The using of synthetic oligopeptide for binding and removal of IL-6 from blood plasma
The article presents the results of an experimental study of the IL-6 interaction with the synthetic peptide Ser-Phe-Tyr-Arg, which is a structural analogue of the soluble IL-6 receptor (gp80). The interaction of the peptide with the cytokine was been evaluated by the change in the concentration of IL-6, which was assessed by enzyme immunoassay. The binding efficiency was been calculated based on a statistical analysis of the dependence of the concentration of the complex IL-6 with the peptide on the initial concentration of the cytokine. Studies have shown that Ser-Phe-Tyr-Arg, both in free and immobilized form, has the ability to bind IL-6. The results obtained are the basis for the development of medical products for hemosorption in order to extract IL-6 from human blood plasma.