Adhesion and viability of human fetal fibroblasts cultured on 3D printed polycaprolactone matrix
Synthetic polymer materials are of great importance to tissue engineering. Such material must provide sufficient strength to tissue engineering construct, be biocompatible, biodegradable, non-immunogenic, while being inexpensive and convenient to use. The polycaprolactone (PCL) - a linear aliphatic synthetic polymer, meets all of these criteria. In the tissue engineering process the most important issue is the interaction of the tissue matrix with cells. In this paper we evaluated the adhesion and viability of human fetal fibroblasts seeded on 3D printed PCL scaffold. According to our results, fibroblasts effectively adhered to the PCL scaffold and were metabolically active. A resazurin test demonstrated a linear increase in fibroblast viability during cultivation on the PCL scaffold, its value increased more than 2 times from the 1st to the 4th day of culture. It was shown that PCL matrix even without additional modifications can be effectively used in tissue engineering.