INFLUENCE OF THE "LIGFOLUM" NATURAL ADAPTOGEN ON CHICKEN EMBRYO BLOOD MORPHOLOGICAL COMPOSITION
Previously, we established a positive stimulating effect of the Ligfol solution in the concentration of 1.5% on the growth of chicken embryos and the development of their immunocompetent organs. The purpose of this work was to study the effect of the «Ligfol» natural adaptogen on the chicken embryo blood morphological composition. The work was carried out on hen embryos of the «Hysex Brown» egg cross. In the experimental group, deep processing («vacuum suction method») of eggs was carried out according to the method of F.I. Suleimanov. As an adaptogen of natural origin, a solution of «Ligfol» in the concentration of 1.5% was used. Eggs were laid for 20 minutes in a solution with a temperature of 16 °C. In the control group, the eggs were not subjected to the pre-incubation treatment. Incubation of eggs both in the control and in the experimental group was carried out from the first day in the I-32-1 automated incubator at a stable mode with an air temperature of 37.6±0.1 ° C and a relative humidity of 55.0%. Blood samples were taken on the 13th, 15th, 18th and 20th incubation days. The counting of erythrocytes and leukocytes was carried out according to the classical method using the Goryaev camera. The blood hemoglobin content was assessed using the Drabkin hemiglobin cyanide method. For the calculation of leukocyte indices, blood smears were prepared with further staining according to Romanovsky-Gimza. The results of the research showed that pretreatment of incubation eggs with Ligfol solution had a stimulating effect on the morphological composition of blood on certain days of embryogenesis: an increase in the total number of erythrocytes in the chicken embryo blood on the 15th, 18th day, hemoglobin - on the 20th day, leukocytes - on the 18th day, pseudoeosinophils - on the 18th day, monocytes - on the 15th day. Additional stimulation of eggs with natural adaptogens can be used in the industrial practice of poultry egg incubation as well as in the study of the synergistic effect of pre-incubation eggs treatment and variable incubation temperatures on the embryonic development of chickens.