SINGLE CELL PROTEOGENOMICS AS AN IMMEDIATE PROSPECT
Recently technical advances in genomic technology led to explosive growth of transcriptome-wide studies at the level of single cells. The review describes the beginning of single-cell proteomics which started soon after transcriptomic methods were developed. Thus, first studies have been published that used liquid chromatography-mass spectrometry to analyze shotgun proteomes of single cells. In these works, cells were separated by methods used in transcriptomics, e.g., by cell sorting, and mass-spectrometric analysis was performed by a modified method of tandem mass tags. Data integration of single cell transcriptomics and proteomics as a proteogenomic approach will provide better understanding of mechanisms of cell interactions in normal development and disease.