Perfection of serum diagnostics methods in system of epidemiological supervision for a spotted fever group ricketsioses
The results of approbation and detection of efficiency of indirect enzyme-linked immunosorbent assay (ELISA) for serological diagnostics of the infections caused by spotted fever group rickettsiae in West Siberia have been presented. Application of experimental tests-systems for detection of IgM and IgG antibodies to R.sibirica antigens in twin blood serums has allowed to verify the diagnosis of tick-borne ricketsiosis in patients from Altai region in majority of cases, including negative results of complement fixation test. Using rickettsial antigens of various species-specificity in indirect ELISA opens prospects for large-scale sero-epidemiological assay of geographical distribution of old and new species of rickettsiae and their roles in a human pathology.