Protein profiling of candidate strains of bacterial composition
Organic farming and the application of organic and biological fertilizers and protective equipment is not only a promising, but also a safe way to obtain food products. Various bacterial compositions are widely used to increase the microbial activity of the soil, which increases the availability of nutrients that plants can easily absorb. They increase soil fertility by fixing nitrogen and dissolve insoluble phosphates in the soil, resulting in the formation of chemicals that promote plant growth. The aim of this work was to determine the presence of phytase, nitrogenase and alkaline phosphatase enzymes in strains of candidate bacteria of bacterial composition by proteomic profiling in polyacrylamide gel. The molecular weights of phytase, nitrogenase and phosphotase of candidate strains were determined in the system http://molbiol.ru/based on amino acid sequences. After that, proteins with the corresponding molecular weights were detected on the forogram. According to the results of proteomic analysis of Bacillus subtilis, proteins with molecular weights of 50.5, 16.5 and 74.9 kDa corresponding to the enzymes phytase, nitrogenase and phosphatase were identified in 10 strains. Proteins with molecular weights of 41.8, 16.7 and 24.8 kDa corresponding to the enzymes phytase, nitrogenase and phosphatase were detected in 6 strains of Bacillus megaterium. 4 strains of Pseudomonas stutzeri contained proteins with molecular weights of 69, 31.8 and 73.5 kDa corresponding to the required enzymes. As a result of the carried out experiments, candidates bacterial strains were identified for the development of a biocomposition to increase the coefficient of assimilation of mineral components of fertilizers.