Comparative assessment of morphological changes in myocardium in experimental hypothyroidism in dynamics
The work was performed on experimental data. An experimental model of hypothyroidism by surgery thyroidectomy obtained on laboratory animals, 76 white male rats. The operation was conducted under General anesthesia. In the postoperative period rats were withdrawn from the experiment on the 7th, 14th, 21st, 28th and 45th day. For histological examination took pieces of myocardium of the left ventricle of the heart. As a control used 26 rats a thyroidectomy performed was not performed. Pieces of myocardium were fixed in 10 % buffered formalin and prepared slices according to standard methods. Used staining with hematoxylin and eosin, pikrofuksin by van Gieson, Mallory, by toluidine blue. Immunohistochemistry was performed immunoperoxidase indirect method using biomarkers for p-53 and myoglobin. On the 7th day of the experiment in the myocardium discovered the plethora of vessels, edema and stasis perivenular. On 14th day on the background of edema and vascular disorders appeared places with signs and hydropic degeneration of kardiomiotsitov. On the 21st day the intensity of edema and vascular disorders increased, as well as the development of diffuse hydropic and balloon degeneration with the formation of foci of miotsitolizom. On the 28th day of dystrophic and destructive changes are propagated throughout the myocardium, is observed fragmentation of muscle fibers. 45 days in the myocardium of rats developed marked diffuse edema, vascular disorders, and severe destructive changes in the type of miotsitolizom and fragmentation of fibers. Immunohistochemical study observed an increase in expression of the biomarker of apoptosis p-53. Expression of the marker myogenin increases in the beginning of the experiment (7-14 days) and decreases to 28-45 days.