Soluble membrane antigens CD95, CD25, CD54, CD38 in acute pancreatitis and their possible clinical relevance
Soluble forms of membrane antigens are important molecular factors of the immune system. Their role in the pathogenesis of the systemic inflammatory response, organ dysfunction (OD) in acute pancreatitis (AP) poorly understood. The studying of the serum levels of the soluble forms of membrane antigens CD95, CD25, CD54 and CD38 (sCD95, sCD25, sCD54, sCD38) is carried out in AP of various severity. Level of soluble antigens detected in serum of 37 patients with OP: 28 with necrotizing pancreatitis (NP) and 9 with interstitial pancreatitis (IP). A control group consisted of 25 healthy donors. The revised Atlanta criteria applied for AP classification. Immune-enzyme assay have been use to determine the level of sCD95, sCD25, sCD54, sCD38. It revealed that within 1-4 days of IP and NP without OD the serum level of sCD95 did not change. At NP with OD the sCD95 level increased. The formation of necrotic fluid collections accompanied by sCD95 increase on 5-18 days. Serum level of sCD25 within the first 4 days of the disease was lower in IP and NP without OD. At NP with OD it was significantly higher, than at IP. Similar results received at an assessment of sCD38 serum level. The high level of sCD54 found in NP cases with adult respiratory distress syndrome development.