Immunophenotype and functional activity of mucosal immune system cells under different methods of application of the opportunistic bacterial antigens
The features of mucosal and system immunity (changes in immunophenotype, Toll-like receptors expression), cytotoxity and localization of different lymphocyte populations in lymphoid organs after different (intranasal, per os, subcutaneous) methods of immunization with use of polycomponent Immunovac-VP-4 consisting of the antigens of opportunistic bacteria were been investigated. All methods of antigens application revealed the activation of key effectors of mucosal immunity - Tγδ- and B<sub>l</sub>- lymphocytes and CD3<sup>+</sup>, CD4<sup>+</sup>, CD8<sup>+</sup> lymphocytes populations, expression of Class I and Class II MHC molecules. Oral application of antigens led to significant increase of natural killer cells, that correlated with the maximal cytotoxity of intestine mononuclear cells. Mucosal immunization was shown to activate not only local but system immunity also that is been confirmed by the protection of mice after challenge with virulent strains of Klebsiella pneumoniae, Proteus vulgaris and Streptococcus pneumoniae.