OXIDATIVE MODIFICATION OF PROTEINS RATS SPLEEN WITH EXPERIMENTAL NITRIC OXIDE SYNTHESIS DIFFERENT EXPRESSIONS
The influence of L-NAME at the dose of 25 and 200 mg/kg on the oxidizing modification of the proteins of the rat’s spleen was studied. It is stated that in the conditions of the simulation of the scarcity of the synthesis of the oxide of nitrogen(II) a quantity of carbonyl derived increases and the content of bityrozin and oxidized tryptophan is reduced. After using N-nitro-L-argininmethyl ester at the dose of 25 mg/kg the level of protein carbonyl derivatives of rat’s spleen increases in comparison with the control group. It is important, that the amount of aldehyde- and ketone- dinitrophenyhydrazone significantly increases, which indicates about the damage of basic amino acids remains. The redoubling of oxidizing stress occurs under the action of L-NAME at the dose of 200 mg/kg because the second markers -ketone- dinitrophenyhydrazone are formed and accumulated.