The study of mechanisms of accumulation of daunorubicin and rodamin-123 in cells of human venous blood using cytometry technique
The article presents comparative data of cytometry estimation of accumulation of daunorubicin and rodamin-123 in cells of peripheral blood of healthy people under incubation of substances in vitro. It is demonstrated that maximal saturation ofthrombocytes occurs during the first five minutes, of leukocytes during forty five minutes. The erythrocytes factually never accumulate these compounds. The maximal values of accumulation of substances in leukocytes are characterized by high inter-individual variation. The close correlation (Rs=0.96-0.98) of parameters of accumulation of substances in lymphocytes and neutrophils testifies the presence of similar mechanisms of control of activity transportation ofxenobiotics in nucleated cells of blood. However, the results of inhibitor analysis of input of Pgp-dependent mechanisms of accumulation of rodamin-123 by leukocytes differ the data received under application of daunorubicin that reflects differences of their intracellular binding sites. The expressed differences between parameters of accumulation of daunorubicin and rodamin-123 by leukocytes in various patients determine necessity of individual approach in monitoring of development of medicinal resistance.