Anti-parasitic preparation acute insecticidal activity in vitro study
An insecticide is a substance (or mixture of substances) chemical or biological origin, designed to annihilate harmful insects. The main task of the last decades is development and implementation of efficient and less environmentally hazardous preparations. Today's insecticides for pets have made a significant breakthrough, a large number of new flea and tick remedies has been produced. However, what is the main difference of current purchasable flea remedies, is active ingredient, which provides protection for the animal. Acute insecticidal activity, i.e. the ability of the preparation to cause destruction of insects, is one of the important characteristics of insecticides. The study of acute insecticidal activity of the new integrated anti-parasitic preparation was conducted aiming to obtain data for faster selection of doses in the subsequent conducting of experiments. In laboratory studies, rat fleas Xenopsylla cheopis Roth. insectarium culture has been used by us. MAC50, MAC95(mean-annihilation concentration, providing insect death rate of 50% and 95% in the experiment, respectively) indices were defined by L.C. Miller, M.L. Taitner method. MAC50 has formed 0.000071%; MAC95 has formed 0.003%. Insecticidal activity was also assessed by the destruction of fleas in the experiments compared to blank. High insecticidal activity of the new anti-parasitic preparation in respect of fleas is observed at a concentration of 0.1% and 0.01%. The results of the study of insecticidal activity of this remedy are evidence of its high acute insecticidal activity in respect of fleas.