Predictors of adverse outcome of in vitro fertilization programs
Identification of factors determining both adverse and favorable outcome of IVF will increase the effectiveness of this method and optimize infertility treatment. Objective. The aim of the research is to determine the relationship between hormonal and cytokine status indicators with an adverse outcome of the IVF procedure in women with tubal-peritoneal infertility. Patients and methods. The study involved 120 women with tubal-peritoneal infertility who underwent a “short” protocol of stimulation of superovulation in the IVF program. Depending on the result, women were divided into two groups: group 1 - with clinically confirmed pregnancy (n = 40), group 2 - non-pregnant women (n = 80). The content of IL-1α, lL-1 ß, IL-1Ra and hormones in serum were determined by ELISA. Genotyping of polymorphic markers rs1800587 (C/T) of the IL-1α gene, rs16944 (С511Т) of the IL-1 ß gene and rs2234663 (2/4) of the IL-1Ra gene was performed. Results. Markers of adverse IVF outcome that had prognostic significance were: C/C genotype of the IL-1α gene (OR = 2 [0.9-4.6], p = 0.043113), serum IL-1a concentration <11.4 pg/mL (OR = 3, 2 [1.1-9.1], p = 0.025347), endometrial thickness on the 2<sup>nd</sup> day of the menstrual cycle was >5 mm (OR = 3.2 [1.1-9.1], p = 0.025347), the concentration of prolactin on the 3<sup>rd</sup> day of the menstrual cycle was >363.8 mU/mL (OR = 6.3 [1.8-22.2], p = 0.001745), genotype T/T of the IL-1 ß gene (OR = 15.8 [5.1-48.9], p < 0.001), the concentration of progesterone was <16 nmol/L (OR = 22.2 [2.3-170], p = 0.000057).