Assessment of CD 64 expression on neutrophils as a biomarker of inflammation and an indicator of severity in septic patients
The level of the expression of CD64 on neutrophils by flow cytometry in 56 patients with sepsis was studied, the dependence of these parameters on the degree of severity of the condition on the scale APACHEII (in points), the severity of multiple organ dysfunction scale SOFA (in points), the content of the procalcitonin level (PCT) and leukocyte intoxication index (LII) was analysed. It was revealed that the level of MFI (MFI - mean fluorescence intensity) was significantly higher in patients 67,2 ± 21,6 compared with a group of healthy donors 24,1 ± 9,6 (significance of differences by Mann - Whitney test - p = 0,003). High - level of expression on neutrophils nCD64 directly correlated with more severe organ dysfunction (SOFA), theweight of the state (APACHEII) and a high content of procalcitonin in patients as well. These results may give the suggestionthat presence of CD64 on neutrophils may serve as a biomarker reflecting on the degree of systemic inflammation in sepsis, and may be a criterion in the prediction of the severity of disease and outcome in patients with sepsis. As a result of ROC - analysis, the optimal level of expression on neutrophils of CD64 as a predictor of the development ofsepsis in patients was 25,05 (MFI). With an increase in the expression of CD64 on peripheral blood neutrophils over 25,05 MFI (taken as a threshold value) AUC = 0.86 at rates the sensitivity of 71,43% (confidence interval (CI) 41,90% - 91,61%) and specificity of 89,66% (DI 72,65% - 97,81%).