Neuropathic pain syndrome in wistar rats at different stages of chronic brain epileptization (chemical kindling)
Objective. To analyze the features of pain sensitivity in Wistar rats throughout the development of neuropathic pain syndrome (NPS) at different stages of brain epileptization induced by pentylenetetrazole (PTZ). Material and methods. Two series of experiments were carried out according to the same scheme with an interval of one year and different types of anesthesia (series 1 - chloral hydrate, series 2 - ether). NPS was reproduced by intersection of the left sciatic nerve within the popliteal fossa. NBS intensity was assessed in scores considering severity of autotomy on the operated limb (lesion of bones, phalanges and feet) and the number of animals with autotomy. Kindling was performed by daily intraperitoneal administration of PTZ at a subconvulsive dose of 30 mg/kg for 24 and 27 days. Results. NPS at early stages of kindling was similar in both groups (1-2 points). At the later stages of kindling, autotomy was less common in animals with seizure score 4-5 compared to those with seizure score 1-2 and control animals. Conclusion. Animals with seizures score 4-5, subjected to kindling for 24 and 27 days, were more resistant to NPS compared to control animals.