Molecular Cloning and Expression of a Gene that Encods a Human Leukemia Inhibitory Factor (LIF)
A gene for human leukemia inhibitory factor (LIF) has been cloned and expressed in E. coli BL21(DE3). A highly pure recombinant protein was obtained. It was shown that rhLIF with N-terminal HIS6 tract and hydrolysis site for thrombin is biologically active: it inhibits the differentiation of murine multipotent embryo trunkal cells.