Dynamics of synthesis and circulation of subclasses specific IgG in the immune response to the vaccine to the vaccine against measles, rubella, mumps
The aim of the study was to investigate the dynamics of synthesis and circulation of subclasses of specifi c IgG in the immune response to the Priorix and development of parameters for the separation of primary and secondary types of immune response. The material of the study was the serum samples of children vaccinated against measles, rubella and mumps with Priorix received in 3 weeks after inoculation - 24 children (group 1), in 4 weeks after inoculation - other 26 children (group 2), in 6 weeks - also other 25 children (group 3), 1 year after vaccination - 38 children from the fi rst three groups (group 4), after 5 years (before revaccination) - 24 children from the fourth group (group 5). In the serum samples, the specifi c IgG antibodies and the spectrum of their subclasses against measles, rubella and mumps viruses were studied using the enzyme immunoassay. It was shown that at the beginning of the immune response to all three viruses, IgG3 subclass antibodies were formed, but the rate of growth and switching to a predominantly IgG1-type immune response was diff erent. Cut off was calculated for the antibodies of subclasses IgG1 and IgG3, which were 46.84% and 42.8% for measles virus, respectively; for rubella viruses 62.67% and 12.63% respectively; for the mumps virus 54.97% and 37.26% respectively. The cut off values obtained make it possible to separate the primary and secondary immune response by the ratio of the antibody subclasses. The importance of the developed approach to the separation of early and late type immune response in vaccinated was confi rmed in an independent group of vaccinated children: 16 people 4 weeks after inoculation and 15 children one year after vaccination Priorix, as well as in the study of antibodies in serum from patients with measles adults (14 not previously vaccinated patients and 15 vaccinated in childhood patients).