EXPERIMENTAL MORPHOLOGIC ANALYSIS OF CORNEAL FLAPS IN PENETRATING KERATOPLASTY FORMED WITH FEMTOSECOND LASER
Comparative morphologic analysis of corneal flaps formed with femtosecond laser and mechanic trepan was carried out. The object of observational studies of Fs-rays effect and a mechanical trepanation was a cornea of isolated pig eyes got from slaughter beast (10 eyes) and cadaver donor eyes inapplicable for transplantation [5]. A femtosecond laser «IntraLase Fs» with pulse frequency 60 khz, energy per pulse 2,3–2,5 micron J, impulse spacing 2–3 mkm, levels spacing 2–3 mkm, and metal plunger trepans were used to form corneal disks. It was taken a histological analysis of cornea probes submitted for Fs-laser and mechanical manipulations. It was revealed that impact of femtosecond laser on corneal tissue is less harmful, absence of corneal stromal fibers’ deformation in cut edge and its central parts. Femtosecond laser provides precise cut of corneal flap.