Molecular genetic analysis of genome of Glubinnoe/2004 high virulent strain of tick-bone encephalitis
The authors present molecular genetic analysis of sequencing of genome of Glubinnoe/2004 high virulent strain of tickbone encephalitis (Far Eastern sub-type). Upon comparing this genome with the genomes of other strains of the Far Eastern, Siberian, European sub-types, the authors indicate that the Glubinnoy/2004 strain has a number of mutations in the unstructured virus proteins, virus polyprotein processing sites and promoter sequences of the genome. These mutations are likely to cause accelerated replication and maturation of the virus. As shown, the secondary structure of promotor 5end genome domain and the position of transmembrane domains in NS2A protein distinguish the FE-subtype strains from the Siberian and European subtypes of the tick-bone encephalitis.