EFFECTIVENESS OF DIMETHYLSULPHOXIDE ON BIOFILM FORMATION BY STAPHYLOCOCCUS AUREUS STRAINS, AS THE INDICATOR OF RECOVERY REACTIONS FOR PURULENT-INFLAMMATORY PROCESSES
One of the most urgent issues of microbiology and treatment of purulent diseases is the reducing structure and function in the formation of pathological processes of a purulent-inflammatory nature. This is especially important in connection with the increase in the number of immunoresistantindividuals and the formation biofilms by microorganisms with the participation of opportunistic strains. One of the most common pathogens of purulent inflammatory diseases is Staphylococcus aureus, the formation biofilms with strains of which leads to the appearance of antibiotic resistance, slowing down the recovery processes and the formation of complications. In connection with this urgent issue remains the study of drugs that can enhance the duration of action and the therapeutic effect of the treatment used for purulent-inflammatory diseases. To achieve this goal, it is possible to use dimethyl sulfoxide, due to its antiadhesive effect, as well as the lack of information on the formation of resistance and biofilm formation under its influence. The study carried out on 20 strains of bacteria of the genus Staphylococcus isolated from the wounds of patients. The solution of "Dimeksid" applied in three different concentrations. The formation of biofilms by microorganisms carried out by photometry method, determining the possibility of adhesion of microorganisms on the surface of a 96-hole polystyrene plate, followed by coloring of the preparations. During the statistical processing of the experimental data, a significant decrease in biofilm formation observed when a dimethylsulfoxide introduced into the medium, as well as a decrease in the adhesive activity of staphylococcs.