Cyanine Dye Modified 2''-Deoxyuridine-5''-Triphosphates: Synthesis, Applications, and Linker Effect on Substrate Properties for Taq DNA-Polymerase
A number of fluorescently labeled nucleotides with electroneutral indodicarbocyanine dye (Cy) at C5 position of the pyrimidine bearing trans-alkene group and with varied linkers have been synthesized. These labeled nucleotides have been tested as substrates for Taq polymerases during PCR using “TB-biochip” test system.