PATHOLOGY OF GLIOBLASTOMA DEPENDING ON THE PRESENCE IDH-1 GENE MUTATION
Introduction. Glioblastoma is the most common high-grade brain tumor with predominantly astrocytic differentiation. Glioblastoma with IDH-1 gene mutation, that occurs during the early stages of differentiation of tumor cells, have a more favorable overall survival.Objective. Identification of the pathological differences between glioblastomas with IDH-1 gene mutation and without it.Materials and methods. 37 cases of glioblastoma were subjected to pathological examination. Immunohistochemical study was performed with the antibody Anti-Human IDH-1 (Dianova clone: ​​H09, 1:20 dilution).Results. Depending on the cellular structure, pathological examination of glioblastomas allowed to differentiate the following types of glioblastoma: glioblastoma multiforme - 23 cases, giant cell glioblastoma - 7 cases, gliosarcoma - 7 cases.There were focal necrosis with pronounced pseudopalisades in the IDH-1 positive glioblastomas, mitotic figures ranged from 9 to 21 in 10 fields of view, the number of proliferating vessels ranged from 5 to 10 in 10 fields of view. In the IDH-1 negative glioblastomas necrosis were extensive and occupied more than ½ of the field of view, often without pseudopalisades, number of mitosis ranged from 20 to 28 in 10 fields of view.Conclusion. The study revealed the existence of differences in the pathological picture of glioblastomas with mutations of the gene IDH-1, and without it, specifically in necrosis, mitotic activity figures and microvascular proliferation.