Extracellular Phosphomannan as Phospate Reserve in Yeast Kuraishia capsulata
T was found that extracellular phosphomannan is the main phosphate reserve in the yeast Kuraishia capsulata, unlike other yeast species effectively absorbing P <sub>i</sub>. Under nitrogen starvation, K. capsulata absorbs essentially all the P <sub>i</sub> from medium containing 240 mM glucose, 2,5 mM Mg <sup>2</sup>+, and 11 mM KH <sub>2</sub>PO <sub>4</sub>. Inorganic polyphosphate level in the cells was about 14% of the P <sub>i</sub> absorbed. The main part of P <sub>i</sub> (~ 60%) was found in the fraction of extracellular phospho-mannan that can be used as a carbon and phosphorus source by this yeast in deficient media.