MORPHOMETRIC CHARACTERISTIC OF RATS PANCREAS AT PRE-SLAUGHTER STRESS UNDER USING OF BIOLOGICALLY ACTIVE SUBSTANCES
We have carried out studies on morphometric parameters of rat pancreas under pre-slaughter stress conditions using biologically active substances of plant and animal origin. The spleen extract, Echinacea and Chinese lemon extracts (70°alcohol solutions), sprouted grains were added to the diet of rats of all experimental groups by aerosol method as an antistressors and immunomodulators in pre-slaughter period (five days before slaughter). Only 70°alcohol solution in the same volume and the same method was added to the diet of rats of control group five days before slaughter. The experiment continued for five days in the vivarium, laboratory animals taken from cage in turn, weighed and decapitated under ether anaesthesia. Significant changes in the morphological structure of pancreatic tissue under pre-slaughter stress have not manifested. At morphometric investigation of the pancreas islets Langerhans diameter of rats of all experimental groups showed some differences. In rats, which were added to the diet spleen extract, the area occupied by the islets of Langerhans, was the largest (I experimental group). The rats of control group, which were not fed with immunomodulators and anti-stress compounds, the signs of the local replacement of the exocrine pancreas, especially in areas around blood vessels, adipose tissue been observed, which were within normal limits. These results obtained in model experiments on laboratory animals in the vivarium can be used in research on agricultural animals to prevent the effect of a pre-slaughter animal stress on the quality of a meat product.