Determination оf Amikacin by Rayleigh Scattering Method Based on the Covalent Bonding of The Analyte with a Water Soluble Polymer
A method for the determination of aminoglycoside antibiotics based on the covalent bonding of the analyte with a water-soluble polymer by Rayleigh scattering method (λ <sub>ex</sub> = λ <sub>em</sub>) is proposed. Determination of amikacin (as model analyte) is carried out by the carbodiimide crosslinking technique that forms amide bonds between the co-polymer of 4-styrenesulfonic acid and maleic acid and primary amine groups of amikacin. Conditions of the reaction have been selected to obtain maximum scattering intensity of the product: reaction time 15 min, pH 7.0, ionic strength 0.02, measuring of scattering intensity at 362 nm. The determination of amikacin is not influenced by (mol/mol ratio): Al(III), Co(II), Cu(II), Fe(III), Ni(II), Zn(II) - 300, Cl <sup>-</sup> and NO <sub>3</sub> <sup>-</sup>- 250, Ca(II) and Mg(II) - 50, HCO <sub>3</sub> <sup>-</sup> - 20; nitrogen compounds affect at 20 - 50-fold, protein - 2.5-fold ratio. The calibration plot was constructed over the range of 0.2- 35 mg/L ( r = 0.993), LOD (3 s -criterium) is 0.08 mg/L ( n = 10, P = 0.95). The analyses on the drug powder for injection and gel have been performed.