ЯКІСНІ ТА ВІДТВОРНІ ПОКАЗНИКИ СПЕРМИ БАРАНІВ-ПЛІДНИКІВ ЗА ВИКОРИСТАННЯ У СКЛАДІ СЕРЕДОВИЩА ДЛЯ ЗАМОРОЖУВАННЯ ВІДНОВЛЕНОГО ГЛУТАТІОНУ ТА БСА
The research findings on the determination of the optimal concentration of reduced glutathione and bovine serum albumin in the lactose-yolk-tris-citrate-glycerine diluent with ram-sires sperm cryopreservation are revealed. It is established that the addition of 5 µМ of reduced glutathione and 15 mg/ml of bovine serum albumin into the lactose-yolk-tris-citrate-glycerine diluent contributed to the increase in frozen-thawed sperm activity by 11,0 %, its resistance to cryopreservation by 6,0 %, intact acrosome by 9,5 %, sperm surviving rate by 23,9 %, absolute survival indicator by 21,8 %, sperm kinetic indicators: VCL - by 14,3 %, VAP - by 12,5 %, VSL - by 15,4 %, LIN - by 0,4 %, STR - by 2,2%, which indicates a positive effect of the added bioactive substances into the diluent on the qualitative characteristics of frozen-thawed sperm. At the same time observed the reduction in the number of TBA-active products in seminal plasma after equilibration by 22,2 % (p<0,01), after sperm thawing - 27,4 % (p<0,01) respectively in comparison with the control. Assessment of the reproductive indicators of ewes showed that the proposed improved diluent increases the biological value of sperm and their fertilizing capacity. In particular, the fertility of ewes inseminated with frozen-thawed sperm frozen in the improved diluent was higher by 12,0 % in comparison with the animals, inseminated with the frozen sperm using the control diluent