Leukocytes respiratory metabolism and it’s evaluation for the development of a local polymer dosage form for treatment of periodontal infection
Purpose. Evaluation the metabolic activity of neutrophilic granulocytes and the effect of polymer-based film containing anti-inflammatory and antibacterial components on neutrophil respiratory metabolism. The research is conducted using luminol-dependent chemiluminescence stimulated by opsonized zymosan. Materials and methods. Two dental adhesive films have been analyzed in this study, “Diplen-Denta Ch” and “Diplen-Denta KPCh”. “Diplen-Denta Ch” with chlorhexidine is drug approved by Russian Ministry of Health and is currently used in Значение оценки респираторного метаболизма лейкоцитов при разработке местной лекарственной формы для лечения пародонтальной инфекции periodontology. “Diplen-Denta KPCh” is an experimental drug, which contains chlorhexidine, ketoprofen, and tocopherol acetate. The effect of both films on activity of neutrophilic granulocytes was assessed by luminol-dependent chemiluminescence, stimulated by opsonized zymosan. To conduct the assessment, peripheral blood of 26 healthy donors of age 20 to 25 years was studied. Results. Both investigated films had an effect on neutrophil granulocytes depending on cells initial functional state. In persons with low activation index (<3,054), “Diplen-Denta KPCh” caused a greater increase in spontaneous and stimulated chemiluminescence, as well as the activation index. “Diplen-Denta Ch” had only a modest increase in the activation index. In the group with high values of I(O) (>4,34), both drugs significantly decreased the activation index, while the relative magnitude of the I/I (O) index change in group 2 was significantly smaller than in group 1. Conclusions. Both investigated films have an influence on neutrophil granulocytes depending on initial functional state of the cells. The difference in effect of “Diplen-Denta KPCh” and “Diplen-Denta Ch” on neutrophils metabolic activity has been found to be related with the presence of ketoprofen