EXPERIMENTAL STUDY FOR BOTH ASSOCIATED AND ISOLATED INFLUENCE OF RETINOL ACETATE AND Α-TOCOFEROL IN RELATION TO PHYSICAL - CHEMICAL CHARACTERISTICS OF ERYTHROCYTE MEMBRANES BY STRESS TOLERANCE TEST (IN VITRO)
We have carried out experimental research, which included the introduction of oily lipid soluble vitamins into the three equal samples of erythrocyte membrane suspension of practically healthy children (in vitro). Their forms had been calculated in accordance with therapeutic dosages of isolated effect (retinyl acetate and α- tocopherol) and associated (joint) effect (aevit). By the correlation analysis method we have revealed that all the oily lipid soluble vitamins, which had been introduced into the initial suspensions of erythrocyte membranes (in vitro) did provoke primary destabilization changes in erythrocyte membranes in infants. They are shown by molecular conversions in the lipid surface area of the tested erythrocyte membranes and in their integral albumens. The said modifications caused the growth of ion permeability in their surface phospholipid area and the disturbance of calcium energy-transport metabolism. The administration of the preparations of oily lipid soluble vitamins of any form, which were produced pharmacologically (isolated or associated (joint) forms) contributed to both weakening and breaking of weak hydrogen bonds of structured water with the main structural components of biomembranes (albumens and phospholipids), because the mentioned vitamins are intrinsically xenobiotics, that is some foreign substance, which destructs those bonds and contributes to destabilization. These processes are not favorable for the functioning of erythrocyte membranes, especially under the conditions of child's growth and development. Our findings, namely, the patterns, which we marked, call for the necessity to work out the new approaches to the therapy involving the said vitamins as well as to preventive measures, especially in early ages.