Genetic markers of immune response disorders in women of reproductive age with acute pyelonephritis
Acute pyelonephritis (AP) is an inflammatory and infectious process that occurs due to immunological disorders, occurring mostly in the calyx-pelvic renal system and the tubulointerstitial zone. Pyelonephritis more often occurs in women of reproductive age. THE AIM: to study genetically determined changes in the Toll-like receptor 4, mutations in the genes of interleukin 1 ß, interleukin 6 in women of reproductive age with acute pyelonephritis. PATIENTS AND METHODS: The study included 26 women with acute pyelonephritis. Control group consisted of 27 practically healthy women comparable ages and without infections of kidneys and urinary tract in anamnsis. The presence of polymorphism of T (-31) C genes IL-1 ß, C (-174) G IL-6 and Arg299Gly TLR4 was revealed by PCR. RESULTS: Women of reproductive age with acute pyelonephritis have a high prevalence of polymorphism of IL-1 ß and TLR4 genes. Genotypes of TT and CT and also polymorphism of the T (-31) C gene of IL-1 ß and the genotype of the GG polymorphism of the gene Asp299Gly TLR-4 were associated with an increased risk of inflammatory infectious process in the kidneys. In healthy individuals, CC genotypes are found for the T (-31) C gene of IL-1 ß and AA for the Asp299Gly TLR4 gene. CONCLUSIONS: It is useful for women of reproductive age to provide genetic diagnostics to detect reference genes polymorphism. It will allow to reveal AP development risk group for carrying out primary prevention.