EFFECT OF INSULIN, THE GLUTATHIONE SYSTEM AND SUPEROXIDE ANION RADICAL IN MODULATION OF LIPOLYSIS IN ADIPOCYTES OF RATS WITH EXPERIMENTAL DIABETES
It was found that spontaneous lipolysis increases in adipocytes of rats with alloxan diabetes; besides, isoproterenol- stimulated hydrolysis of triacylglycerols is inhibited against the background of oxidative stress and decrease redox-status of cells. A decrease in the ability of insulin to inhibit isoproterenol-stimulated lipolysis in adipocytes that were isolated from adipose tissue of rats with experimental diabetes was detected, which shows a disorder in regulation of lipolysis in adipocytes by the hormone in alloxan diabetes. Based on these findings, it was concluded that there is an influence of reactive oxygen species, superoxide anion radical, in particular, and redox-potential of the glutathione system on molecular mechanisms of change of lipolysis intensity in rat adipocytes in alloxan-induced oxidative stress. Activation of spontaneous lipolysis in conditions of oxidative stress may be one of the reasons for the high concentration of free fatty acids in blood plasma in experimental diabetes, and this may play a significant role in development of insulin resistance and appearance of complications of diabetes.