EFFECT OF AMINO ACID ON THE DEVELOPMENT SKIN TISSUE ORGANOTYPIC CULTURE
The effect of the 20 coded amino acids was investigated on the development of the processes of the proliferation in the organotypic tissue culture of rat skin. The amino acids lysine, arginine and glutamine acid at 0,05 ng/ml concentration stimulated the cellular proliferation in the growth zone of explants by 29-30%. The mustard-like agent cyclophosphane at 1 mg/ml concentration inhibited the cellular proliferation. The delay of this inhibiting effect of cyclophosphane was observed by the combined action of stimulating amino acids with the cyclophosphane. Thus, the amino acids with the charge radicals can be protectors of the cellular proliferation by the toxic effect of the cyclophosphane on the skin. This effect can be used for the treatment of the mustard injury of skin and for the delay of the adverse effect of cytostatic in oncology (1 figure, 1 table, bibliography: 28 refs).