Translational study: methods of mitochondrial dysfunction diagnostics in patients with cardiovascular pathology
The aim of the research. To estimate the prognostic properties of changes in the concentration of mitochondrial DNA (mtDNA) in the blood plasma of patients with cardiovascular pathology in relation to the course of the disease and the effectiveness of the therapy. Material and methods. The study involved patients with coronary heart disease (CHD) with stable and unstable atherosclerotic plaques, signed informed consent for the processing of the data. Patients were hospitalized for examination and treatment of IHD in the cardiology department of the Hospital INC SB RAS. Laboratory and instrumental examination and analysis of the level of free circulating mitochondrial DNA of blood serum was carried out by real-time PCR (copies / ml). The results of hospitalization, assessed as satisfactory, are compared with the level of mtDNA before and after treatment. Results. The mean value of the mtDNA level before and after treatment in cardiac patients is significantly different - 1093686 copies / ml against 418046 copies / ml, respectively (p = 0.02). Unlike women, the level of mtDNA in men is statistically significant (p = 0.03) decreases after treatment. Statistically significant differences in mtDNA levels before and after treatment were found depending on the series of determinations (p = 0.0010) according to the Kruskal-Wallis rank test. Conclusion. The results of the proposed research will allow to identify prognostic factors of cell damage and plaque destabilization in endothelial dysfunction, atherosclerosis and its complications, to conduct clinical approbation of the method of predicting cellular injuries in chronic ischemia against the background of atherosclerosis.