Effect of pyrimidine-4-1(H) -OH derivativeon vasodilating function of vascular endothelium of rats under conditions of experimental traumatic brain injury
Background. In view of the steady increase in cases of craniocerebral trauma and a significant role in the pathogenesis of craniocerebral trauma of endothelial dysfunction, preservation of endothelial function is of undoubted scientific and practical interest. The aim of the study. To evaluate the effect of the new peptide-substituted pyrimidine-4-1(H) derivative on the vasodilating vascular endothelial function in rats under experimental traumatic brain injury. Materials and methods. The experiment was performed on 48 male rats of the Wistar line. Traumatic brain injury was reproduced by the method of free fall of a weight of 150 g on the parieto-occipital region of the skull of the animal from the height of 50 cm the test compound and the reference drug (gliatilin) was administered for 3 days from the moment of playback of traumatic brain injury. On the 4th day, the state of vasodilating vascular endothelial function was assessed by changing the speed of cerebral blood flow during modification of no synthesis. Additionally, the concentration of homocysteine in serum was determined. Results and discussion. As a result, it was found that the use of a new peptide-substituted derivative of pyrimidine-4-1(H)-OH at a dose of 50 mg/kg contributed to the preservation of vasodilating vascular endothelial function in rats, as may be evidenced by the restoration of vascular reaction in response to the introduction of acetylcholine and nitro-L-arginine methyl ether, as well as a smaller manifestation of the phenomenon of "L-arginine paradox". In addition, the use of the studied compound contributed to a decrease in the concentration of homocysteine in serum by 34.8% (p<0.05) compared with animals that did not receive pharmacological support. Conclusion. Thus, according to the results of the study, it is possible to assume the presence of the studied peptide-substituted derivative of pyrimidine-4-1(H)-it endotheliotropic activity comparable to the effect of the reference drug - gliatilin.