Analysis of Cytotoxic Effects of Medical Gas-Discharge Devices
The aim of the investigation was to analyze cytotoxic effects of various gas-discharge technologies. Materials and Methods. The following gas-discharge devices were used in the work: Pilimin series IR-1, Ir-10 and Brig - radiation of spark discharge plasma; DBK-9 low-pressure mercury lamp - ultraviolet radiation; Corona device for darsonvalization - silent electric corona discharge. The objects of investigation were erythrocytes of Wistar rats, and lymphoid cells of Pliss lymphosarcoma. Results. All examined gas-discharge devices were established to possess membranotoxic and cytotoxic effects. The total number of erythrocytes and lymphoid cells diminished, while the number of nonviable cells grew with the increase of exposure time. Erythrocyte membrane was least resistant to the radiation of the spark discharge plasma with 1,500 μs pulse duration and to ultraviolet radiation of the mercury lamp, whereas erythrocytes were less resistant to the radiation by the gas-discharge devices than lymphoid cells. Pliss lymphosarcoma cells appeared to be more sensitive to the action of spark discharge plasma and corona discharge with 1-10 μs pulse duration. Radiation of spark discharge plasma with 150 μs pulse duration exhibited equally membranotoxic and cytotoxic effect. Conclusion. The results of assessing cytotoxic effect of various gas-discharge devices help to determine the direction of investigations when mechanisms of action of gas-discharge technologies are explored, and to recommend, in particular, a more profound study of such parameter as pulse discharge duration.