THE USE OF AUTOLOGOUS ERYTHROCYTES FOR TARGETED DELIVERY OF ASCORBIC ACID TO THE LIVER IN ORDER TO OPTIMIZE THE ANTIOXIDANT ACTIVITY OF HEPATOCYTES AFTER HEMORRHAGE
The aim of the research is to study the influence directed transport of ascorbic acid in the liver to its antioxidant activity after blood loss. Numerous studies have found that blood loss is accompanied by activation of lipid peroxidation in the liver. The direction and intensity of these processes depend on the state and degree of mobilization of antioxidant protection. The antioxidant capacity of the liver is not limited to hepatocytes. The liver synthesizes proteins of blood plasma. This antioxidant protein buffer system provides protection primarily at the level of red blood cells, preventing them from hemolysis. A promising direction in the correction of the functional status of the liver is to use direct transport of drugs in the organ. One of the most suitable carriers of medicines by virtue of security, ease of preparation and efficacy in the clinical practice are erythrocytes. Wall of hepatic sinusoids does not contains pericytes and presented at a large distance for only endothelium which has the ability to phagocytosis of erythrocytes. As a matter of optimizing the antioxidant activity of the liver we was chosen ascorbic acid, which is an donor of electrons and has the ability to inactivate various free radicals. The erythrocytic containers with ascorbic acid were produced according to the method of hypotonic lysis. Erythrocyte containers with ascorbic acid were administered intravenously in doses of 25, 50 and 100 mg/kg once after 10 minutes after blood loss. The antioxidant capacity was evaluated by the liver catalase activity in hepatocytes, albumin content in serum. It has been shown that the use of autologous blood cells for targeted delivery of ascorbic acid to the liver increases the pool of endogenous antioxidant protection.