COMPARISON OF ASSESSMENT OF THE MEMBRANE INTEGRITY STALLIONSPERM USING OF DIFFERENT METHODS
In order to reveal the most efficient method of determining the membrane integrity of stallion semen, we carried out a comparative evaluation of three different methods of determining this indicator: staining with eosin, staining with fluorochrome (SYBR-14 + PI) and Hypo-osmotic test. The stallion semen showed high indicators of correlation of sperm motility with percentage of intact membranes by all methods of determination. The greatest coincidence was shown by the eosin staining method with a correlation coefficient of 0.91. For correct evaluation, it is necessary to subtract the number of unpainted pathological sperms that are not functional from the number of unpainted sperms. The percentage of mobile sperms does not coincide with the percentage of whole membranes in the process of storing of stallions sperm by all methods of determination. With complete loss of mobility, a significant number of cells remain with intact membranes at the end of storage. This shows that the stoppage of sperm occurs due to the expenditure of energy material in the mitochondria of the flagellum, and has no connection with the stability of the membranes. Therefore, it is advisable to evaluate the membranes of stallion sperm immediately in freshly obtained or thawed sperm. This study showed that the determination of membrane integrity is desirable to conduct to evaluate the mobility, especially in doubtful cases. For semen, the most preferred method of staining sperm is with eosin, which is characterized by simplicity, availability and cost effectiveness.