INFLUENCE OF FIBROBLAST SECRETORY PRODUCTS ON OXYGEN-DEPENDENT NEUTROPHILE METABOLISM
The influence of soluble secretory factors of dermal fibroblasts of many days cultivation on the respiratory burst of primed and non-primed human neutrophiles in luminol-dependent chemiluminescence was studied. Fibroblast supernatants had no influence on spontaneous chemiluminescence of neutrophiles. Supernatants of fibroblasts inhibited the reactivity of neutrophiles primed with opsonized zymosan. It was probably bound with reserve exhaustion of neutrophiles and their inability to response to additional stimulus. This reaction can be a negative effect of secretory fibroblast products that are destroying the defending properties of neutrophiles. From the properties of whole organism this situation could lead to dilation of pathological process. Neutrophile induction with fibroblast supernatants with subsequent addition of opsonised zymosan has no significant effect on the neutrophile chemiluminescence. Their were no differences in fibroblast supernatants influences on neutrophiles respiratory burst produced by healthy children and children with Crohn's disease. Significant differences were shown in fibroblast secretion of cytokines and other biologically active factors. Supernatants had significantly reduced levels of TNF-α, IL-6, IL-10 and collagen IV against of the high levels of FAP and fibronectin.