COMPARATIVE ANALYSIS OF ADMINISTRATION ASEPTIC IN CULTURE <i>IN VITRO</i> PLANTS <i>MELITTIS SARMATICA </i>KLOK
There were developed the methods of sterilization and introduction in vitro of a rare aromatic plants of the Belarusian flora of Melittis sarmatica Klok. The seeds and cuttings with axillary buds were used as a material for introduction in vitro culture. As the main sterilizing agents there were used the 0.1% solution of silver nitrate and the 0.1% solution of the diacid. Influence of a 6-benzilaminopurin (6-BAP), 24-epibrassinolid (EB), their combinations and concentration on in vitro viability and germination of plant seeds Melittis sarmatica in vitro is studied. The highest rates on sterility and viability of seeds were observed at a combination 6-BAP and EB in concentration on 0.5 mg/l (viability of 3-5%), at addition on medium only of 0.5-1.0 mg/l 6-BAP (viability of 2-6%), or only of 0.1-1.0 mg/l of EB (viability of 1-2%). The most high influence on sterilization (72.6%), regeneration (62.7%) and rooting (28.7%) of sterile cuttings have been received at seven-minute processing of 0.1% solution of the diacid.