Development of Immunoenzyme Assay for Detection of Soybean Raw Materialin Food Products
An enzyme-linked immunosorbent assay (ELISA) has been developed to control the presence and estimate the content of soybean raw materials in meat food products, based on the binding of specific antibodies to antigenic determinants of a soybean trypsin inhibitor. Due to the possible influence of enzymatic and high-temperature treatments of products that can disrupt the native structure of the inhibitor and lead to its fragmentation, the analysis is based on competitive interaction with antibodies of the antigen contained in the sample and immobilized in microplate wells. The conditions to implement the ELISA were established, providing a minimum detection limit and high accuracy of measurements. The developed assay was characterized by duration of 60 min, detection limit of 3.5 ng/mL and range of detectable concentrations of 7.7–110 ng/mL. The possibility of using the proposed methodology for testing meat products was shown.