Therapeutic Efficacy of Liposomal Quercetin with Different Routes of Administration in a Model of Moderate Blunt Trauma to the Eyeball
Introduction. The paper is focused on a topical issue of searching effective pharmacological agents with comprehensive effect enabling to influence simultaneously on several components of a posttraumatic process. The purpose of the present paper was to study the efficacy of liposomal quercetin with different routes of administration in a model of blunt trauma to the eyeball in rabbits. A special device with a force of 2-3 nm to an eye was used to simulate a moderate blunt trauma. A total score of three clinical signs, including ocular hyperemia, the state of the cornea, and the presence of hyphema, was used for comparative analysis. Lipoflavon was used as instillations in combination with subconjunctival (5 eyes) and subtenon (5 eyes) injections. 5 untreated rabbits (5 eyes) served as control. Results. The total response of the eye to blunt trauma was significantly decreased in the rabbits treated with Lipoflavon compared to untreated control. A combination of Lipoflavon instillations with subtenon injections decreased the total eye response to blunt trauma in a greater degree as compared to that with subconjunctival injections, equaling, respectively, 4.8±0.3 and 5.6±0.2 points vs. 7.0±0.4 of control at Day 3; and, respectively, 3.6±0.3 and 4.0±0.0 points vs. 5.4±0.2 of control at Day 7. The clinical signs reached normal values in both study groups within 15 days with 3.8±0.2 of control. Conclusions. Combined instillations and periocular injections of Lipoflavon for experimental blunt trauma to the eye had a positive effect on the course of posttraumatic processes in the rabbits’ eyes. Combined treatment can provide the conditions for the best concentration of the drug in the affected area. The ambivalence of liposomes supports their penetration through hydrophilic and lipophilic media in the eye, so targeted delivery of Lipoflavon in periocular drug administration routes is very promising.