DEVELOPMENT OF REGULATIONS FOR PRODUCTION OF POSITIVE DNA CONTROL FOR THE PCR DIAGNOSIS OF ASF
The paper is dedicated to the development of recombinant positive control for the detection of DNA of African swine fever virus (ASF), based on the method of TA-cloning into plasmid vector pTZ57R / T. VP73-gene in the region of 278 bp, was amplified using PCR, and cloned within the recombinant vector into cells of E. coli strain HB10B. The ability to produce three clones of recombinant DNA that can be used as a positive control for PCR detection of ASFV was proved. The research results will be applied in the system of monitoring the disease in the study of clinical material by PCR.