REDOX STATUS OF ASCITIC FLUID AT DIFFERENT STAGES OF EXPERIMENTAL OVARIAN CANCER
In Russia, ovarian cancer (OC) is the first cause of mortality from vaginal cancer. Ascitic form of OC is considered to be the most malignant. The ascites composition is determined by the neoplasm biological properties in its development. The aim of the study was to evaluate the redox status ofascitic fluid at various stages of experimental OC. Materials and Methods. The work was performed on white outbred rats, 180-200 g, with a transplanted ascitic ovarian tumor. Ascites was taken from animals in the stationary and terminal stages of tumor growth. After removal of tumor cells, lipid peroxidation parameters were biochemically determined in it: diene conjugates, ketodienes, Schiff bases, malondialdehyde, levels of protein oxidative modification (346 nm - neutral aldehyde groups; 370 nm - neutral ketone groups; 430 nm - basic aldehyde groups; 530 nm - basic ketones) and antioxidant defense components - catalase and glutathione-S-transferase activity. The results were evaluated by means of Mann-Whitney U test (Stata 6.0). Results. As a result of the research conducted, the authors determined a significant reduction in the level of protein oxidative modification, a decrease in the catalase activity and a decrease in the malondialdehyde level at the terminal stage of tumor growth as compared with the stationary stage indices. Conclusion The redox status of ascitic fluid in experimental OC is characterized by a decrease in the oxidative modification of proteins, a decrease in the intensity of lipid peroxidation and a constant catalase and glutathione-S-transferase activity in the dynamics of neoplasm progression.