Physiological-biochemical characteristics of the use bull semen diluents on egg yolks and soya leсitine for cryoconservation
The physiological and biochemical characteristics of bull sperm by using diluents with chicken egg yolk and soy lecithin during the process of semen freezing were studied. It was revealed, that dilution of bull ejaculates with «Andromed» media showed better activity of mitochondrial respiratory chain links and oxidative enzymes and increased viability of sperm cells during incubation at 2–4 °C, compared to lactose-yolk-glycerol diluents. After cryopreservation with medium containing soybean lecithin, activity of thawed spermatozoa was higher by 11,3 % compared to diluent with egg yolk. The use of soy lecithin in the diluents increases activity sperm of thawed by 12,1 % after five hours of incubation, compared to medium with chicken egg yolk.