EFFECT OF 3-NITROTYROSINE THE DIFFERENTIATION AND FUNCTIONAL ACTIVITY OF DENDRITIC CELLS IN PATIENTS WITH BRONCHIAL ASTHMA
In conditions of nitrosative stress dendritic cells mature in the presence of high concentrations of 3-nitrotyrosine, which can affect their functional activity. The study involved patients with mild and severe bronchial asthma. Cultivation of immature dendritic cells from peripheral blood monocytes was performed by standard protocols in the presence of 3-nitrotyrosine to induce maturation of dendritic cells using lipopolysaccharide. To control the maturation of dendritic cells was evaluated expression of cell markers (CD 25, CD83, CD86, HLA-DR). Established that the 3-nitrotyrosine in concentration 5,10 ng/ml stimulates the maturation of dendritic cells in patients with bronchial asthma. High doses of 3-nitrotyrosine cause a delay of maturation and inhibition of functional activity of dendritic cells