BIONFORMATICAL ANALYSIS OF THE LOW-MOLECULAR-WEIGHT FRACTION OF <i>PENICILLIUM DIGITATUM</i> PROTEOME
The annotation of the mass-spectrum, which was obtained by MALDI-TOF-mass-spectrometry of the mycelium extract of Penicillium digitatum 1 using a protocol suitable for species identification, was carried out in this study. Proteins and peptides which most likely forming the mass-spectrum were identified with using a series of bioinformatics resources (TagIdent, Protein BLAST, Swiss-Model and RaptorX), as well as it was possible to reconstruct their tertiary structure, to reveal homologs among other species of micromycetes, to establish some other properties based on amino acids sequences. As a result of this work, 33 polypeptide of the low-molecular-weight fraction of proteome were detected and described, of which 9 - have homologs in other micromycetes and 13 - are unique for the studied species. Most of the investigated polypeptides were previously predicted only by molecular genetics methods and are not provided with detailed structural and functional characteristics.