DIRECT PROTEOMIC PROFILING OF HUMAN URINE AND BLOOD SERUM IN AN EXPERIMENT WITH 5-DAY DRY IMMERSION
Changes in proteome of urine and blood serum obtained from 14 healthy humans (age 21–29 yrs) medically certified for an experiment with dry immersion were analyzed. Urine and serum samples were pre-fractionated and enriched with magnetic particles MB-WCX and MB-HIC, respectively, on robot ClinProt (Bruker Daltonics) for direct mass-spectrometry profiling by MALDI-TOF. As a result, 143 protein peaks on the average were identified in urine samples. It was shown that a high variation coefficient in 23.7 % of protein peaks, i.e. double technical, points to the most plastic fraction of the urine proteome. In blood serum, 175 peaks were identified in a sample on the average. Comparison of baseline and immersion mass-spectra of the blood proteome revealed significant differences. Increased peak areas of several protein fragments – С 3 and С 4 fragments of complement system, high-molecular kininogen and fibrinogen – can be ascribed to human body adaptation to the experimental conditions.