Dynamics of enzyme activity in endogenous structures of the enteral environment in model animals (mini pigs)
The enteral environment plays an important role in maintaining homeostasis as well as in the interaction between the living body and the external environment. High molecular weight compounds that enter with food, and unable to penetrate the intestinal membrane into the internal environment of the body, must be converted to relatively simple molecules. Enzymes of the digestive glands take an active part in this process. We studied the localization sites and the activity of the enzymes gamma-glutamyltransferase and alkaline phosphatase at the level of the enteral environment in model animals (mini-pigs). The activity of enzymes in the mucous membrane, native chyme of the intestine and its fractions, dense endogenous and soluble, were determined. The activity of gamma-glutamyl transferase is found both in the mucous membrane and in the chyme of all parts of the small intestine. The highest activity of gamma-glutamyltransferase was recorded in the soluble fraction of the chyme, the lowest in the dense endogenous fraction with a similar pronounced dynamics of activity along the entire length of the small intestine. The highest activity of alkaline phosphatase was found in mucous overlays of the small intestine. During the evacuation of the chyme, the activity of the enzyme in it decreases. The maximum activity of alkaline phosphatase was recorded in the dense endogenous fraction of the chyme of the small intestine.