INFLUENCE OF "PRODEEVIT-TETRA" AND "NANOVIT" PREPARATIONS ON THE ORGANISM OF DRY COWS
Introduction. Peroxide damage of the protein substances leads to their degradation and formation of toxic fragments, including middle-mass molecules (MSM). The accumulation of MSMis a marker of endotoxication, while MSM as toxins complicate the pathological process and affect the vital functions of organs and systems of the body.It has been established that MSM affects the smooth muscle tonus of organs and transvascular transport. Also, MSM can penetrate the placental barrier and thus toxins affect the fetus, which leads to the development of multiple organ lesions in the finalThe goal of the work. Determine the level of endogenous intoxication of the cows’ organism during the dry period due to the effect of preparations Prodevit-tetra and Nanovit.Materials and methods. Experiment was conducted in LLC “Agroprodservice invest” of Ternopil oblast in dairy cattle of Ukrainian black and white breed. Biochemical studies conducted in the laboratory of veterinary obstetrics and gynecology of Ternopil Research Station of the Institute of Veterinary Medicine of the NAAS.The experiment was conducted in cows in 25-35 days before calving. The experimental and control groups of cows were formed (n = 10) for this purpose.Cows of the experimental group in 25-35 days before calving, were intramuscularly injectedml of vitamin complex “Nanovit” (composition: vitamins A, D, E and nanoparticles Cu, Zn, Mn, Co).At this time cows of the control group were intramuscularly administered 15.0 ml of the preparation “Prodevit-tetra” (1 ml of the preparation contains vitamin A-50000 IU, vitamin D- 25000 IU, vitamin E - 20 mg, vitamin F - 6 mg).Results of research and discussion. The concentration of MSM in the blood of cows after the introduction of vitamin preparations and the content of nano-metals, respectively, decreased by 41,0% (p≤0,05) and 61,0% (p≤0,001), which indicates the positive effect of preparations on the cows’ organism.An additional confirmation of the positive effect of “Prodevit-tetra” and “Nanovit” on the cows is, correspondingly, an increase in the antioxidant-pro-oxidant index in 1.7 and 2.3 times (p≤ 0.001) compared with the indices on the beginning of the experiment.After the “Nanovit” application service period of cows decreased by 54 days, and the index of insemination decreased by 0.2 units against the cows, which were introduced “Prodevit-tetra”, indicating the positive effect of the preparations containing the nanoparticles of Cu, Zn, Mn, Co metals and vitamins A, D, E during the dry period in cows.Conclusions and recommendations for further research. After application of “Prodevit- tetra” and “Nanovit” preparations, the concentration of MSM in the cows’ organism is reduced by 41.0% (p≤0.05) and by 61.0% (p≤ 0.001), respectively, while the antioxidant- the pro-oxidant index increased in 1.7 and 2.3 times (p≤ 0.001) compared to the values prior the administration.The preparation “Nanovit”, introduced to cows during the dry period, reduces the service period by 54 days, and reduces the insemination index by 0.2 units against the cows, which were administrated “Prodevit-tetra”.The prospect for further research is to study the humoral level of the immune system of the cows’ organism the action of the preparation “Nanovit”.