STUDY OF ANTIULCER ACTIVITY OF GEL FORMULATIONS CONTAINING CHITOSAN AND CHITOSAN WITH TAURINE ON ETHANOL AND STRESS GASTROPATHY MODELS IN PRECLINICAL STUDIES
The purpose of the study. Comparative study of the gastroprotective activity of gels containing chitosan 1% and chitosan 1% with taurine 4% in models of stress - and ethanol - induced ulcers in preclinical studies. Materials and methods. Preclinical studies were carried out on 56 white outbred conventional male rats weighing 210 - 250 g. In ethanol model, gastric ulcers were induced by oral administration of 96% ethanol in a volume of 5 ml/kg. In the stress model, stomach ulcers were induced by immobilization in a dorsal position while isolating from extraneous noise and using an air temperature of 5,0±2,0°C in a refrigerator for 4 hours. The studied drugs were administered 1 hour before the administration of ethanol or before the immobilization process. The studies were carried out in compliance with international recommendations on the humane treatment of animals used for experimental purposes, approved by the Ethical Committee for the Expertise of Biomedical Research of the Federal State Budgetary Educational Institution of Higher Education of Voronezh State University, protocol №42 - 04 of 10.12.2018. Results and its discussion. In the preclinical study, the antiulcer activity of gels containing chitosan 1% and chitosan 1% with taurine 4% was studied in laboratory animals using models of stress - and ethanol - induced ulcers at a dose of 0.16 ml/100 g of body weight. Conclusions. An oral gel containing chitosan, with a single prophylactic oral administration, does not show sufficient antiulcer activity in the ethanol - induced ulcer model, but in the stress - induced ulcer model at a dose of 0.16 ml/100 g exhibits enough antiulcer activity (the calculated value of the antiulcer activity index is 2,03), which is significantly lower than the value of the reference drug omeprazole at a dose of 20 mg/kg. The addition of taurine to the gel does not lead to an increase in antiulcer activity.