Improving the effectiveness of modern sorbents
The aim of the study was to determine in vitro the sorption capacity of the following sorbents: Polyfepan, Perlite, Vermiculite, and to develop a comlex drug based on the abovementioned sorbents. Analysis of mycotoxins was performed using method of enzyme immunoassay -(ELISA) on "Agra Quant" set. Working solutions of mycotoxins were prepared by dissolving dry crystalline mycotoxin standards produced by "Sigma" and "Biopure". Practical efficiency indicator (net efficiency) was accepted for quantitative characteristics of sorption capacity. Sorbent net efficiency is determined in percentage as the difference between adsorption and desorption. Mycotoxin sorption is measured at different pH, simulating the change of acidity in the digestive tract of animals. The amount of adsorption and desorption (in %) is measured at production test in vitro. Set of sorbents: Vermiculite, Perlite and Polifepan taken in equal shares had a pronounced sorption activity in relation to mycotoxin T-2 and amounted to 95%. Desorption net efficiency comprised 95%. Sorption capacity of Vermiculite, Polifepan and Perlite taken together for T-2 toxin was significantly higher than the sorption capacity of each drug taken separately. Adsorption activity of the most active against T-2 sorbent comprised 87%, which is 8% less than that of the complex preparation. Addition of sodium thiosulfate into the sorbent complex enhanced the sorption capacity against T-2 mycotoxin by 5%, net efficiency being 100%. In relation to deoxynivalenol sorption activity was 21%, desorption was 17%. T-2 and DON belong to mycotoxins difficult for adsorption, due to their low polarity, and the fact that today's sorbents are polar. Therefore, it is reasonable to use complex of sorbents when treating and preventing T-2 and DON mycotoxicoses. Adsorption activity of sorbents complex is significantly higher than the sorption activity of each sorbent used separately.