TO THE QUESTION OF PRODUCING EXTRACELLULAR MATRIX FRAMES BY PERFUSION DECELLURIZATION
This article describes the first experience of producing extracellular matrix frames from rats' liver in the laboratory the "Institute of biology of aging" (Voronezh). The experiment was performed on 34 Wis-tar male Long-Evans, mass of 300 ± '50 gr. Standard scheme decellurization of liver included 24-hour perfusion of 1% sodium lauryl sulfate (SDS, sodium dodecyl sulfate), prepared on the basis of phosphate buffer supplemented with 0,02% EDTA with periodic replacement of the solution after 1, 8 and 16 hours via a specially designed system comprising rotary pump. After perfusion for purification of vessel channel of body from the agents of decellurization, lytic enzymes and tissue detritus, an organic complex for 15 minutes was perfused with saline. The authors developed and applied in practice the algorithm for estimating the quality decellurization of matrix frames, produced in the laboratory. The algorithm is consistently pursued visual evaluation matrix properties, an objective assessment of histological sections and subsequent immune histochemical studies. It is proved that the provisional freezing of the organic complex perfused by solution extracellular cryoprotectant, has a positive effect on decellurization results. This technique allows to quickly obtaining a high-quality extracellular matrix frames with maximum preservation of matrix glycoproteins.