Тransplantation of adipose tissue-derived mesenchymal stem cells in combined graft for peripheral nerve recoveryin rats - experimental study
Today the development of cell technologies, regeneration features of the damaged peripheral nerves, the experience of the use of stem cells on this pathology give us the opportunityto state that the surgical technique of peripheral nerve recovery, using a conduit and a cellular component is one of the most promising. During experimental study we carried out morphological evaluation of the recovery of peripheral nerve in laboratory animals, morphometric analysis of the total number of blood vessels and myelinated fibers in the proximal stump, the central part and the distal stump in all experimental groups. We used method of evoked muscle potentials - H-reflex and M-responses and analyzed the electrophysiological parameters of monosynaptic H-reflex and M-response in order todetermine the conductivity on the sciatic nerve and the intensity of demyelinating process. The study found that the transplantation of adipose tissue-derived mesenchymal stem cells (ADSC) and ADSC differentiated in Schwann cell-like cells is an effective technology and can be recommended for use in clinic.