Development of methodology for production of a soluble form of recombinant human N-acetyltransferase-2
The work presents the development of a methodology and optimization of recombinant N-acetyltransferase-2 of human (hNAT2), found the optimal parameters of use of the enzyme in a soluble form and in sufficient quantity for the in vitro bioanalytical experiments. The paper presents data on the design of genetic constructs for efficient expression of recombinant enzyme human N-acetyltransferase 2, by the method of SDS-PAGE electrophoresis confirmed the molecular weight of the protein (34-35 kDa), indicated quantitative content of enzyme (3.14 mg/ml).