Association of polymorphic variants of the MSTN gene with body measurements in Saanen goats
Myostatin has a pleiotropic effect and plays an important role in the regulation of muscle tissue growth, lipid metabolism and bone development in mammals. The aim of our work was to evaluate the relationship between body measurements in mature goats of the Saanen breed in connection with variants of 5 bp indel polymorphism. the MSTN gene. The sample included 43 Saanen goats aged 3-5 years, belonging to CJSC PZ Prinevskoe (Leningrad region). Animals were genotyped by PCR-RFLP to detect indel polymorphism in the MSTN gene caused by a 5 bp insertion. in the 5’UTR region. The collection of phenotypic data was carried out once, before the start of the breeding season (July). Such measurements were measured as height at the withers (cm), height at the sacrum (cm), oblique body length (cm), chest depth (cm), chest width (cm), chest girth (cm), metacarpus girth (cm). Based on the data of body measurements, the main index of body built (%) were calculated. The results of the study showed that the frequency of genotypes in the studied group of goats was as follows: BB - 0.209, - AA - 0.302 and AB - 0.488. The occurrence of alleles A and B of the MSTN gene was 0.574 and 0.453, respectively. On average for the sample, the data obtained from body measurements corresponded to the parameters desired for Saanen goats. An analysis of the results of our work did not reveal significant associations of different genotypes for the 5 bp indel polymorphism. in the 5’UTR region of the MSTN gene with body measurements and index of body built in mature goats.