siRNA-INDUCED GENE KNOCKDOWN IN MAMMALIAN AND HUMAN PRIMARY CELLS
Small interfering RNAs (siRNA) are used for specific inhibition of gene expression in mammalian and human cells of various origins. The use of siRNA for the therapy of certain infectious diseases is promising. To date, the main obstacle to the use of gene knockdown in medical practice is the insufficient effectiveness and safety of the method for primary mammalian and human cells. This review presents current views on the methods of siRNA transfection in primary mammalian cells, their advantages and limitations. The results of our own studies on the possibility of siRNA lipofection in primary immunocompetent cells of human blood are presented. The main principles for the selection of nucleotide sequences of siRNA are described, which ensure an increase in efficiency and a decrease in side effects of gene knockdown.