BIOTECHNOLOGY OF CARBON NANOPATICLES IN THE INFECTIOUS DISEASES DIAGNOSIS
Objective. Development of technology for the construction of non-instrumental diagnostic test systems based on carbon nanoparticles with functionalized surface. Materials and methods. Functional properties of diagnostic preparations were evaluated by their ability to bind specifically to immobilized corresponding ligands on a solid phase. Solid-phase reactants (immunosorbents) were synthesized in accordance with the objectives to maximize detection sensitivity and physical substrate features. Stability was evaluated by changes immunosorbents analytical characteristics during storage in direct comparison with the same synthesized in situ. Results. A series of test kits, which are suitable for non-instrumental diagnostics of infectious diseases (HIV-1 and 2, measles, whooping cough, diphtheria, tetanus, pseudotuberculosis, syphilis, etc.), were designed. At the same time a unified arrangement of detection using the only diagnostic preparation: the conjugate G protein with carbon nanoparticles, was approved. Immunosorbents synthesis technology for each of the test systems with optimal analytical performance developed. Conclusion. Designed test systems demonstrate attractive analytical and operational characteristics, confirming the suitability of carbon nanoconjugates for use in systems of effective non-instrumental diagnostics of a wide range of infectious diseases.