Identification of 2-acetyloxybenzoic acid in blood plasma
The features of the 2-acetylhydroxybenzoic acid identification in blood plasma have been studied. An acetone has been suggested as an isolating agent for extraction of 2-acetylhydroxybenzoic acid from biological material. The possibility of purifying the analyte from endogenous substances by the adsorptive chromatography in a silica gel "L 40/100 microns" column using a mobile phase of hexane and diethyl ether (5:5) has been shown. To identify and quantify 2-acetylhydroxybenzoic acid in extractions from biomaterials we have suggested the methods of thin-layer chromatography of silica gel, ultraviolet and infrared spectrophotometry and GLC combined with mass selective detection. The technique of chemical and toxicological studies of 2-acetylhydroxybenzoic acid in blood plasma, which allows to identify 88.19-89.23 ± 2.29-3.56% of the compound with its proportion of 2.5-50.0 mg in 25g biofluid, has been developed.