Genotypical variability of the structure of gene coding pertactin, strains of Bordetella pertussis discovered in Russia
Research objective - analysis of the variability of structure of the gene pertactin, the strains of B. pertussis circulating in Russia in 1948 - 2014. Materials and methods. In work were studied 412 strains of B. pertussis isolated from patients with whooping cough. Genotyping of strains were carried out by two-stage PCR and a sequencing. Results. Circulation of five (prn1, prn2, prn3, prn4, prn9) alleles was established and analysis of changes in the structure of the prn gene of the strains of B.pertussis in dynamics is carried out. Consecutive change of the strains with "vaccine" prn1was shown to alleles on the strains with "non-vaccine" (prn2, prn3, prn4, prn9) alleles.