GENTAMICIN AND NEOMYCIN DETERMINATION IN FOOD BY POLARIZED FLUORESCENCE IMMUNOASSAY
Developed the determination techniques of gentamicin and neomycin in food by fluorescence polarization immunoassay. Synthesized tracers, calculated tracers constants (KT) and polyclonal antibodies affinity constants (KAf), selected the best immunoreagents pair for antibiotics determination. Calculated cross-response coefficients (PR %), which values indicate the possibility of selective determination of gentamicin and neomycin with the presence of related structure compounds. The calibration curve was linear in the 40-5100 ng/ml range for gentamicin and 70-2800 ng/mL range for neomycin, detection limits for gentamicin and neomycin are 9 and 61 ng/ml, respectively. Studied extraction systems based on acetonitrile, hexane, dichloroethane, acetone, methanol and a phosphate buffer solution for separation of neomycin and gentamycin from the meat and eggs. The developed method was tested for the analysis of chicken meat, eggs, milk.