Comparison of the results of per oxidation (free radical) of liver lipids at the background of hexamethonium injection and 3-hour exposure to cold of animals with the results of the induce peroxidation of lipids of liver microsomes with hexamethonium in vitro
Method of the stimulation of peripheral muscarine sensitive cholinoreactive structures of metabototrophine G-proteins with endogenous acetylcholine presumes a preliminary injection of peripheral, non-selective nicotine - sensitive cholinolytic to experimental animals. Before the application of the method the influence of peripheral, non-selective nicotine - sensitive cholinolytic hexa-methonium on the content of the diene conjugates (DC), hydroperoxides (HP) of common lipids (AL) of the liver and liver malondialdehyde (MDA) of liver homogenate after the 3-hour exposure to cold in animals was determined. The results of hepatic lipid oxidation obtained in vivo at hexamethonium injection to animals were compared to the results of oxidation of lipids of liver microsomes made in vitro with hexamethonium. The induction of lipid peroxidation in vitro was carried out with enzymatic (NADP • H-dependent) mechanisms. Comparing the results of lipid oxidation in vivo and in vitro the hypothesis on the influence of block of peripheral, nonselective nicotine - sensitive cholinolytic structures ligand of conductive ion canals of plasmatic membrane of hepatocytes with hexamethonium on the lipids peroxidation (free radical) could be made.