ANALYSIS FEATURES OF ORGANOPHOPHORUS NERVE AGENTS REACTIVATED FROM BLOOD PROTEIN ADDUCTS WHILE IDENTIFYING EXPOSURE TO CHEMICAL WEAPONS
The detection of nerve agents or their fluorine derivatives, reactivated from phosphorylated adducts in blood (plasma, serum) with blood proteins using fluoride ion action, is a commonly recognized method to identify human exposure to nerve agents. In the present work, a highly sensitive detection of sarin and soman, as well as fluorine derivative of Russian VX (RVX-G) was obtained by optimization of sample preparation procedures and use of gas chromatography with high selective detectors. Solid-phase micro extraction was successfully used in the soman analysis. Solid-phase extraction with a highly cross-linked styrene and methacrylate copolymers was found effective in the analysis of soman, sarin, and RVX-G. Advantages of tandem mass spectrometry and high-resolution mass spectrometry in the detection of nerve agents and their fluorine derivatives reactivated from blood protein adducts were demonstrated.