POSSIBILITIES OF GELATIN-GLUTAR SCAFFOLDES USING FOR CULTIVATION OF DERMAL FIBROBLASTS FOR TISSUE ENGINEERING FOR TREATMENT OF BURN INJURIES
The aim of the research. To study the possibilities of fibroblasts cultivation on a 3D-gelatin scaffold with addition of dihydroquercetin. Materials and methods. We described a method of salt leaching of a gelatin-glutar scaffold, and its adhesive properties for cultures of dermal fibroblasts. Results. Rats’ skin fibroblasts showed high adhesion to the resulting scaffolds. We have demonstrated the morphological characteristics of cultured fibroblasts on a scaffold, as well as the ability to modify the matrix, with the appearance on the surface of scaffolds of proteins, which are the product serving as the basis for remodeling of the microenvironment. Conclusion. A method has been developed for the production of gelatin-glutar scaffolds for the cultivation of dermal fibroblasts for tissue engineering, including the reparation of the skin after burn injuries.