STUDIES OF PHYSIOLOGICAL CHANGES IN THE TISSUES OF THE PANCREAS
Light microscopy was used to examine histologically the pancreas of control rats that were subjected to acute experimental stress and those that were injected with melanin or placebo before modeling acute stress. The pancreas was fixed in a 10% solution of neutral formalin. After fixation, the material was washed through chloroform and chloroform-paraffin mixture and poured into paraffin blocks. After that, serial sections with a thickness of 5-6 microns were prepared. Review preparations stained with hematoxylin-eosin were used for general assessment of the condition of the studied tissues. Microphotography of the selected areas was performed using a microscope with a biorex 3 digital microphotometer. We analyzed the results obtained during the experimental part of the work using the methods of variation statistics. To check the distribution for normality, we used the calculation of the Shapiro-Wilk criterion. If the data corresponded to the normal distribution, the reliability of their difference when comparing the arithmetic mean values was determined using the Student's t-test for independent samples. Those that correspond to p<0.05 were considered reliable. When the data were not subject to normal distribution, statistical processing was performed using a nonparametric method - the Mann-Whitney test.