INCREASING THE PLANT REGENERATION FREQUENCY IN THE CALLUS CULTURE OF STRAWBERRY AND ANALYSIS OF THEIR VARIABILITY
Cultivation of callus tissue with the subsequent receiving of regenerants is a new direction in a breeding research. It is necessary to develop the technology of reliable receiving of regenerated plants for successful carrying out of research with use the callus cultures of wild strawberry. This method will open the ways of high effective mass breeding in vitro culture. The work’s purpose is the development of methodical aspects of increase of regeneration frequency in vitro system and the analysis of variability of plants from callus culture of wild strawberry. The growth regulators of cytokinin, auxin and gibberellic nature are used as inductors of a stem organogenesis. The results of research on regeneration of plants in the callus culture of wild strawberry (callus age is from 30 to 160 days) are given. It is established that the frequency of shoot regeneration was from 6,7 to 64% and depended on present of growth regulators in the medium, their concentration and an initial genotype. Some variants of induction medium on the basis of Murashige-Skoog recipe with an optimum combination of growth regulators are developed. The effective two-stage method of increase of regeneration ability of callus tissue is developed. The analysis of regenerated plants showed the considerable degree of variability of some traits which remained in R1 and R2 vegetative generations. The developed methodical approaches to increase of morphogenetic potential of callus cultures can be used for other varieties relating to a type of F. ananassa Duch. for increase of efficiency and direction of wild strawberry breeding process.