COPING WITH NONSPECIFIC INTERACTIONS IN COMPLEX NON-INSTRUMENTAL DIAGNOSTICS
Blocking properties of various reagents were analyzed as applied in test-systems to determine antibodies against Bordetella pertussis and tetanus and diphtheria toxoids. Analytic systems have been designed as non-instrumental solid-phase dot-immunoassay using immunosorbents in the form of discs and comb-like fixed strips and carbon conjugate as diagnosticum. Results obtained evidence for possible substantial simplification of nonspecific background elimination when applying nonionogenic detergent Tween-20 that prohibits the nonspecific protein surface sorption on immunosorbent and does not reduce the analytic sensitivity. The procedure characteristics of the process have been determined.