Change of the level of content of deformed Erythrocytes of the rats under the Influence of Phosphorus-Inorganic Intoxication
The article deals with the study of dependence between action of organophosphorus intoxication and number of deformed erythrocytes; the study was performed by means of scanning electronic microscopy with phosphacol. It is shown that the most active stage when organophosphorus intoxication has the biggest impact on erythrocytes falls on the first 15th minutes of its action. The study objective was to assess impact of organophosphorus intoxication on the number of deformed blood cells, and change in erythrocytes surface relief. White outbred rats were the objects of the research, males with body weight 180 - 200 g; 4 groups of animals. Animals of test group got intramuscular injections of phosphacol. Blood samples were taken from caudal vein. Data analysis showed that phosphacol had negative impact on the surface monolayer of erythrocyte membrane, causing decrease in deformability and transport characteristics of erythrocytes: in concentration of 0.5 LD it caused initial increase in volume with further apoptotic compression of cells; higher concentration of LD 50 caused decrease in cellular volume with the accompanying increase of cell rigidity. Affecting the blood of outwardly healthy rats with toxin helped to determine change of erythrocyte shape in vitro. Phosphacol caused echinocytosis: surface relief changed with the formation of crests, the destruction of membrane material was observed. It is connected with conformational changes of cytoskeleton and membrane structures, hemolysis rate slows down, and, consequently, deformability of cells also decreases. Configuration of erythrocytes can be observed in changing of physical and chemical properties of blood and metabolism of its cells. Deformation and aggregation of erythro cytes lead to disorders in microcirculation and rheological properties of blood. Disorders in the compensation and adaptive systems responsible for transport and detoxification body functions lead to the rise of deformed blood cells.