Isolation of FVIII/VWF complex from donor plasma
Summary. Laboratory method for isolation and purification of factor VIII (FVIII) - von Willebrand's factor (vWF) complex is based on a combination of anion exchange chromatography with precipitation methods. The PEG-4000 precipitation conditions are optimized, due to which at least 65% main ballast protein (fibrinogen) is removed, and the mean output of FVIII at this stage reaches at least 70%. At the ion exchange chromatography stage the FVIII output is at least 98%. The main parameter determining the proportion of components in the resultant FVIII/vWF complex is the specific activity loading of the adsorbent. This method allows variations of vWF from 1.9 to 0.23 U/U FVIII. The results can serve the base for technology for creation of drugs for the treatment of patients with hemophilia A and von Willebrand's disease.