Effect of an antibiotic on gut microbiota and activity of digestive and antioxidant enzymes of <i>Galleria mellonella</i>
The widespread use of antibiotic substances leads to the occurrence in the environment of a large number of antibiotic-resistant microorganisms, the antibiotics themselves and their decay products. This is accompanied by the formation of bacteria multi-resistant forms in the environment, including in the gut of various animals, and their successful adaptation in various environmental conditions. The antibiotic (amikacin) effect on the larval midgut microbiota, as well as the activity levels of antioxidant and digestive enzymes, was studied over ten generations of the great wax moth Galleria mellonella . In addition, there was a twofold increase in the digestive enzymes activity and the restoration of the antioxidant enzymes activity to values in the control group in the tenth generation. The changes which we registered in the digestive enzymes activity and antioxidant system enzymes can be considered as an adaptive mechanism of the antibiotic substances effect on both the host organism cells and the microbiota.