THE INFLUENCE OF PGKP-135 ON CULTURAL PROPERTIES OF PATHOGENIC STRAINS OF M. BOVIS
The aim was to study the effect of different concentrations of PGKP-135 and isoniazid on the cultivation of pathogenic strains of M. bovis at 37 °C and various pH. The studies were conducted in the laboratory of the Department of Epizootology and Infectious Diseases of Animals of Veterinary Medicine Faculty of Dnepropetrovsk State Agrarian and Economycs University during the 2015-2016 biennium. The cultivation and accumulation of biomass of the strains of M. bovis was performed on egg medium, which composition was identical to the standard, manufactured by GP “Veterinary Medicine” (Kharkiv, Ukraine). Drugs PGKP-135 or isoniazid were added to the medium in three concentrations (0.1; 0.5;1 %). The studies were conducted with the establishment of two levels of pH 6.5 and 7.1. At the beginning of the experiment the effect of drug concentration and pH on the growth rate of the culture at 37 °C was adjusted. M bovis of 100 passage, which was cultivated at 37 °C trough the medium with PGKP-135 drug at indicated concentrations in a thermostat for three months at pH of 6.5 and 7.1 (in an amount of ten tubes for each drug concentration) was collected. M. bovis of 100 passage without PGKP-135 was used as a reference.After completion of these terms as well as after cultivation at 37 °C the accounting and characterization of colony growth of mycobacteria. was carried out. As a result, data concerning on the PGKP-135 and isoniazid concentrations influence on the M. bovis cultivation at 37 °C in media with different pH were obtained.For all studied (0.1; 0.5 and 1.0 %) concentrations PGKP-135 the lack of growth of the pathogenic strain of M. bovis of 100 passage was noted during the observation period (90 days). The drug PGKP-135 actively influences the properties of the culture of a pathogenic strain of M. bovis, cultured in a medium with a pH of 6.5 and 7.1 at 37 °C, holds back growth and development, shows tuberkulostatic action. Low concentrations (0.1 and 0.5 %) of the isoniazid in the medium with pH 6.5 and 7.1 at 37 °C does not restrain the growth of a pathogenic strain of M. bovis of 10 passage.