IMMUNOCHEMICAL ANALYSIS EPIDIDYMIS AND TESTIICULAR PROTEOGLYCANS OF THE RATS
The purpose of the study is to get antibodies to proteoglycans of epididymis and testes in rats and then to conduct the analysis of diffusion and dynamics of the proteoglycans level. It has been shown that in order to get antibodies to proteoglycans in testes and epididymis in rats you should take hens to produce the antibodies. The analysis of the results of immunochemical study of proteoglycans in epididymis and testes in rats has been presented after the enzymatic moving away the carbohydrate component was realized by means of immobilized samples of chondroitinase AC and heparinase III. Two antigens of proteoglycan base of epididymis and testes have been identified in rats. One of the proteins was purified by combination of ammonium sulfate precipitation and gel-filtration of affine chromatography; and the protein is characterized as alpha-2 globulin with the molecular weight of 245-260 KD.