INTERFERON SYSTEM GENE ACTIVITY IN COLON ADENOCARCINOMA CELLS HCT-116: REGULATION BY INTERFERON-ALPHA-2B FROM BACTERIA OR PLANTS
Broad activity spectrum of IFNs has assured its wide application as antiviral and antibacterial agent. However this cytokine is used for treatment of certain cancer types only. Mainly it is explained by lack of knowledge about IFN antitumor action mechanism. Hereafter we describe the study of IFN system gene activity in colon adenocarcinoma cells (HCT-116) using RT-PCR. Human lung embryo fibroblast (HLEF-977) was used for comparison. Multiple IFN system functional genes were analyzed. Five medicaments, produced in Russia, were compared with each other. Results show that the inherency of cancer HTC-116 cells is absence of expression of several apoptosis signal regulator and IFN system genes. The IFN-alpha family gene activity level is lower, but protooncogene Bcl-2 shows high activity and IFN-ИФН-λ-1, IFN-depended enzymes, IL4 and IL17 gene expression levels are higher in these cells. Positive feature of analyzed medicaments is suppressed protooncogene Bcl-2 expression and activated IFN- α, IFN- λ-1 and IFN-depended enzymes gene transcription colon adenocarcinoma cells HCT-116.