RAPID DIAGNOSTICS OF ACUTE INTESTINAL VIRAL INFECTIONS IN MILITARY PERSONNEL
Fecal extracts from patients with acute intestinal infections of unknown etiology from the Western and Eastern military districts were taken as the study material. The aim of the work was to assess the possibility of using rapid diagnostic methods for acute intestinal infectious diseases of viral etiology (rotavirus, adenovirus, norovirus and enterovirus infections) in military personnel. Diagnostics was performed using the latex agglutination reaction and the immunochromatographic analysis method. Studies on the effectiveness of virus concentration on filter membranes were performed using the HRV/SPb human rotavirus model/884/10/05 from the collection of the Federal state budgetary institution "scientific research Institute of influenza" of the Ministry of health of the Russian Federation. Viruses were isolated on MA104 culture and passed 5 passages. For testing regimes of electron-microscopic studies, which were used when working with rotaviruses, noroviruses used the human from the collection of fsbi "Sri of influenza" Ministry of health of the Russian Federation. Noroviruses were isolated from clinical samples of feces of sick people in Saint Petersburg. Norovirus concentrate was obtained by multi-stage purification using centrifugation and membrane filtration in a tangential flow. The study resulted in conclusions about the effective use of these methods for rapid diagnosis of acute intestinal infections of viral etiology.