CONSTRUCTION OF THE RECOMBINANT CHIMERIC POLYPEPTIDES BASED ON BMPA, P83 AND OSPC PROTEINS OF SIBERIAN ISOLATES OF BORRELIA GARINII AND BORRELIA AFZELII AND COMPARATIVE STUDY OF THEIR IMMUNOCHEMICAL PROPERTIES
Gene regions encoding mature BmpA, p83, OspC proteins were amplified by PCR, inserted into pETm expression vector and cloned into E. coli Rosetta 2 cells. E. coli strains - producers of the chimeric polypeptide BmpA-p83, consisting of the epitopes of BmpA and p83 mature proteins from B. garinii, as well as a chimeric polypeptide OspCg-OspCa, consisting of the epitopes of mature OspC protein from B. garinii and of fragment OspC from B. afzelii were obtained. It was demonstrated that chimeric polypeptides specifically bind anti-bodies from serum of patients with Lyme disease. These results show the availability of obtained chimeras for the serodiagnosis of Lyme disease.