Morphogenesis of the infectious process caused by associated infection (experimental research)
Aim. To study the microbiocenosis and pathomorphology of animal organs infected with P.aeruginosa and uncultivated forms; to identify the development mechanisms of the infectious process caused by associated infection in combination with the endogenous microflora of the animal organism. Materials and methods. The experimental model was created on rabbits of the Soviet Chinchilla breed, which are sensitive to S.aureus and P.aeruginosa bacteria. 58 rabbits were used in the experiment, divided into III groups: Group I - 26 animals infected with cultured forms of bacteria; group II - 26 animals infected with uncultivated forms of bacteria. Group III included 6 healthy intact animals. Infection was carried out by a suspension of cultured and uncultivated forms of P. aeruginosa and S.aigeis bacteria isolated from patients being treated in the burn department of the Regional Clinical Hospital No. 1 in Tyumen, in the dilution of a bacterial suspension in a concentration of 105-106 microbial cells in 1 ml on a saline solution of sodium chloride. The microbial landscape of internal organs has been studied. Histological, immunohistochemical and morphometric studies of the lungs, liver and kidneys on the images of histopreparations digitized on the histoscanner “MIRAX MIDI” (Carl Zeiss, Germany) using software using the Axio Scope A1 microscope (Carl Zeiss, Germany). Conclusion. In the pathogenesis of the experimental infectious process, a large role belongs not only to primary pathogens, but also to E. coli translocated from the intestine. The translocation of E. coli and its inclusion in the microbial association reflects a decrease in the protective properties of the macroorganism on the one hand and an increase in the role of GR-negative microorganisms, on the other hand. It was revealed that during the development of the infectious process in the body of experimental animals, immunosuppression develops against the background of autoimmune inflammation. Possible cause of early death of the experimental group animals is the development of bacterial shock.