Experimental Studies Cross Immune Response to Antigens of the Virus Strains of Tick-Borne Encephalitis Different Genotypes in BALB/c Mice, Immunized with Various Embodiments of Tick-Borne Encephalitis Vaccine
Were studied in experimental researches for characterizing the cross immune response of BALB/c-mices, immunized with various embodiments of tick-borne encephalitis vaccines, against TBE virus strains of the Far Eastern, European and Siberian genotypes. The need for this research stems from the fact, that vaccines are prepared using TBE strains belonging to two genotypes of TBE virus: Far East (domestic TBE vaccines) and European (foreign TBE vaccines). Early was shown in the evaluation of immunogenicity on BALB/c-mices, that domestic and foreign TBE vaccines are quite active against strains of the Far Eastern and European TBE virus genotype. In recent years, in the circulation of TBE virus in natural foci of infection dominates the Siberian genotype TBE virus. For the study of humoral immunity response by vaccination against TBE used HAI (GA-antigens to four strains of different genotypes of TBE virus) and ELISA (two different sets of reagents for the detection of antibodies to the Far East or European genotypes of TBE virus). In HAI and ELISA confirmed the presence of cross-immune response to domestic and foreign TBE vaccines to different genotype TBE virus antigens. It is shown that in terms of seroconversion and geometric mean HAI antibody titer vaccine Entsepur, Germany, inferior to other vaccines, with significantly higher rates revealed to the homologous vaccine this European TBE virus antigen than the antigen Siberian genotype, and especially to the Far East antigen genotype. In ELISA set high values of the geometric mean antibody titer and the total rate of seroconversion to antigens of the east and west of the TBE virus genotypes for all variants of TBE vaccine - domestic and foreign.