Stability of the Human Endometrial Mesenchymal Stem Cells Karyotype In Vitro
The use of stem cells for therapeutic purposes presupposes the significant quantities and genetic stability of the transplanted cells. An increase in cell biomass is may only <i>in vitro</i>, which is associated with probable risks of their genetic stability violation. In this work, we analyzed the karyotypic stability of 3 endometrial MSC (eMSC) cell lines after the transfer of cells in vitro and during subsequent cultivation. We are showing the cells underwent karyotypic changes after transferred to in vitro system. The variability profile of analyzed eMSCs had an individual character. It was found the cells without gross chromosomal changes underwent a reversion to the karyotypic norm. Cells with chromosomal breaks and aneuploidy tended to further destabilize during cultivation. The obtained data make a significant contribution to the eMSC genetic study.