DEVELOPMENT OF A TECHNIQUE FOR MICROSATELLITE ANALYSIS OF PEARS (<i>PYRUS</i>) FROM THE RRIFCB COLLECTION
Microsatellite DNA markers have a wide range of applications in fruit crop breeding. For example, for the study of genetic diversity, the construction of molecular genetic maps, for the genotyping of plant material, the identification and selection of genomic regions containing loci of economically important traits. We have completed the work on the development of the method of microsatellite analysis of pears from the RRIFCB collection. A selection of microsatellite loci for simultaneous PCR analysis was carried out. Amplification techniques for six microsatellite loci (CH01f07a, EMPc11, CH01d08, EMPc117, CH03d12, CH04e03) have been developed. We also obtained data on the polymorphism of these loci on 9 pear cultivars (Osennyaya zholtaya, Pervomaiskaya, Pin Go Li, Ploschanskaya, Podgoryanka, Polveria, Striiskaya, Tem bo Li and Yakovlevskaya). Polymorphism was found in all loci. Further, the developed methods can be used to study the polymorphism of pear genotypes. For four loci (CH05c06, GD147, CH01d09, CH03g07), it is necessary to experimentally select the annealing temperatures.