The influence of ozonized physiological solution on the activity of endocannabinoid system
The purpose of the research is to study the effect of ozonized saline solution effect on the expression of endocannabioid CB <sub>2 </sub>receptors by immune system cells in vivo. 16 healthy volunteers aged 20-42 (30.4±6.2 years) were involved in the study. The intravenous 0.9% NaCl saline solution injection was applied. Solution was ozonated by Medozons-BM, Russia. 200 ml of solution was preliminary ozonated with the concentration of 6 mg/l and further administered intravenously drop-by-drop. The expression of surface markers of endocannabioid receptors on mononuclear leukocytes was determined with the aid of appropriate antibodies (Santa Cruz Biotechnology, the USA). Findings were recorded by the multiparametric flow cytometry method on flow cytometer FACSCalibur (Becton Dickinson, the USA). Data obtained on the inhibition effect of ozonated saline solution to the activity of the expression of endocannabioid CB <sub>2 </sub>receptors disclose new mechanisms for the immune response regulation by ozone and may be used for purposeful action to the immune response by organism via the endocannabioid system.