Changes in hemostatic characteristics of the clot after storage of platelet concentrates prepared by different methods
The hemostatic characteristics of platelets were tested during various periods of storage of platelet concentrates (PC) prepared by different methods. Twenty-six PC specimens prepared on 100% autologous plasma (PC-P) and 24 specimens prepared with the use of 70% plasma substituting solution (PC-PSS) were studied. The testing was carried out on the day of PC preparation, after 24 h, and on days 3 and 5 of storage. The activity of platelet granule reactions and of interactions between platelets reduced with storage in PC-P and PC-PSS. Activated platelets could no longer modify the characteristics of the clot forming in PC-P by day 3 of storage. The quality of the clot generally deteriorated during storage, its elasticity reducing. In the PC-PSS the activated platelets could not modify the clot throughout the entire period of storage. The quality of the clot in these samples gradually deteriorated from the day of preparation to day 3, its elastic and deformation characteristics reduced. It is advisable that the duration of PC storage be no longer than 5 days, irrespective of the method of their preparation.