FORMATION OF POLYEMBRYOIDS IN <i> IN-VITRO </i> CULTURE AS A STAGE OF WHEAT CLONING BIOTECHNOLOGY
Our research is devoted to the development of a reliable way to produce polyembryoids in in-vitro anther culture of spring soft wheat. It has been found that the frequency of polyembryoid formation is dictated mainly by the relationship between the concentration of a synthetic auxin (2,4-D) in the induction medium and the content of an endogenous auxin (IAA) in anthers before inoculation. Thus, we have shown the general possibility of stable large-scale production of in-vitro polyembryoids in order to develop cloning methods in valuable wheat genotypes for the purpose of selection.