ANTIGENE EXPRESSION KINETICS FOR HUMAN CML PHCELLS UNDER CELL PROLIFERATION AND DIFFERENTIATION IN THE SUSPENSION CULTURE
Expression kinetics of 8 antigens (CD34, CD33, CD15, CD13, CD11b, CD14, CD3, CD19) under cultivation of Ph<sup>+</sup>,BCR/ABL<sup>+</sup>cells that are resulted in chromosome translocation t(9,22) and determined CML arising and progression have been studied in comparison with cell differentiation kinetics tested by cell morphology. Under 14 days cultivation in vitro of Ph<sup>+</sup>,BCR7ABL<sup>+</sup> mononuclears from periferal blood of CML patient with b3a2 <i>BCR/ABL </i>mRNA there are identified additionally to cell differentiation according to CML myelopoiesis scheme also the active CD34 antigen expression on granulocytes and lymphocytes precursors. Activ CD34<sup>+</sup>cell accumulation occurs at cultivation finish under significant decrease of Ph+mononuclear concentration due to differentiation and apoptosis. Expression kinetics of CD34 antigen and other antigens look like two-phases pattern with noticeable activation of antigen expression close to cultivation finishing. There is strong correlation between CD34 antigen expression and cell proliferation in the first cultivation phase and especially in the second phase proliferation (7-14 days). CD34<sup>+</sup>cell proliferation that are mainly presented by CD34<sup>+</sup>myeloid precursor cells is assumed to be resulted in exit of G<sub>0</sub>/G<sub>1 </sub>phases of cell cycle with consequent partial differentiation in the cytokine presence and conservation in G<sub>0</sub> without cytokines. Simultaneous CD34 antigen expression on blast cells (myeloid and lymphocyte precursors) in the Ph+mononuclears studied shows that chromosome translocation t(9,22) resulted in early cell precursors of this CML patient -that look like is an polipotent hematopoietic stem cells or hematopoietic stem cell itself.