SYNTHESIS AND BIOLOGICAL BEHAVIOUR EVALUATION OF Tc(CO)3 + COMPLEXES WITH DITHIOCARBAMATE LIGANDS CONTAINING MORPHOLINE MOIETY
The purpose of this stadywas to synthesize and study complexes of 99mTc(CO)3 + (99mTc-TC) with dithiocarbamate ligands included morpholine moiety which have high affinity to melanoma. To study cell uptake and efflux of this complexes in melanoma B-16 and adenocarcinoma MCF-7 cell lines. To study of biological dehavior of this complexes in melanoma-bearing mice. In this study are used cells of melanoma В-16 and breast adenocarcinoma MCF-7 for <i>in vitro </i>experiments and СВАхС57Вl black mice with implanted melanoma B 16 for <i>in vivo </i>studies. The technetium complexes with various ligands were obtained in two steps.99mTc-TC-Cit and 99mTc were used as comparative radiopharmaceuticals. The cell uptake of the received complexes <i>in vitro </i>estimated in 30, 60, 90, 120 and 180 minutes. Viability of cells estimated by means of a staining by trypan blue.