ESTIMATION OF CYTOKIN-LIKE ACTIVITY OF STAPHYLOCOCCUS AUREUS DEPENDING ON THE AVAILABILITY OF GENETIC DETERMINANT OF STYPHYLOCOCCAL PROTEIN A
In this work, the production of cytokine-like substances was studied in 5 strains of Staphylococcus aureus, with and without genetic determinants of staphylococcal protein A (spa gene). The spa gene in S. aureus was determined by the polymerase chain reaction (PCR) method. The cytokine-like activity of the supernatants of S. aureus daily broth cultures was determined using Multiplex (Luminex, United States) immunofluorescence test systems (G-CSF, GM-CSF, INF-γ, IL-1Ra, IL-1β, IL-2, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-17a, TGFα, MCP1, MIP1b, TNF-α) on a MAGPIX-100 instrument (USA). It was established that, regardless of the presence or absence of genetic markers of staphylococcal protein A, in the supernatants of the studied strains of S. aureus, cytokine-like substances were detected. Thus, the cytokine-like activity of S. aureus does not depend on the presence in bacteria of the spa gene, which is responsible for the production of staphylococcal protein A.