DIAGNOSTIC IMPLICATION OF MOLECULAR-GENETIC TECHNIQUES IN ACUTE INTESTINAL INFECTIONS
Acute intestinal infections have remained a pressing issue to be solved by the public health service. In order to enhance the diagnostic efficacy and etiology interpretation potentials, species-specific primers for polymerase chain reaction (PCR) were selected and employed in the course of clinical material examination of most clinically significant representatives: <i>Enterobacteriaceae (Citrobacter spp</i>., <i>Hafnia spp.</i>, <i>Klebsiella spp</i>., <i>Proteus spp</i>. and <i>Escherichiacoli). </i>Refinements of DNA isolation and PCR techniques were made, which allowed us to recommend the selected pairs for diagnostic systems development.