REACTION OF THE CELLULAR ELEMENTS OF THE BLOOD TO THE CONTACT WITH THE GRANULATED HYPERCROSS-LINKED POLYSTYRENE AND SILICA
The purpose of the study was the testing of granular polymer materials (hypercross-linked polystyrene MN-202 (HPS) and two silica - coarse-grained silica gel large-pore 2 (CSL-2) and silochrome S-120 possessing sorption properties as activating hemocontact preparations. Materials and methods. The hemocontact interaction was carried out under bench conditions using donor blood in a rotary mode. Blood samples were taken before the beginning of the experiment and after 5, 20, 40 and 60 minutes. Changes in cell and subcellular blood populations were assessed using the SySmex XT 1800i hematology analyzer (26 parameters), which made it possible to indirectly estimate the activation of blood cells. 45 experiments were conducted on 15 for each of the hemocontact preparations. Results. Erythrocytes did not react to contact interaction with sorbents. The degree of expression of activation properties of sorbents for platelets falls in the series: Silochrom S-120> HPS > CSL-2. For leukocytes, this indicator increases from CSL-2 <SilochromS-120 < HPS. CSL-2 has practically no adhesive properties for leukocytes, i.e. it doesn't activate leukocytes. The shift of the leukocyte formula (granulocytes / mononuclears) is most pronounced on the HPS granules (1,40→0,76), a bit less on Silochrome S-120 (1,41→ 0,97) and very weakly on CSL-2 (1,38→1,26). Conclusion. Approved granular polymer materials can be used as hemocontact preparations for the activation of blood cells (platelets, leukocytes) and changes in the spectrum (qualitatively and quantitatively) of bioactive substances in the blood when carrying out low-volume hemoperfusion