DEPENDENCE THE SPECTRAL PROPERTIES OF NEW PHOTOACTIVATED FLUORESCENT DYE FROM THE PARAMETERS OF THE MEDIUM
We presented in this article The investigations of dependence the spectral properties of the novel photoactivated fluorescent dye (PFD-813) from the parameters of the medium (viscosity and pH), and also the investigations of fluorescence concentration quenching. We presented that a power-law dependence of intensity from viscosity, corresponding to the Förster–Hoffmann equation; For example in solutions of glycerol-water with increasing viscosity by 1000 times fluorescence intensity increasing by 2.5 times. pH also influences on spectral properties of the dye, especially on its fluorescence intensity, which is by 1,5-2,5 times larger in cationic form, than in zwitterionic. The concentration quenching was observed already at concentrations from 6,310-5 М (30 µg/ml), this makes it possible to assess the optimal concentration values for staining of biological objects by the investigated dye. Using a dye concentration of 5 µg/ml (1,0610-5 М) microphotograph of the A 431 cell monolayer was obtained, in which the fluorescence intensity distinguished between the visually brightest and visually darker intracellular structures on the average from 5 to 10 times. Unequal brightness of different subcellular organelles can be explained not only by different selective staining of different subcellular organelles by the dye, but also by heterogeneity parameters of the intracellular environment.