Microbial synthesis of exopolysaccharide ethapolan on various types of waste vegetable oils
The research on the use of industrial waste to obtain practically valuable microbial metabolites was intensified. Oil-containing waste are cheap and available in necessary for use in microbial technologies quantities. Nevertheless in the literature there are only a few reports about the possibility of their application as substrates for the biosynthesis of microbial polysaccharides. To investigate the synthesis of exopolysaccharide eрtapolan (produser - Acinetobacter sp. IMV B-7005) on waste (fried) oil of various qualities (with different ratios of mono- and polyunsaturated fatty acids). It was established that the highest ethapolan concentration (11-14 g/l) was observed under Acinetobacter sp. IMV B-7005 cultivation on waste after frying meat sunflower and corn oils at concentration 5 %, with using inoculum grown on refined oils. Replacing these oils in the cultivation medium on olive and rapeseed accompanied by some decrease in EPS concentrations (to 9-10 g/l), the EPS-synthesizing ability was higher in several times (6.3-7.6 g EPS/ g biomass). The possibility of exopolysaccharide ethapolan biosynthesis on waste vegetable oils, characterized by a high content of polyunsaturated (sunflower, corn) and monounsaturated (olive, rapeseed) fatty acids was shown.