The role of reactive oxygen species and nitric oxide in the realization of the adaptation mechanisms of bone-derived multipotent mesenchymal stromal cells to hypoxia under cultivation with growth factor bFGF
The simulation of conditions in vivo under cultivation the stem cells in vitro as well gas media content (low O<sub>2</sub> concentrate) and different growth factors presence is one of the feasible way of growth rate of multipotent mesenchymal stromal cells (MMSC) culture increase when kept differentiation potential and maintained high viability. Oxygen metabolites: reactive oxygen (ROS) and nitrogen species are the most interesting for studying the influence of above characteristics on proliferation activity and viability of MMSC So it is currently important to identify ROS and NO role in MMSC by cooperative cultivation with growth factor bFGF under hypoxia. The research was aimed to study an effect of 5% hypoxia on Н<sub>2</sub>О<sub>2</sub>, О<sub>2</sub><sup>-</sup> and NO content in MMSC from bone marrow in the cell culture in the presence or absence bFGF It was found out that both 5% hypoxia and bFGF (7 ng/ml) decrease the intracellular Н<sub>2</sub>О<sub>2</sub>, О<sub>2</sub><sup>-</sup> and NO concentration on the background of elevated HIF1α gene expression and depressed p53 gene expression. At the same time simultaneous action of hypoxia and bFGF promotes minimal ROS and NO generation, maximizes the effects on HIF1α and p53 genes expression probable for the genome protection Taking into account the increasing the proliferative activity and viability of MMSC culture under these conditions that was shown previously by us, obtained results indicate the regulatory role of ROS and NO in the long-term MMSC adaptation to 5% hypoxia, simulating O<sub>2</sub> physiologic content as in vivo