Contribution of intergenic interactions of polymorphic variants of candidate genes to the development of a gastric ulcer
Introduction: Peptic ulcer disease occurs in 5-10% of the adult population, and is characterized by a high percentage of complications, which is a serious medical and social problem. The contribution of hereditary factors to the etiopathogenesis of the disease leaves 5.5-50%. The aim of the study was to study the contribution of intergenic interactions of polymorphic variants of candidate genes (rs2294008, rs505922, rs6136, rs8176720, rs2519093, rs507666, rs651007, rs579459, rs649129) to the development of gastric ulcer (GU). Materials and methods: The sample consisted of 217 patients with GU and 347 individuals from the control group, the regulatory potential of polymorphic loci were evaluated using the online databases, and genotyping was performed by PCR. The study of SNP×SNP interactions of polymorphic variants of candidate genes associated with the development of GU was carried out using a modification of the MDR (Multifactor Dimensionality Reduction) - Model-Based-MDR (MB-MDR) method, data visualization was carried out in the form of a dendrogram and graph using MDR software (v. 3.0.2). Results: All 9 studied SNPs as part of 10 significant models of interlocus interactions are involved in the formation of GU. The largest number of models includes rs8176720 of the ABO gene and rs2294008 of the PSCA gene. These polymorphic variants have a pronounced regulatory potential in many organs (tissues), incl. in the target organ of GU (stomach).