Physiological mechanisms of laser correction of stress states in cattle
Low-intensity laser radiation has an integrated effect on the functioning of all body systems, normalizing their work in pathology. However, a General theory of physiotherapy has not yet been developed. It is obvious that the search for the acceptor of laser radiation is the most important in solving the problem of the action of low-intensity laser radiation. It is known that low-intensity laser radiation can have its effect on the body through the activation of stress-implementing systems. In this regard, the aim of the work is to study the mechanisms of action of low-intensity laser radiation on red b1ood ce11s of catt1e in the state of physiological norm and under technologica1 stress. In vitro experiments investigated the effect of low-intensity laser radiation with a wavelength of 830 nm, a total power of 90 mW on erythrocytes pre-incubated with adrena1ine, cortiso1 and p-adrenoceptor blocker-propranolol. Irradiated red blood cells continuously Autonomous laser shower "marsik" for 15 minutes. The concentration of ATP and 2,3-diphosphoglycerate (2,3-DPH) was determined in all groups of cells by nonenzymatic method. It was revea1ed that the effect of low-intensity laser radiation on the erythrocytes of animals not subjected to stress during incubation with adrenaline reduced the concentration of ATP, preincubation of cells with cortisol, propranolol caused, on the contrary, an increase in the concentration of ATP relative to the control cells. The effect of low-intensity laser radiation on the erythrocytes of anima1s after exposure to stress a1so caused a mu1tidirectional effect on the leve1 of ATP. Incubation of erythrocytes with adrenaline and propranolol led to an increase in the concentration of ATP under the action of low-intensity laser radiation, incubation of erythrocytes with cortisol s1ightly reduced the concentration of ATP. Under the action of low-intensity laser radiation on erythrocytes of animals not subjected to stress, there was a decrease in the content of 2,3-diphosphoglycerate after preincubation with the studied modifiers. Thus, low-intensity laser radiation can act as a modulator that provides homeostasis of cells depending on the functional state of the organism, which must be taken into account when deve1oping a technology of therapeutic action of low-intensity laser radiation.