THE INFLAMMATORY RESPONSE IN EPILEPTIC FOCI IN PHARMACO-RESISTANT EPILEPSY
Search for the cause epilepsy of the occurrence and the development of new methods and means for relieving of seizures with prolonger, and ideally, lifelong remission are of ufmost important for neurologists today. Modern research is devoted primarily to the study of humoral immunity i9n pharmaco-resistant epilepsy. The aim our scientific study is to detect reactions of cellular immunity in the fragments of the temporal lobe in epileptic foci removed during the surgical treatment of pharmaco-resistant epilepsy in 11 patients with epilepsy from 2 to 15 years. The study was carried out with the help of immunohistochemical sample with antibodies to CD45 (total leukocyte antigen), CD3 (T-lymphocytes), CD8 (T-killer), CD20 (B-lymphocytes), CD68 (macrophages) (DAKO, Denmark).As a result our work, we discovered that, positive expression CD45 was found in all (100%) cases. In addition, using CD45 verified microglial cells. The comparison group served fragments of the temporal lobe of the brain from 5 patients who died without the diagnosis of epilepsy. In the comparison group cellular reaction was revealed in 2 from 5 cases, T-lymphocytes giving 100%. Microglia was not detected. The appearance of cells of immune system indicates damage of the integrity of blood-brain barrier and inflammatory response in epileptic foci. Thus, the appearance of cellular immunity reactions is essential and affects the course of epilepsy and its progress. Further study of the reaction of cellular immunity can become a source of solutions of many questions about the causes and methods of treating this disease.