CHARACTERISTICS OF LOCAL CELLULAR IMMUNITY IN COLON CANCER DEPENDING ON TUMOR LOCATION
The role of various components of the immune system in the development and clinical course of malignant tumors is an urgent issue in modern oncology. Colon tumors are infiltrated by various immune and inflammatory cells, and a number of studies show that the number of T-lymphocytes. The purpose of the study: to reveal characteristics of the local populations and subpopulations of immunocompetent cells in colon cancer with various tumor locations. The study included 50 patients with colon cancer. Cell suspensions were prepared from the tumor fragments, peritumoral tissues and resection line tissues. Сell suspensions were processed with a panel of antibodies to identify the main populations and subpopulations of leukocytes and lymphocytes using the BD FACSCanto flow cytometer. A decrease in lymphocytic infiltration was determined in tissues of left-sided tumors, compared to right-sided ones, by 47%. Left-sided tumors were characterized with 28% higher tumor infiltration with NK cells, compared with right-sided tumors. The perifocal tissues of left-sided colon tumors showed a decrease in the relative numbers of lymphocytes, NK, NKT and CD19+ by 59%, 26%, 34% and 30%, respectively, compared with right-sided tumors. A significant increase in the numbers of CD8+- and DP cells was revealed - by 107% and 425%, respectively, in left-sided colon cancer, with the absence of changes in CD3+-, CD4+-cells. The remote area in left-sided tumors showed an increase in lymphocytic infiltration, the percentage of CD8+ cells and DP by 25%, 34% and 50%, respectively, compared to right-sided tumor location.