Biosynthesis of hexaand pentameric chitooligosaccharides using n-acetylglucoseaminyl transferase from rhizobial bacteria
Chitooligosaccharides find wide application that determines considerable interest in their use. Enzymatic synthesis of hexa-N-acetylchitohexaose and penta-N-acetylchitopentaose using N-acetyl-glucoseaminyl transferase enzyme possessing unique features from rhizobial bacteria Rhizobium sp. GRH2 and M. loti has been performed in E. coli cells. Cultivation of bacteria E. coli expressing the appropriate recombinant enzyme resulted in synthesis of significant amounts of desired chitooligosaccharides (milligrams per liter). Analysis of synthesized chitooligosacchairdes by methods of high performance liquid chromatography and mass-spectrometry confirmed the conformity of the synthesized compounds to standards.