РОЛЬ БАГАТОШАРОВИХ ВУГЛЕЦЕВИХ НАНОТРУБОК У ДЕСТАБІЛІЗАЦІЇ ГЕНОМУ КЛІТИН ЛІНІЇ 3Т3
The Objective to study the cytogenetic changes in the cells of mouse embryonic 3T3 fibroblasts (clone 31), exposed to multiwall carbon nanotubes. Materials and methods. 3T3 cells were treated with multiwall carbon nanotubes in two doses (100 and 400 mkg/ml) during 24 and 48 hours. Cytogenetic study involved an analysis of the mitotic regime and pathology of mitosis, fraction of cells with premature chromosome condensation, micronuclei, and nuclear protrusions. Metaphase plates were analyzed for modal class of chromosomes, polyploidy, the number of cells with chromosome aberrations, double minichromosomes and multiaberrant cells. Results. Multiwall carbon nanotubes have a pronounced genotoxic effect on mouse embryonic 3T3 fibroblasts, which was accompanied by formation of micronuclei and various abnormalities of nuclear shape. High doses of multiwall carbon nanotubes caused inhibition of the proliferative activity on phase G2/M of cell cycle and accumulation of chromosome aberrations and multiaberrant cells. Multiwall carbon nanotubes caused the dose-dependent delay of cell division in prophase and metaphase during 48 hours. Registered cytogenetic changes indicate the formation of genomic disorders and genomic instability in the cells exposed to carbon nanoparticles.