ESTIMATION OF THE METHOD OF EXTRACTION THE CILIATED EPITHELIUM OF THE NASAL SEPTUM IN LABORATORY RATS
In the mucous membrane of the upper respiratory tract, under the influence of constant trigger factors, an inflammatory reaction occurs, leading to the formation of mucociliary insufficiency, which plays a significant role in the formation of chronic inflammation. In this regard, the need for an in-depth study of the cellular bases of the ciliated epithelium is actual topic. With the introduction of modern methods of surgical and topical action on the mucous membrane of the nasal cavity and paranasal sinuses in otorhinolaryngology to assess their effect on the mucociliary system, the development of experimental models of diseases of the ENT organs in various laboratory animals does not lose its relevance. In laboratory rats, the most accessible place for mucosal sampling is the nasal septum. The development of a method of its extraction remains relevant, the main task of which will be to preserve its anatomical integrity. Objectives - estimation of the quality of the developed step-by-step technique of extraction of the ciliated epithelium of the nasal septum in laboratory rats. Materials and methods. The technique of extraction was tested on 150 male rats. In the course of the study, the parameters characterizing the quality of the proposed sampling technique were analyzed by assessing the anatomical preservation of the obtained macro- and micropreparations. Results. The technique of extraction the ciliated epithelium of the mucous membrane of the nasal septum from laboratory rats is described step by step. The integrity of the cartilage and mucous membrane of the nasal septum was preserved in 134 (96,4%) preparations, visualization of all epithelial structures was noted in 130 (93,5%) preparations, visualization of morphofunctional zones in 135 (97,1%) preparations. The number of micropreparations that met the criteria that determine the quality of the developed technique amounted to 121 (87,1%) preparations. Conclusion. Necropsy of laboratory animals with subsequent histopathological examination of organs and tissues are the main methods of studying and evaluating the results of the experiment. The proposed method of sampling the ciliated epithelium of the mucous membrane of the nasal septum in laboratory rats, while observing the sequence of all stages, makes it possible to obtain high-quality micropreparations with preserved anatomical integrity of all structures, which is important for obtaining reliable results of pathomorphological research.