Effect of supercritical carbon dioxide on the in vivo biocompatible and resorptive properties of tissue-specific scaffolds from decellularized pig liver fragments
The creation of bioengineered tissue/organ equivalents is closely related to the development of biodegradable, highly porous 3D scaffolds, which to some extent provide the microenvironment necessary to maintain the viability of the cellular component. According to many researchers, the most interesting are tissue-specific matrices that can selectively support the adhesion, proliferation and differentiation of tissue cells of those organs from which they are obtained by decellularization. It was shown that during intramuscular implantation in rats of decellularized pig liver fragments (DLFp), independent of the method of detergent residues removing (96 hours of washing in phosphate-salt buffer (PBS) or combined: 24 hours in PBS and 8 hours with supercritical CO<sub>2</sub> (sc-CO<sub>2</sub>), the samples meet the requirements for medical devices in terms of local and general toxic effects. Thus, the use of sc-CO<sub>2</sub> made it possible to reduce the duration of the technology for producing biocompatible tissue-specific matrices based on DLFp by 3 times. Moreover, when using sc-CO<sub>2</sub> at the stage of washing the DLFp matrix, a “mild reaction” of the tissue to the sample is observed during 2 months of intramuscular implantation of the matrix in rats with its complete resorption after 3 months of the experiment. Under the same conditions, the duration of a similar local action of DLFp washed in the PBS on the tissue is 3 months with degradation of 63 % of the matrix of the sample size.