The use of bacterial lipopolysaccharide for pathological processes modeling on biomedical research (literature review)
The bacterial lipopolysaccharide Gram with negative microflora is widely used for modeling various pathological processes in biomedical research. It has the standard toxicological properties and has not only a sample but also a group specificity. There are many different techniques using bacterial lipopoly-saccharide, which greatly complicates the choice of the method of the pathological process modeling and puts quite a difficult task for the researchers to introduce the definition of the multiplicity of doses and routes of administration of lipopolysaccharide. At present, the fundamental in the pathological processes simulation is the duration of lipopolysaccharide administration and the development of acute or chronic toxicity. In the models reproducing the endogenous acute toxicity, a single lipopolysaccharide administration is used within 7 days. A most dose is from 0,2 mg / kg to 15 mg / kg body weight of the experimental animals. Another method of the acute intoxication modeling is to increase the endogenous concentration, so called "physiological" lipopolysaccharide in the systemic circulation. This requires constant monitoring of laboratory parameters, indicating the development of endogenous intoxication. The rate of development of the pathological process is largely determined by the mode of lipopolysaccharide administration, the most commonly are intraperitoneal, intravenous and subcutaneous. However, some researchers use an intracolonic, intratracheal, inhalation and intracranial administration.