INFLUENCE OF POLYAMINS ON PROLIFERATION AND DIFFERENTIATION OF HONDROCYTES OF GROWTH PLATES OF RING VINES IN RITS
Proliferation and differentiation of growth plate chondrocyte (VBGP) during growth period plays a key role in the regulation of the development of the vertebral growth plate. Numerous data suggest that the kinetics of growth of indices of the growth plate of vertebral bodies is higher in patients with adolescent idiopathic scoliosis (AIS). In the pathogenesis of AIS, the hypothesis of the effect of melatonin synthesis dysfunction is actively discussed. Nevertheless, the mechanism of regulation of proliferation and differentiation of chondrocytes of VBGP in AIS patients is largely unknown. On the other hand, it is known that the level of polyamines is an indicator of the regulation of cell growth, proliferation, tissue regeneration and malignancy. The purpose of this study was to compare the regulatory role of malathonin and polyamines on the proliferation of RPTC chondrocytes. The object of the study was the culture of chondrocytes, obtained from the growth plate of the vertebral bodies of rats. Cell proliferation assays were performed using the standard MTT test. The inhibitory role of melatonin on the proliferation of chondrocytes of the growth plate of the vertebral bodies of rats in a test with 3- [4,5-dimethylthiazolyl-2-el] -2,5-diphenyltetrazolium bromide (MTT test) is shown. The potentiating effect of spermine on the inhibitory effect of melatonin and the activating action of spermidine on the proliferation of chondrocytes in the growth plate of the vertebral bodies of rats is shown for the first time.