Development of new lentiviral transfer vector for ectopic expression of single-chain insulin-coding gene in human mesenchimal stem cells
An actual problem nowadays is increasing frequency of diabetes mellitus. The technology of stem cells as well as genetic engineering methods being combined open new perspectives for therapy of this disease. We have developed new lentiviral transfer vector pHR-hPPI carrying human single-chain insulin gene under control of constitutive promoter. The vector was used for transduction of human bone marrow-derived mesenchymal stem cells. More than 90 % of transduced cells were stably expressing eGFP reporter protein during 24 days. The quantitative PCR analysis showed that insulin gene transcripts number was 200-300 times more in transduced cells comparing to nontransduced cells, but still 10 times less comparing to pancreatic islet cells.