Markers of activation expression on the blood lymphocytes in children vaccinated with Priorix
The expression of the activation surface markers (CD25, CD38, CD122, HLA-DR) was evaluated on the blood lymphocytes by flow cytometry in 25 children aged 1-2 years old, vaccinated with Priorix (group 1), and 24 children aged 6-7 years old, revaccinated with Priorix (group 2). The testing made before vaccine application, in a week and in 6 weeks after vaccination. In group 1 the significant elevation of the activated helpers (CD3<sup>+</sup>CD4<sup>+</sup>CD25<sup>+</sup>CD127<sup>hi</sup>) and late marker of activated T-lymphocytes (CD3<sup>+</sup>HLA-DR<sup>+</sup>) levels were founded in a week after vaccination. Expression of CD38 and CD122 markers was significant increased on the CD3<sup>+</sup>CD4<sup>+</sup> lymphocytes and decreased on the cytotoxic CD3<sup>+</sup>CD8<sup>+</sup> lymphocytes 1 week after vaccination. These characteristics were recovered to the normal ranges within a 6 weeks after the vaccine inoculation. In group 2 the significant elevation of the CD3<sup>+</sup>CD4<sup>+</sup>CD25<sup>+</sup>CD127<sup>hi</sup> lymphocytes and the reducing of CD122 expression on the CD3<sup>+</sup>CD4<sup>+</sup> lymphocytes were detected in a 1 week after vaccination. NK-cells activations was observed in a week after vaccination independently of the type (primary or secondary) of immune response. It is possible that such investigations can be informative for immune response on the vaccination monitoring in immunocompromised patients.