Modified Chemiluminescent Method for Determination of Antioxidant Capacity of Biological Fluids and Tissues
In this study, we presented the modified method for determination of antioxidant capacity of biological fluids and tissues based on the use of a chemiluminescent model system in which oxidation of luminol was induced by 2,2′-azobis(2-amidinopropane)dihydrochloride. It is shown that the addition of EDTA as an extra component gives the system a number of advantages. The intensity of chemiluminescence at the stationary stage is increased substantially (by 6-7 times), stationarity remains unchanged for a long time (more than one hour). Besides, it should be noted that no significant increase in the intensity of chemiluminescence occurred in the presence of biological fluids or individual antioxidants (human serum albumin, ascorbic acid) after the end of the latent period but it was observed in the absence of EDTA. Taking into account that the values of latent periods, which were determined while introducing some low molecular weight antioxidants (trolox, uric acid), as well as human biological f luids (blood serum, tear fluid) into our system, were not significantly different from those obtained in the system without EDTA, we suppose that EDTA has no impact on luminol oxidation reactions. The proposed system can also be used to evaluate antioxidant capacity of various tissues. The obtained data are discussed in terms of prospects for the use of the proposed modified method for determination of antioxidant capacity of biological material in clinical practice.