Obtaining of the Complex Enzyme Preparation with Enhanced Pectinase Activity Using a New Mutant Strain <i>T. reesei</i> Co-44
In order to obtain a complex enzyme preparation providing efficient hydrolysis of the main plant non-starch polysaccharides — cellulose, xylans, and pectin, studies were conducted to optimize a fermentation medium composition for submerged cultivation of a new mutant strain <i>T. reesei</i> Co-44 – a highly active producer of endo-carbohydrases. A concentrate of soy low molecular components was chosen as the inducer of the key carbohydrases biosynthesis by the strain. The concentrate provided the maximum increase in polygalacturonase activity – more than 8 times, and an increased level of endoglucanase and xylanase biosynthesis. After cultivation of <i>T. reesei</i> Co-44 under optimized conditions, a complex enzyme preparation Xylorizin K4 was obtained, its physicochemical properties were studied. Using electrophoresis and MALDI⎯TOF mass spectrometry, the presence of endopolygalacturonase (GH28) <i>T.reesei</i> in Xylorizin K4 was confirmed<i>.</i> The studies have shown the potential of Xylorizin K4 application in production of soy protein concentrates to eliminate the main soybean non-starch polysaccharides.