PERSPEKTIVEN DER ANTIZYTOKINOTHERAPIEGewinnung des RNSAptamers zum humanemInterleukin 6 (hIL6) durch die SELEX Methode
SELEX approach was used to select aptamers to human interleukin6 (hIL6) in a buffer solution mimicking blood saline composition using an RNA combinatory library with a 30nucleotide random region at complexity of 1015 variants. The selection procedure included immobilization of hIL6 on BrCNactivated sepharose by affinity chromatography with hIL6 preserving native conformation of BE8 monoclonal antibody binding site as demonstrated by luminescent microscopy. Fourteen selection cycles resulted in isolation of an RNA aptamer specifically binding hIL6. The aptamer primary structure was determined and a presumptive secondary struc ture was developed by Zuker's algorithm. ArhIL6 specifically bound hIL6 with an appar ent dissociation constant 316±130 nM.