Immunologycal detection of hemodilution in bone marrow aspirates (based on M. Loken’s test)
The bone marrow is a mean subject of research in oncogematology. The results of accurately estimation and enumeration of small cells population (progenitor cells, cells of minimal residual disease, blasts) may be dependent on dilution of bone marrow with peripheral blood. Hemodilution is measured by flow cytometry using intensity of CD16b. Expression of antigen distinguishes mature neutrophils and maturating granulocytic cells. Detecting hemodilution reduces variability introduced in results and make a measurement of rare cell population more precious.