DNA-ﬁngerprinting of sweet cherry cultivars bred in the North-Caucasian
DNA-ﬁngerprinting of sweet cherry cultivars bred in North Caucasian Research Institute of Horticulture and Viticulture was performed based on the analysis of microsatellite loci. The studied varieties are demanded in horticulture in agro-climatic conditions of the North Caucasus and the Kuban and represent breeding interest as sources of useful adaptive traits. To analyze polymorphism of studied set of genotypes 7 SSR-loci were selected: ﬁrst identiﬁed and mapped in the genome of cherries (Ps12a02a, EMPaS02, EMPaS01) and peach (BPPCT002, UDP96-008, BPPCT038, CPPCT006). Presented microsatellite markers were grouped into multiplex sets SSR-markers to analyze several loci simultaneously. Optimal combinations of SSR-markers allowed to obtain interpretable results in the fragment analysis. High level of transferability of SSR markers developed on a peach for genotyping sweet cherry varieties was conﬁ rmed. Thus at created on the basis of microsatellite sequences of a peach markers BPPCT002 and BPPCT038 in the analysis of sample varieties were identiﬁed clear PCR-products conforming to 5 alleles for each locus. The highest level of polymorphism markers developed on a peach had CPPCT006, which had identiﬁ ed 6 alleles. Amount of alleles detected in all studied SSR-loci ranged: 3 to 6 per locus in the set of 14 genotypes. For allinvestigated cultivars unique DNA-proﬁles were detected. Analysis of DNA ﬁngerprints obtained by SSR 7 showed that all studied varieties possess a unique set of alleles. Most polymorphic SSR-markers have been selected for further use in DNA-ﬁngerprinting of genetic resources in culture.