Leptin level and Q223R polymorphism of leptin receptor's gene in patients with abdominal obesity
Leptin levels were evaluated in patients with abdominal obesity and healthy subjects without obesity - all patients had different genotypes of leptin receptor gene. Leptin levels were evaluated by ELISA, Q223R leptin receptor gene polymorphism - by polymerase chain reaction (PCR). In patients with abdominal obesity leptin levels were higher than in healthy subjects (46.3 ±1.7ng/ml vs 23.5 ± 3.4 ng/ml; p = 0.0001). In obese females leptin levels were higher than in men with abdominal obesity (51.4 ± 1.8 ng/ml vs 29.7 ± 3.1 ng/ml; p<0.0001). Those subjects who had obesity since childhood had higher leptin concentration. Inverse correlation was seen between leptin and insulin levels and index of insulin resistance. QQ, RR and QR genotypes distribution did not vary between teenagers, healthy subjects and patients with abdominal obesity. Leptin level was significantly higher in RR genotype carriers than in QQ genotype carriers. In obese patients, RR genotype carriers, body mass index was higher than in QR and QQ genotypes carriers.