Standardization of buckbean (Menyanthes trifoliata) leaves
By taking into account the contribution of iridoids to the pharmacological effects of the raw material, the authors have developed procedures for the qualitative and quantitative determination of iridoids in the buckbean leaves to update the existing pharmacopeial article for buckbean (Menyanthes trifoliata) leaves. Procedures have been proposed for the thin-layer chromatographic determination of iridoids to identify the buckbean leaves stored in different areas of Siberia. The chromatogram obtained for the test solution should show a grayish or grayish-violet adsorption area at the level of CO loganin adsorption solution area with Rf about 0.43-0.48. To assess the quality of buckbean leaves, the authors have proposed a validated procedure for the spectrophotometric determination of the sum of iridoids calculated with reference to garpagide acetate.