Detection of DNA in Human Blastocyst Cavity Aspirate by Multiplex PCR
Preimplantation genetic diagnosis is a modern method of detection of chromosomal and genetic abnormalities in human embryo before its transfer to the uterus. The genetic material is obtained by the embryo biopsy. In this study, an attempt was made to evaluate the effectiveness of the method of noninvasive biopsy – aspiration of the contents of blastocoel (blastocenesis) of the human embryos. In this research, a biopsy was done in human embryos of 6–7 days of development of low morphological characteristics. DNA obtained from aspirate, as well as from blastocyst, were analyzed by RT-PCR after the whole genome amplification. In total, 24 blastocysts and samples of aspirate obtained from them were analyzed, 6 in 7 (29%) samples, an assayable DNA was obtained, which in 5 (71%) cases was identical to blastocyst DNA. Thus, with a non-invasive biopsy of the human embryo in the blastocyst stage, it is possible to obtain DNA suitable for analysis by molecular genetic methods. The features and advantages of using the multiplex QF-PCR method in combination with full-genomic amplification for DNA research of the blastocoel fluid obtained during aspiration are discussed here. The prospects of the noninvasive biopsy method for use as a diagnostic procedure in the routine practice of infertility treatment and prevention of chromosomal and genetic abnormalities in newborns are considered in this work.