THE IMPACT OF POLYOXIDONIUM ON A SUBPOPULATION INTRATUMORALE LYMPHOCYTES IN PATIENTS WITH BREAST CANCER
The work presents the results of studying the impact of polyoxidonium, used in 20 patients with breast cancer in neoadjuvant mode, on a subpopulation intratumoral lymphocytes. The lymphoma cells studied in the gate CD45 on the material of the core-biopsy (before application of polyoxidonium) and then on the material of the removed tumor (after the application of polyoxidonium). For the painting of the cells used two 4-color samples monoclonal antibodies: CD45/CD3/CD4/CD25 and CD45/CD3/CD8/CD56, the following analysis was performed by flow cytometry. The average number of subpopulations most intratumoral lymphocytes did not change after immunotherapy, authentically decreased only NK-cells (CD3-CD56 <sup>+</sup>), but their content was extremely low: 1,68±0.3% and 1.16 ą 0,3% ( p <0.05). Subpopulation structure of intratumoral lymphocytes 6 patients with the presence of disease variability of tumor tissue after application of polyoxidonium differed significantly higher levels of CD4 <sup>+</sup> lymphocytes: 50,9±2,0% and 42.1±2,0%, p=0.009.