IMMOBILIZATION OF BIOMOLECULAR PROBES ON THE Si / SiO <sub>2</sub> SURFACE
Conditions for surface functionalization of SOI structures (silicon on insulator of nanowire transistors) as the sensor elements for a highly sensitive detection of ultra small amounts of biomolecules were optimized. Various variants of coupling of protein probes to silicon or glass surface using a silane with or without a subsequent bifunctional reagents activation: cyanuric chloride, di(N-succinimidyl) carbonate or 1,1'-thiocarbonyldiimidazole were considered. In order to provide a key factor – the shortening of the distance between the sensor element and the molecule-probe, which determines the effectiveness of target complex detection, we have carried out a systematic study of biomolecular probes immobilization on the solid phase supports. It was shown that immobilization of biomolecules using carbonyldiimidazole as the minimum length linker can detect immobilized molecule up to 0.03 ng.