DIAGNOSTIC OPPORTUNITIES OF PROTEOMIC PROFILING IN GASTROENTEROLOGY
The modern stage of development of medicine is characterized by the appearance of hi-tech techniques, the most promising of which is the proteomic profiling, which allows to study complex cellular and inter-cellular communication, the primary structure of a protein and its posttranslational modifications. Technology platform for proteomic profiling presents two-dimensional electrophoresis in polyacrylamide gel (2-DPAGE), high performance liquid chromatography (HPLC/MS), time – of-flight mass spectrometry with laser-desorption ionization (MALDI-TOFF-MC), United by a common stages of the study. Proteomic analysis has been used in various fields of internal medicine, opening new diagnostic and prognostic capabilities. In gastroenterology research small, identified markers of acute pancreatitis, autoimmune hepatitis, examined the proteomic profile of patients with inflammatory bowel disease. For example, gastroesophageal reflux disease shows the importance of proteomic research at reflux esophagitis in different gradations and also complicated course of the disease.