Identification of viruses, pathogens of viral diseases of grapes
Recently, the number of grapes saplings, infected with viruses, pathogens of viral diseases of grapes has increased. Timely diagnosis of viral diseases and identification of viruses - success in obtaining healthy planting material of grapes in the production of domestic certified planting material of grapes. The purpose of the study was to identify and identify grape viruses in the vineyards of southern Ukraine. To achieve the goal, the following tasks were set: to investigate the infection of vineyards with viral diseases in southern Ukraine; conduct molecular biological diagnostics to identify grape viruses. To perform this work, the following methods were used: ELISA (enzymelinked immunosorbent assay) and RT-PCR (reverse transcriptase polymerase chain reaction) in real time. It has been established that in the vineyards of the south of Ukraine the virus of Grapevine Leaf Roll -Associated Virus of the 1st and 3st serotypes and the Grapevine Fanleaf Virus is progressing. It has been established that the symptoms of viral diseases do not always coincide with the identification of viruses. The dynamics of the spread of various grape viruses depends on climatic conditions, agricultural practices and other conditions and may not coincide with the data on the presence and spread of grape viruses in other countries of Europe and America.