Wholegenomesequencing ofastrainofStaphylococcus aureuswithatypical properties,isolatedfrom childrenlivinginareas contaminatedbyemissions fromthemetallurgicalplant
Background: bacteria, including Staphylococcus aureus, under the influence of pollutants in the external environment can alter the phenotypic properties. Probably, the basis for such changes is the acquisition of new genetic fragments. Aim: to conduct full-genome sequencing of a strain of S. aureus to study the possible genetic changes associated with anthropogenic impacts of xenobiotics. Materials and methods: the subjects of study were the S. aureus Alex 451 isolated from resident bacteriocarrier living in areas contaminated by emissions from the metallurgical plant. The strain showed resistance to several xenobiotics: compounds of Nickel, chromium, cadmium, manganese, and vanadium pentoxide. Whole genome sequencing performed on a GS Junior sequencer (Roche, Switzerland). Result and discuss: shown that the genome of Staphylococcus aureus Alex 451 differs from the typical representatives of bacteria of this species by the presence of DNA fragments encoding resistance to some metals, and gene of kolicin V. In the genome revealed genes for resistance to several antibiotics, this obviously can be considered as a genetic reserve, leading in subsequent phenotypic resistance to these drugs. Conclusion: whole genome sequencing revealed a change in the genetic information, which we associate with the long-term impact of technogenic factors.