Expression of gene p53 and matrix metalloproteinase-9 in different histological types of uterine sarcoma
Relevance. Today there is no doubt that the prognosis for female genital malignancies is based on early diagnostics and timely start of treatment. Such prognosis shall include the assessment of invasive and metastatic tumour potential to allow future individualization of antiblastome treatment. The ability of tumour cells for invasive growth and metastatic spread depends on the ability of the matrix metalloproteinase system to split the components of extracellular matrix. It is especially important in sarcomas that have a high frequency of relapse. Purpose of the study: To determine the expression of p53 gene and MMP-9 in different histological types of uterine sarcoma in order to reveal possible interlations. Results: 38.9% patients with overexpression of mp53 gene had a relapse. 4 (33.3%) out of 40.0% patients with negative expression of p53 had a relapse. The maximum frequency of relapses was observed at low expression of p53 - mean value 0.0-6.0%. The expression of mMp-9 was detected in 58.6% cases. The high level of MMP-9 expression was noted in 63.6% cases of leiomyosarcoma, the middle and low levels - in endometrial stromal sarcoma (28.6 and 42.9%, respectively), and no expression - in undifferentiated sarcoma (72.7% cases). Conclusion: Co-expression of the mutated p53 gene and MMP-9 established in tumour tissue indicates interlation of their transcriptional activity and can be indicative of prognostic significance of the studied molecular genetic markers. The combination of overexpression mp53 even with a moderate expression of MMP-9 corresponds to the most unfavorable course of the process observed in undifferentiated uterine sarcoma. A high level of MMP-9 expression in the absence of mp53 gene expression corresponds to a more favorable prognosis for leiomyosarcoma. In case of endometrial stromal sarcoma, the overexpression of mp53 is observed at extremely low expression of MMP-9 and is also characterized by a more favorable prognosis.