The Compare of Whole Genome Amplification Methods for Their Applicationfor Trophoblast Cells Analysis by Comparative Genome Hybridization
The phenomenon of fetal cells’ presence in maternal blood opens interesting prospects for capabilities of noninvasive prenatal diagnosis (NIPD). Trophoblast cells circulate in blood of pregnant women and are a potential target for NIPD. One of the key points in the possibility of studying the genetic material of single cells is obtaining the quantity and quality of DNA sufficient for subsequent analysis. To compare three different methods of whole genome amplification (LA-PCR, DOP-PCR and MDA) a model experiment was performed. Analysis of genetic material isolated cells was performed by comparative genomic hybridization. As a result, an optimal method for the whole genome amplification of the genetic material of single cells in a model experiment was to determine the PCR with linker adapters (LA-PCR), which showed a uniform representation of the genome regions as compared to the other methods used.