Blood brain barrier cell proliferation under HIF-1 suppressed activity in vitro
Aim of the research. To study proliferation and adhesion properties of neuronal and astroglial cells at directed activity modulation HIF-1. Materials and methods. The experiments were performed in the 3-cell BBB model and in isolated neurons and astrocytes in vitro. HIF-1 activity was suppressed by FM19G11 inhibitor, impedimetric cell index was measured with the analyzer «xCelligence». Results. We found that HIF-1 inhibition reduced proliferation and astrocytes and neurons in both the systems. At the same time, the presence in the system of cerebral endothelial cells has stabilizing effect on the proliferative activity of neuronal and astroglial cells. Conclusion. Proliferative properties of astrocytes and neurons have a HIF-1-dependent mechanisms.