Вплив епіхлоргідрину на слизову оболонку шлунка щурів
Research of changes of some stomach mucous membrane indexes of rats under epichlorohydrin action were conducted. It is set that the action of ECH resulted in diminishing of height of gastric glands proper of mucous membrane of rats`stomach, amount of ephithelial cells and chief cells in one gland, which are kept during sixty days after finishing of ECH action. Introduction. The organs of the digestive system are characterized by the high level of sensitiveness to influence of exogenous factors. Morphological and functional changes in a stomach - one of basic organs of the digestive system - frequently are results of influence of factors with chemical or physical nature on an organism. Influence of chemical agents on the stomach structure is studied not enough. So, the study of this problem presents particular scientific interest. Epoxides are one of the most famous and widely used classes of reactive mixtures. Studies have shown that the effect of epoxy resins is the arising of diseases of the cardiovascular and digestive systems. One of the most toxic components of epoxy resins is epichlorohydrin (ECH), that is used in the production of rubber, solvents and pesticides. Research purpose: To study influence of epichlorohydrin on the height of gastric glands proper, amount of gastric glands proper ephithelial cells and chief cells of mucous membrane of rats` stomach fundus. Materials and methods: Experiment was conducted on mature outbred white rats-males, which were divided into 2 experimental groups. The first group was made by intact rats, second by rats, exposed to influence of ECH. The fence of material was produced on the first, seventh, fifteenth, thirtieth and sixtieth days after completion of ECH action. The morphometric analysis of mucous membrane and submucous layer of rats` stomach fundus was produced at lightoptical level after the preliminary colouring of cuts with hematoxylin-eosin. Digital pictures were processed by program "Morpholog". Research results and their discussion. At the analysis of changes of height of own glands of rats` stomach fundus mucous membrane it was set after completion of action of ECH, that on the first day of research this index was less than for control group rats on 43,1 % (р<0,001). On seventh day after completion of ECH influence diminishing was 28,7 %, on the fifteenth day - 44,1 %, on the thirtieth - 20,5 %, on the sixtieth - 15,7 %. The action of ECH resulted in diminishing of ephithelial cells amount in one gastric gland proper of rats` stomach fundus. On the first day after completion of ECH introduction the amount of cells diminished on 39,1 %, on the seventh - on 28,1 %, on the fifteenth - on 44,5 %, on the thirtieth - on 19,5 %, and on the sixtieth day of research - on 14,4 % (р<0,01). In all terms of research influence of ECH results in changes of chief cells amount in one gastric glands proper of rats` stomach fundus. On the first day after finishing of ECH action diminishing of this index was 41,7 %, on the seventh - 36,6 %, on the fifteenth - 45,7 %, on the thirtieth - 23,6 % (р<0,001), and on sixtieth day - 17,7 % (р<0,01). Conclusions. 1) Influence of ECH results in diminishing of gastric glands proper height of rats` stomach fundus, amount of ephithelial cells and chief cells after completion of ECH action. 2) Degree of diminishing of gastric glands proper height of mucous membrane, amount of ephithelial cells and chief cells in one gastric glands proper during sixtie days fall. Further researches of conformities will let us to get more detailed idea about the mechanisms of action of ECH on the stomach structure.