Viability and growth of black current plantlets (Ribes nigrum) on the different stages of in vitro culture using 2'',5''-olygoadenylates
The viability of black current cv. Pamyat Vavilova in in vitro culture containing antiviral compounds (2',5'-olygoadenylates, virazol) has been investigated. It was found that virazol-derived 2',5'-olygoadenylates did not exert negative influence on the vitality and propagation coefficient of black current regenerants. The long application of virazol in 25 mg/l concentration resulted in a total death of black current microcuttings at the end of second passages. A long-term cultivation of black current regenerants on nutrient medium contained 2',5'-olygoadenylates did not adversely affect the rhyzogenesis processes. The cultivation of microcuttings on nutrient medium with 2',5'-olygoadenylates results in a decrease of average root length and does not diminish the number of roots.