FEATURES OF SERUM INFLUENCE ON MOUSE OOCYTE MEIOTIC MATURATION IN VITRO
The effects of serum addition to SOF medium particularly or with Pyruvic acid or lactate on mouse oocyte meiotic maturation in vitro, were studied. Addition of serum promoted maturation to metaphase 2 80.84±3.83 % oocytes with cumulus but 61.64±10.20 % denuded oocytes remained on diplothene stage. Supplemental addition of lactate increased the meiosis initiation in denuded oocytes but almost didn’t effect on development of oocytes in cumulus. Addition to medium both serum and pyruvate promoted to meiotic maturation of 66.63±18.00 % oocytes with cumulus and 47.94±6.03 % denuded oocytes, that almost didn’t differ from variant with only pyruvate addition. For all variants serum addition to medium increased the growth and expansion of cumulus but in supplemental addition of lactate degraded the ease of cumulus separation. Serum addition increased the quantity of oocytes with degenerated chromosomes, that indicates on requirement for additional study of expediency of serum use in system of embryos production in vitro.