ACTIVITY OF NUCLEOLAR ORGANIZERS IN HEPATOCYTES OF RATS WITH CCL4-INDUCED CIRRHOSIS AFTER TREATMENT WITH BIOACTIVE PREPARATIONS
The therapeutic efficiency of bioactive preparations of human fetal liver (extract, cell-rich fluid), chorionic gonadotropic hormone and «Maksar» (plant-extracted product of Maakia amurensis, kindly provided by S.A. Fedoreev, Pacific Institute of Bioorganical Chemistry, Vladivostok, Russia) in the treatment of model toxic liver cirrhosis is shown. Toxic liver cirrhosis was induced by 2-months i.p. administration of carbon tetrachloride (CCl4) to male rats. Treatment was performed within 14 days after last CCl4 injection. Evaluation of nucleolar organizer activity (AgNOR) showed that livers of intact rats and treated rats contained mostly type I hepatocytes (low proliferative activity), whereas untreated rats had mostly type II hepatocytes (high proliferative activity). On the contrary, amount of argentaffin granules per nucleolus was significantly higher in treated groups compared to control and CCl4-intoxicated rats. The data obtained indicate that all the preparation under consideration are capable of modulating the hepatocyte regeneration, and human fetal liver extract and cell-rich fluid have the most prominent effect.