Effect of chemical composition and surface roughness of TiNi on proliferation properties of mesenchymal stem cells
Investigation results of influence on proliferation of the rat mesenchymal stem cells and an estimation of cytotoxicity of surfaces of the titanium nickel samples, prepared by special mechanical and electrochemical methods and differing with morphology and the roughness are presented in the article. It is shown that the studied samples do not render toxic action on the rat mesenchymal stem cells. The rat mesenchymal stem cells kept viability, adhesive, morphological properties and ability to proliferation in vitro at their cultivation in the presence of the tested metal samples or on the samples surface. These results are confirmed by the following methods - cell counting in the Gorjaev chamber, by the MTT-method, flow cytometry, optical and fluorescent microscopy. It is established that the proliferation processes of the rat mesenchymal stem cells are expressed poorly when the morphology and a roughness correspond to the class С10-11 as it observed on the surface of samples ТН1(B) prepared by multistage mechanical grinding to mirror shine and then electrolytically polished. On the contrary, the morphology and a roughness of the class С7 on the surface of samples ТН1(A) reached by the chemical etching and the following electrolytical polishing are more optimum for the rat mesenchymal stem cells proliferation.