Evaluation of processed lipoaspirate cells autografting for the treatment of advanced periodontitis and features of granulation tissue growth
. In the present study were demonstrated favorable results on the growth of clinical attachment level using autological processed lipoaspirate cells at advanced periodontitis. Immunohistochemistry using a monoclonal antibody to Ki-67 showed the lack of mitosis in new tissue cells without significant differences in research groups. Cytological analysis in an initial stage of regeneration process has shown that granulation tissue growth realized due to transformation of the neutrophils, which populates periodontal defects intensively. In an exudative stage wound neutrophils participate in formation of extracellular traps (NETs) for tissue pathogens, in a dehydration stage consecutive neutrophil transformation in connective tissue cells is marked. Grafted stromal cells (MMSC) are capable to be rapidly differentiated at once in precursors of fibroblasts therefore high clinical efficacy of treatment is achieved.