Immunoblotting in the diagnostics of the syphilis initial stages
After the observation of 210 patients with syphilitic infections' early forms the blood serum's samples were investigated in the complex of standard serological reactions for the diagnostics of syphilis (RMP, CAA(complement-activation assay), EMI(enzyme multiplied immunoassay), PHR (passiv hemagglutination reaction), ITabs (immunofluorescence test with absorption) and in the immunoblotting with the set of reagents (INNO-Lia™ Syphilis Score, firms INNOGENETICS NV, Belgium). The heightened sensibility of the lineal immunoblotting had been stated after diagnostics of the initial (100 %), secondary (100 %), and early symptom-free syphilis (100 %), observation of the persons, who had sexual intercourse with the syphilis patient (36 %). Sensibility indices of the immunoblotting exceeded the sensibility of the standard non-treponema (RMP) and treponema (EMI, PHR) tests by the initial and early symptom-free syphilis. At the same time the investigation sensibility using immunoblotting and ITabs by the initial syphilis were maximal (100 %). Diagnostic specificity amounted to 98 %.