Study of Influence of Surfactant Content on Antioxidant Activity of the Suppositories with Cranberry Oil
Recently hemorrhoid has been reporting as one of the most widespread human diseases and the most frequent reason for seeing a doctor-coloproctologist. Pharmacotherapy of hemorrhoid, which usually is occurred in combination with other anorectal pathologies, is carried out using etiotropic medications and semisolid dosage forms, in particular suppositories, which are considered the most effective between them. Cranberry oil has expressed antioxidative, hypocholesterolemic and antisclerotic activities, and so investigations for development of composition and technology of the rectal dosage form with it for therapy of destructive and inflammatory diseases of rectum are carried out on the department of pharmacy of Ivano-Frankivsk National Medical University. As a result of preliminary biopharmaceutical investigations it was revealed that a composition of confectionary solid butter and emulsifier №1 had provided with the optimal releasing of the active substances of cranberry oil. The aim of this work is scientific substantiation of the optimal content of surfactants in the rectal dosage form with cranberry oil.Suppositories were made by fusion molding method. Concentration of cranberry oil was 0,5%, 1%, 2%, 3% і 5% from a mass of the dosage form, cranberry oil was 0,25 g in the each suppository. Study of scientific substantiation of the surfactant content in the suppositories was carried out according to the plan of single-factor analysis of variance with repeated observations. Releasing of active substances of cranberry oil from the suppositories was chosen as a parameter of optimisation, and it was established using the estimation of antioxidative activity on the model of non-enzymatic initiation of free radical oxidation.As a result of carried out investigation of the suppository with cranberry oil it was established that the surfactant content made a significant influence on antioxidative activity of this rectal dosage form. It was revealed that 0,5% emulsifier №1 provided with the optimal level of releasing of cranberry oil active substances from the rectal suppositories.