The use of bone marrow stem cells and nanoparticles of perfluorocarbon emulsions for precise drug delivery
Data obtained in this work denotes the possibility of a loadinged pharmaceutical composition (PC) using nanoparticles of perfluorocarbon (PFC) emulsion to stem cells (SCs) used for subsequent PC delivery to different organ tissues. The quantity of PC adsorbed in the emulsion particles and injected into SCs depends on the chemical nature of the surface-active substance used to stabilize the emulsions. Using on the example of chlorine e6 and doxyrubicin, it was shown that PC can be adsorbed in the PFC emulsions particles forming a «nanoparticle-PC» complex which can be injected into stem cells and delivered to the regions of with enhanced proliferative activity. SCs charged with the complex are capable of keeping their morphological characteristics for a long period of time (up to 15 days have been observed of observation) as well as their migration function within the recipient organism (up to 4-5 days have been observed of observation). Experiments on animals with spontaneous breast tumour have shown that after the presence, following transplantation, a donor’s of donor SCs containing nanoparticle-chlorine e6 complex were detected in the tumour tissues and in target organs. In preliminary experiments with using the chromato-mass spectrometry approach, it was shown that tumour tissues accumulate up to 14% of injected donor SCs, and up to 14% of PC. Treatment of donor SCs extracted from recipient organisms in 4-5 days after transplantation with laser irradiation led to their death, indicating the presence of chlorine e6, which preserves its medical features for this period of time.