Comparison of distribution of erythropoietin medications isoforms with two-dimensional gel-electrophoresis
Purpose. Comparison of physicochemical properties of different preparations of recombinant human erythropoietin. Material and methods. Preparations of recombinant human erythropoietin - Eprex ® and Eralfon® - were compared with each other using two-dimensional gel electrophoresis and polyacrylamide gel electrophoresis over sodium dodecyl sulfate (SDS-PAGE electrophoresis) with Western blot. Results. The degree of glycosylation of erythropoietin in the drug Eprex ® is exceed the same in the drug Eralfon ®. More basic isoforms of erythropoietin in the preparation Eralfon ® may indicate a short half-life. Eralfon ® contains a significantly greater number of isoforms of erythropoietin as compared with the drug Eprex ®. Conclusion. Physico-chemical properties of recombinant human erythropoietin are different, that can lead to the change of their efficacy and tolerability.