MARKERS OF OXIDATIVE STRESS IN PREDICTING BONE RESORPTION IN A MODEL OF GENERALIZED OSTEOPOROSIS
Based on current information on the pathogenesis of generalized osteoporosis, it becomes relevant to use a va-riety of research methods, including biochemical ones, for the early diagnosis of bone loss. It is known that under oxi-dative stress, the processes of bone tissue resorption are enhanced, the markers of the latter are indicators of oxidative-ly modified proteins. It can be assumed that some of them are biochemically substantiated as reliable signs of the de-velopment of osteoporotic disorders. Aim - evaluation of the possibility of using ketone-dinitrophinylhydrozone as a marker of bone resorption in experimental hypoestrogen-induced osteoporosis. All experiments in this research work were carried out on female Wistar white rats. Osteoporosis was modeled by bilateral oophorectomy. To confirm the hypothesis put forward, the following studies were used: digital radiological densitometry, histomorphometry of bone biomaterial, and to determine the severity of oxidative stress, the values of total and neutral ketone-dinitrophinylhydrozone were biochemically measured. Evidence has been obtained that, after oophorectomy, osteoporotic disorders develop in experi-mental animals, consisting of decrease in bone mineral density in the proximal metaphyses of the humerus and femur and thinning of the width of the bone trabeculae in these localizations, which are accompanied by the development of the oxidative stress phenomenon. The analysis of the results obtained revealed a correlation between the above indica-tors in the norm and on the pathology model.