DEVELOPMENT AND EVALUATION OF TOXIC PROPERTIES OF OZONATED EMULSION BASED ON FISH OIL AND GINODIXIN
Providing the population with dairy products depends on the development of agricultural enterprises with high profitability of milk production and increase of livestock herds and their and productivity. However, one of the main problems is low reproductive ability of cows because of high sickness rate of endometritis caused by different microorganisms in the uterus and their resistance to antimicrobial drugs. Therefore, the aim of this work was to develop an ozonized emulsion based on Ginodixin and fish oil and to study its acute and chronic toxicity. Materials and methods: the development of novel emulsion included the selection of the optimum ratio of fish oil and Ginodixin, saturation of ozone-oxygen mixture, evaluation of antimicrobial properties against Escherichia coli(ATCC No. 25922 (F-52)) and Staphylococcus aureus(ATCC No. 25923 (F-49))in a concentration of from 1х10<sup>2</sup> to 1х10<sup>9</sup> microbial cells per ml. Ozonized emulsion was evaluated by dividing the counts into acute and chronic toxicity. To evaluate acute toxicity of 0.5 ml of dilution with ozonized emulsion (1:1, 1:2, 1:4, 1:8) was injected intraperitoneally into mature laboratory mice. The maximum dose of the emulsion was 8000 μ/kg and the minimum was 2000 μ/kg. Acute toxic action of ozonized emulsion was evaluated using experimental animals according to clinical sign of intoxication and survival rate of animals which depended on dosage rate of the injected emulsion. Chronic toxicity of ozonized emulsion evaluated using three groups of mice. Results of investigation: ozonized emulsion showed its maximum antimicrobial activity at content of one part of fish oil with 3 - 4 parts of Ginodixin. A single intraperitoneally introduction of ozonized emulsion at maximal dosage of 8000 ц/kg caused intoxication and death in 20% of animals. Dosage of 6000 ц/kg induced unformed fecal masses in laboratory animals and dosages of 4000 and 2000 ц/kg did not reflected in clinical state of the laboratory mice. Conclusion: at intraparenteral introduction of ozonized emulsion maximal tolerated dose for mice was 4000 ц/kg, toxic dose - 6000 ц/kg, and LD<sub>20</sub> - 8000 ц/kg, LD<sub>50</sub> more than 1000 ц/kg accordingly. Ozonized emulsion consisted from fish oil and ginodixin causes destruction of microorganisms present in uterus at inflammation. According to the State standard 12.1.007-76 the ozonized emulsion belongs to the 4th class of toxicity and is characterized as low-toxic. A single introduction of ozonized emulsion to into uterus of cow endometritis weighing 600 kg at acute endometritis the dosage should not be higher than 187.2 ml.