Detection of lipid hydroperoxides content in serum lipoproteins by the microperoxidase-luminol system
The increase of content of lipid peroxidation primary products - lipid hydroperoxides - in human serum apo-B-containing lipoproteins (apo-B LP) can be considered as one of the signs of oxidative stress development. The aim of the investigation was to find optimal experimental conditions for the chemiluminescent detection of lipid hydroperoxides in human serum apo-B LP in the presence of the microperoxidase-luminol system. It was discovered that the kinetic of apo-B LP chemiluminescence in the presence of the microperoxidase-luminol system had a complex mechanism. It was established that «fast flash» of apo-B LP chemiluminescence was connected with destruction of lipid hydroperoxides which are a part of those lipoproteins. The influence of experimental conditions, as well as the initial degree of apo-B LP lipid peroxidation on the chemiluminescence in the microperoxidase-luminol system were investigated. The proposed method can be used to determine the lipid hydroperoxides content in serum apo-B LP.