Association of the PAI-1 polymorphism and Factor V Leiden mutation with genetic predisposition to myocardial infarction
The role of Factor V Leiden mutation and polymorphism in genetic predisposition to myocardial infarction was investigated. The molecular-genetic method was used. DNA extracted from dried bloodspots was used as matrix for PCR. Distribution of 4G/5G alleles of PAI-1 gene was determined in the group of patients with myocardial infarction in comparison with the control group. It was shown that 4G/4G genotype is a risk factor and the 5G/5G genotype on the contrary is a protective one. The frequency of Factor V Leiden mutation in patients with myocardial infarction was revealed to be 2,3 times as high as in the control group. The obtained data show this mutation is the risk factor of myocardial infarction.