Adaptive Plasticity in osmotic stress of biotech canola ( <i>Brassica napus </i>l.) possessing <i>cyp11A1 </i>or simultaneously <i>desC </i>and <i> epsps </i>transgenes
In order to study the osmotic stress tolerance of canola (Brassica napus L.) plants constitutively expressing cyp11A1 or simultaneously desC and epsps genes that encode bovine cytochrome P450 <sub>SCC</sub> or ∆9 desaturase from cyanobacterium Synechococcus vulcanus and bacterial enolpyruvat shikimat phosphate syntase, respectively, the influence of different osmotic pressures induced by mannitol addition on plant in vitro growth was estimated by evaluation of fresh weight (FW), total soluble protein content (TSP), and superoxide dismutase activity (SOD). Transgenic cyp11A1 lines formed higher FW and TSP (by 30% and 36%, respectively) as well as they had up to 1.65-fold higher SOD activity in comparison with initial plants under normal conditions. FW and SOD activity of cyp11A1 plants were higher and TSP was similar compared the control plants under osmotic stress. Biotech desC/epsps lines showed no differences compared with untransformed plants both in normal and stressful conditions. Adaptive plasticity to osmotic stress of cyp11A1canola was higher than ones of initial and desC/epsps plants. It may be due to increase in SOD activity