INCREASING THE IMMUNOGENICITY OF THE INACTIVATED INFLUENZA VIRUS A/CALIFORNIA/7/09 (H1N1) USING AS ADJUVANT FUCOIDAN FROM BROWN ALGA FUCUS EVANESCENS
Objective: the aim of the research was to study the effect of sulfated polysaccharide from brown alga Fucus evanescens on the immune response to the inactivated influenza virus A/California/7/09 H1N1pdm. Materials and methods: BALB/c mice were immunized twice with inactivated pandemic influenza virus A/California/7/09 H1N1pdm09 with fucoidan. The level of hemagglutinating antibodies to the virus in the RTGA in the blood serum was determined 2 weeks after a single and double immunization. Results: in primary and secondary immunization fucoidan exerted an adjuvant effect, stimulating the formation of antibodies to influenza virus A, and in response to the introduction of a low dose of the virus (5 μg/mouse), antibody titers did not differ from the administration of high dose (15 μg/mouse), which it is important for choose a vaccination dose. The effect of fucoidan on the formation of antibodies to influenza virus A was expressed to a greater extent compared with the traditional licensed adjuvant aluminum hydroxide. Conclusion: the addition of fucoidan to the inactivated influenza virus А, an experimental analogue of the vaccine strain, significantly increases its immunogenicity, which indicates the promise of using fucoidan as an adjuvant in influenza vaccines.