Partial hepatectomy against the background of superinvasive opisthorchiasis does not provoke carcinogenesis
Research goal: identification of the risk of oncogenesis in the liver under the influence of 2 promoter factors - partial hepatectomy (PHE) performed against the background of superinvasive opisthorchiasis (SO) in Syrian hamsters. Material and methods. SO was modeled by infecting animals (n = 86) with 50 metacercariae of O. felineus. Superinvasions (50 metacercariae) were repeated 6 and 16 days after primary infection. PHE - removal of the middle lobe of the liver - 17.3-17.7% of the organ weight was performed according to the method of G. M. Higgins, K. M. Andersson 16 days after the last superinvasion. The quantitative assessment of the regenerative process after PHE was determined by the coefficients of regeneration completeness (%) by the formula Кпрп = (М1 - М2) / М3 × 100. The histological specimens were stained with hematoxylin and eosin and by the methods of Van Gieson, Slinchenko, Samsonov, Kupriyanov. IHC-reactions were carried out with antibodies to Ki-67, CD31, CD34, CD117, Oct 4, and α-fetoprotein. Electron microscopic analysis was performed on a “JEM - 100CX” microscope (JEOL, Ltd., Japan). The quantitative characteristics were processed using the statistical software Microsoft Excel (2019) and the Statistica package (version 12.6). Results. Analysis of the obtained data indicates that by the 16th day after PHE, 3 zones have formed in the liver: zone A - the stump, zone B - is adjacent to the wound zone A, zone C - is adjacent to zone B. By the 16th day after PHE, expressed proliferation processes of the CD31, CD34, Oct 4, CD117-positive cells and differentiation into endothelial cells, cholangiocytes, hepatocytes with the formation of vessels are noted. At a later date of the experiment, hepatocyte fields with the presence of α-fetoprotein in the cytoplasm, cholangiocellular tubules were formed; at a later date (23 days), ducts lined with a cylindrical epithelium of normal structure were identified. The epithelial lining of the preexisting ducts is multi-row by the end of the experiment; no tumors were found in the liver in the experimental group. An increase in liver mass was noted in the control group (SO) by 8.2%, in the experimental group - 24.7%. Conclusion. The liver with partial hepatectomy against the background of superinvasive opisthorchiasis is an organ with permanent proliferation of CCD and HCD cells, hyperregeneration and a significant increase in mass. In the presence of 2 promoters without initiators, the risk of carcinogenesis does not increase.