BLOOD PLASMA PROTEINS AND FREE RADICAL ACTIVITY OF NEOPLASM TUMORS OF EPITHELIAL TISSUES
Analysis of relationships between blood proteins content and free radical activity in blood plasma and tumor presence and stage was studied. Blood plasma samples of 39 patients with tumors of epithelial tissues previously non-exposed to anti-tumor treatment and 14 healthy patients without tumor (control sample) have been taken for analysis. Free radical activity was evaluated by induced biochemiluminescence and oxidative modification of proteins was estimated by the level of carbonyl derivatives. The content of blood plasma proteins and their fractions was determined by bioanalyzer. Blood investigation of patients with neoplasms revealed decrease in albumins and β-globulins, while the total plasma protein was not practically changed. Blood analysis in malignant tumors of epithelial tissues showed a significant increase in free radical activity and the degree of plasma proteins oxidative modification. In the initial stages of carcinogenesis processes of both fragmentation and aggregation of proteins predominate (growth of aldehyde and ketone carbonyl derivatives of dinitrophenylhydrazones). Protein aggregation dominates at the fourth stage of carcinogenesis (increase of aliphatic ketone-dinitrophenylhydrazones). Parameters of oxidative modification of blood plasma proteins can be used for preoperative diagnosis of the stage of malignant neoplasms of epithelial tissues.