Method of combined treatment of acute subcutaneous purulent mastitis in a surgical experiment
The aim of the study is to experimentally substantiate the effectiveness of combined surgical treatment of ASPM in an experiment using LILR and nanodispersed zinc oxide (NZO) based on 0.24% intralipid solution. Materials and methods. In 32 laboratory animals, female rabbits were modeled with ASPM in the subalveolar region with the introduction of a modified two-light catheter through a puncture, the infected balloon of which was inflated with Staphylococcus aureus 209-P and E. E. Coli ATCC 25922 to 2.0 ml for a period of 5 days. For all animals the surgical stage and the subsequent ten-day treatment provided for the opening of the abscess, evacuation of the contents and removal of the intracavitary tissue sequester, followed by sanitation of the abscess cavity. The animals were divided into 4 groups: rabbits of the first group underwent sanitation of the ASPM cavity and the use of antibiotics; animals of the second group underwent sanitation of the cavity and the use of LILR; animals of the third group underwent sanitation of the cavity followed by local application of NZO; animals of the fourth group underwent sanitation in combination with the introduction of NZO and the use of LILR. Clinical, planimetric, instrumental, bacteriological and morphological methods were used for a comprehensive assessment of the effectiveness of the treatment of the purulent process. Results. Based on microbiological, radiological and ultrasound data, the best treatment results were obtained in the fourth group of animals, where it was possible to achieve the absence of microflora growth from the ASPM cavity by the 5th day, thereby reducing the phase of inflammation, accelerating the regeneration phase and reducing the volume of the cavity from 1.9 ± 0.1 cm3 to 0.25 ± 0.01 cm3. Almost complete obliteration of the ASPM cavity by the 10th day of treatment was achieved due to the trophico-regenerative, anti-inflammatory and immunomodulatory synergistic action of NZO and LILR.