Immunohistochemical characteristics of expression of thyroid stimulating hormone and vascular endothelial growth factor
Purpoze of the study. Immunohistochemical analysis of TSH and VEGF expression in the diagnostic evaluation of various forms of goiter. Material and Metods. When studying the expression of TSH and VEGF, samples of waxed tissues of fixed 12% for malin were used. Incubations were performed with anti-VEGF165 antibodies and anti TSH clone antibodies (“BioGenex” ABŞ) at a broken ratio of 1:50. Conclusions. Determined, that adenomatous multinodular goiter unlike diffuse toxic goiter detected more higher coef ficient nuclear death - 1% and lower mitotic coefficient - 0,2%. Diffuse toxic goiter is characterized by a pronounced expansion of the capillary lumen. Results. Instead, with diffuse toxic goiter the diameter of the lumen of the capillaries, cytoplasma and core thyrocytes, number of follicles and thyrocytes is significantly higher compare to multinodular toxic goiter.