THE NEW APPROACHES TO PREVENTIVE CANCER IN HYPER- ENDEMIC NIDUS OF OPISTHORCHOSIS
68 % of West-Siberian geographically-industrial region' population suffer from opisthorchosis, 96.6 %of helminthiasis displayed the superinvasive form. We have determined the involvement of opisthorchosic invasion in liver cancer development. In our in vivo animal models superinvasive opisthorchosis gave rise the mutagenesis of K-ras, p53, K-raf, B-raf, C-kit, p16 and their combinations, proliferation of ductal epithelium and oval cells. We have also detected the mutation of p53 (100 %) and K-ras and K-raf (50 %) in liver tissue' of patients died from primary cholangiocellular carcinoma. Based on our observations we conclude that target and immunomodulating therapy of patients with superinvasive opisthorchosis normalize the genetic apparatus: 3 months of treatment reduce the mutation of p53, K-ras, B-raf and p16 up to 80 %, 6 months of treatment - 84.4 %, 12 months - 85.2 % and 24 months - 92.2 %.