Procedure of sterilisation and storage of histamine solutions with low concentrations
Background: study of the biological activity of histaminesolutions after sterilization by autoclaving and membranefiltration as well as the assessment of the impact of single andrepeated freezing and defrosting on the stability of preparedsolutions. Material and methods. Histamine dihydrochloride solutionat a concentration of 10 μg//ml was sterilized using membranefilters or autoclaved at 121 °C for 30 minutes, some of thesamples were frozen in sterile insulin syringes, followed by1- and 5-fold defrosting. Skin prick tests have been performedon one or both hands with the resulting solutions. Results. Comparison of the results of skin prick tests performedwith histamine solutions sterilized in various ways showed nostatistically significant differences (p>0,05). No statisticallysignificant differences between the results of trials performedwith histamine solutions that hadn't been frozen before, after1- and 5-fold freezing and defrosting were found (p>0,05).When comparing the results of skin prick tests performed nostatistically significant differences were found (p>0.05).Conclusion. It has been shown that autoclaving at 121°С for30 minutes followed by freezing and defrosting of histaminedihydrochloride solutions does not lead to a significant changein their activity.