MORPHOLOGICAL AND BIOCHEMICAL INDICATORS OF BLOOD OF FEMALE RATS F1 WHEN DRINKING DIFFERENT DOSES OF NANOGERMANIUM CITRATE
The article presents the results of studies of the effect of various concentrations of germanium citrate (GeC) on the organism of female rats F1 at the doses of 10 (group 1), 20 (group 2), and 200 (group 3) µg Ge / kg per body weight, which was obtained by the method of nanotechnology. A study of the biochemical and morphological properties of blood showed that in animals of all experimental groups the morphological parameters of blood did not have statistically significant differences from the blood parameters of the analogues of the control group, however, there was a tendency to decrease the number of red blood cells, increase the total hemoglobin and its average content in red blood cells, and hematocrit index as well the average volume of red blood cells. There was an increase in the number of leukocytes in the blood of female rats of the 1<sup>st</sup> group by 47,0%, and the 2<sup>nd</sup> - by 79,6% (p<0,05) and the 3<sup>rd</sup> - by 26,9% (p<0,05). In the 2<sup>nd</sup> group animals found to be a decrease number of lymphocytes by 6,0% (p<0,01), and the 3<sup>rd</sup> group showed an increase in neutrophils by 24,6% (p<0,05). In the blood of animals of the 1<sup>st</sup> and 2<sup>nd</sup> experimental groups, there was a tendency to increase the amount of iron in the relation to the control group, when used 200 µg Ge / kg b.w., a statistically significant double increase was revealed. The total iron-binding ability of blood serum in animals of the 1<sup>st</sup> experimental group decreased by 13,1% (p<0,05) and increased in animals of the 2<sup>nd</sup> experimental group by 10,6% (p<0,05) in the relation to the control group. In animals which used a high Ge concentration (200 µg Ge / kg b.w), this indicator was the same as in the control group animals. In animals of 1<sup>st</sup> and 3<sup>rd</sup> experimental groups, the residual Fe-binding ability of blood serum was statistically significantly reduced in the relation to the control group, respectively, by 19,9% and 24,2% (p<0,05). When drinking 20 µg Ge / kg b.w., a tendency to increase the aforementioned indicator by 8,3% was noted. In animals of all experimental groups, the percentage of the transferring blood saturation is higher than that in rats of the control group, respectively, by 51,3% (p<0,01) - 1<sup>st</sup> group; 17,8% - 2<sup>nd</sup> group; 120,6% - 3<sup>rd</sup> group (p<0,01). When using GeC the activity of aminotransferases (AST, and ALT) remained at the level of the analogues of the control group, and alkaline phosphatase increased in animals of the 1<sup>st</sup> group - by 18,8%; 2<sup>nd</sup> group - by 58,8% (p<0,05); 3<sup>rd</sup> group - by 32,5%. In female rats of the 1<sup>st</sup> and 3<sup>rd</sup> experimental groups, an increase in the level of urea and a decrease in the amount of creatinine in the blood were noted, but in the 2<sup>nd</sup> group - a tendency to increase the level of creatinine relatively to its level in animals of the control group was revealed. Substances content with an average molecular weight in the blood serum of female rats F1 of all experimental groups correspondent with the animals of the control group.