NEW APPROACHES TO MOLECULAR IMAGING OF THE BRAIN: 3D CYCLIC IMMUNOHISTOCHEMISTRY AND OPTICAL CLEARING
The review is devoted to the modern techniques required for 3D functional mapping of neuronal networks with cellular resolution. Different methods for cyclic immunohistochemistry, approaches to 3D immunohistochemical detection on thick nervous tissue samples, principles of optical clearing for signal visualization deep inside the specimen are described here in details. Main advantages and disadvantages of each method are analyzed. Perspectives for development of molecular brain mapping are proposed to detect neuronal substrates of cognitive functions in health and disease.