Formation of Callus and Induction of Somatic Embryos <i>in vitro </i>Culture in <i>Pinus sibirica </i>Du Tour
In culture in vitro nonembriogenic callus was inducted from immature zygotic embryos of seeds of Siberian pine trees (Pinus sibirica), growing in natural stands of West Sayan and in a clonal plantation. The active callus development goes in explants hybrid seeds, getting as results of control pollination of clones by pollen of Siberian pine trees with annual cycle development of female cones. Cytological study shows that the strong elongation in hypocotyl cells goes at the stage of callus induction. Only explants of embryo seeds of some clones, which were pollination of pollen trees-accelerate reached the stage of proliferation and formed embrygenic mass. Globules of somatic embryos and embryonal tubes are developed from embryonal initiales.