows Ukrainian black and white dairy cattle. To identify the "information " class 1 antigens and gene alleles DRB3 BoLA- base system was formed from a group of resistant and susceptible to diseases of the udder of cows. Identification of class I antigen BoLA- held system standard two-stage test for lymphocytotoxic Kissmeyer-Nielsen as modified for cattle. The range of alleles BoLA-DRB3 gene was studied by PCR. Comprehensive analysis of "information " and alleles BoLA antigen -DRB3, identified in the population, and the results statusmetriya and biometric analysis, the BoLA- antigens and alleles BoLA-DRB3 associated with mastitis, which can be proposed as markers associated with susceptibility and resistance to disease. To lymphocyte antigens are markers BoLA-A A6 and A17, which are associated with resistance and antigen W2, W6, W15, W19, A3 and W31, which affect the susceptibility of cows to mastitis. By DNA markers are gene alleles BoLA-DRB3: with resistance to mastitis associated alleles * 13 and * 22, and a close relationship with susceptibility to inflammation of the udder of cows showing allele BoLA-DRB3.2 * 24 and * 26.