THE ROLE OF GLUTAREDOXIN AND GLUTATHIONE IN PROLIFERATION OF BREAST CANCER CELLS UNDER THE EFFECT OF 1,4-DITHIOERYTHRIOL, A THIOL GROUP PROTECTOR
The objective. To determine the role of glutaredoxin and glutathione in regulating proliferation of MCF-7 breast cancer cells under the effect of 1,4-dithioerythriol, an SH group protector. Materials and methods. The study was conducted in the culture of MCF-7 human breast cancer cells. We modulated the redox status of MCF-7 cells with 1,4-dithioerythriol, a protecting agent for SH groups. We estimated the reduced/oxidized glutathione ratio, the concentration of protein SH groups, glutaredoxin, cyclin E, cyclin-dependent kinase 2, and cyclin-dependent kinase 4. We also evaluated the activity of glutathione reductase and glutathione peroxidase in intact tumor cells and in the presence of 1,4-dithioerythriol. Results and discussion. We found out that 1,4-dithioerythriol reduces the activity of MCF-7 cells. We showed that glutathione and glutaredoxin play an essential role in redox-dependent modulation of the functioning of proliferation-regulating intracellular proteins. This research area is promising for developing personalized approaches to diagnostics and therapy of breast cancer.