STUDY OF DEPENDENCES OF ANALYTICAL PARAMETERS OF IMMUNOCHROMATOGRAPHIC TEST SYSTEMS FROM THE REGIME OF IMMOBILIZATION OF IMMUNOREAGENTS ON MEMBRANES
The article presents the results of investigation of the influence of deposition conditions of immunoreagents on membrane carriers for analytical performance of immunochromatographic assays. Besides, recommendations for reaching optimal parameters of immunoassays are formulated. The studies were carried out to optimize various parameters of reagents applying onto nitrocellulose membrane, including operating speed and leading of reagents, pH of buffers used for immobilization of proteins, concentrations of salts and detergents introduced into the working buffer for the application, the protein concentration of the applied reagents. Experimental curves were obtained on the example of immunochromatographic test system for the determination of bacterial antigen, lipopolysaccharide (LPS) of Brucella abortus. The given recommendations have common character and may be used for optimization of test-systems for the detection of wide row of antigens.