A new approach to comprehensively assessing immunobiological responsiveness in a cohort to be vaccinated (revaccinated) against brucellosis
This paper describes a new approach to comprehensively assessing immunobiological responsiveness in a cohort to be vaccinated (revaccinated) against brucellosis, which is based on in vitro leukocyte antigen activation methods and flow cytometry technology. To estimate the intensity of post-vaccination antibrucella immunity, the authors recommend the use of a modified lymphocyte blast transformation reaction to detect blast cells by anti-CD34+ monoclonal antibodies, by further taking into account the results of the reaction using a flow cytometer. To identify hypersensitivity (allergization) to Brucella antigens, it is recommended to use the basophil activation test and a flow cytometric immunophenotyping test to estimate the expression of the degranulation marker CD63+.