Protein composition of blood serum of the same age grass carp affected by monogenea, before and after "Granulated brovermectin" and "Avesstim" use
The success of treatment fish with invasive diseases depends on availability of highly efficient antiparasitic drugs in the pharmaceutical market. Therefore, scientists are constantly working to develop anti-parasitic drugs for the treatment of fish that would have not only high efficiency, less toxic to the body and the cost of medical treatment, but also contribute to the normalization of metabolic processes. That is why the purpose of our researches was to study the effect of «Granulated brovermectin» and «Avesstim» on the protein composition of blood serum of carp fish infested by various ectoparasites. Researches were conducted in garden-fish farms, SOE «Fish farm Halytskyy» (now LLC «Fish farm «Burshtynskyy») Rohatyn raion, Ivano-Frankivsk oblast and FE «Dobrotvir fish factory» Kamianka-Buzka Raion, Lviv oblast, located on the warm waters of cooling ponds of Burshtynsk and Dobrotvir Thermal Power Stations. Fish weighing 45-47 g were selected for researches. It was established that the use of «Granulated brovermectin» and immunomodulator «Avesstim» caused activation of protein metabolism in fish body affected by pathogens Dactylogyrus lamellatus, Gyrodactylus ctenopharyngodonis, and mixed infestations and as a result there was indicated the increase of total protein, albumins, globulins and α-globulins in their blood serum. Thus, the drug «Granulated brovermectin» in the blood serum of affected by ectoparasites fish total protein content compared with the control, increased to 4.23 (P < 0.001), albumins - to 2.82 (P < 0.001), globulins - to 1.41 (P < 0.05) and α-globulins - on 1.23 g/l (P < 0.01). Combined use of these drugs showed the best normalizing effect on the infested fish: the content of total protein in blood serum, depending on the type of infestation, increased to 5.22-9.97, albumins - to 4.34-8.17, globulins - 0.88-1.45, α-globulins tо - 1.36-2.20 g/l, and these changes in all cases (the exception - the quantity of globulins in the blood of fish affected by gyrodactylus) were reliable (P < 0.05-0.001).