Standardization of the new composition of timogar
Procedures were developed to spectrophotometrically quantify novocaine hydrochloride and isoleucyl-tryptophan dipeptide in their simultaneous presence in a solution. Novocaine hydrochloride was assayed by direct spectrophotometry of the drug after its 500-fold dilution at a wavelength of 290 nm versus its standard solution sample. Isoleucyl-tryptophan dipeptide was determined after novocaine hydrochloride was isolated from the alkaline solution of the drug, by using ethyl acetate extraction. The parameters and methods for standardization of the new composition of the immunomodulator Timogar (timogar-nov) were determined by the following indicators: description, identity, clarity, color, and pH of solution, particulate matter, the content of novocaine hydrochloride and isoleucyl-tryptophan dipeptide, sterility, pyrogenicity, and toxicity. The identity of an agent was determined by ultraviolet spectrophotometry and thin layer chromatography (for dipeptide) and by qualitative tests for chloride and primary aromatic amines, those using potassium permanganate, sodium hydrochloride (for novocaine hydrochloride).