DETERMINATION OF THERMODYNAMIC BINDING PARAMETERS AND TYPE OF INTERACTION BETWEEN THE INFLUENZA VIRUS HEMAGGLUTININ AND PHOSPHATIDYLCHOLINE LIPOSOMES
At the moment, the antiviral activity of polyallylamine polyelectrolyte with a molecular weight of 8000 (PAA) against herpes simplex virus type 1 and cytomegalovirus has been reliably proven. The non-toxic range of PAA concentrations was determined while maintaining antiviral activity. However, information regarding the metabolism of this compound has not been obtained. In this work, we studied the content of cytochrome P-450 during the metabolism of PAA, which has antiviral activity, using electron paramagnetic resonance (EPR) and direct determination of the content of cytochrome in microsomes of mouse liver cells. A decrease in the amplitude of the EPR signal of the low-spin form of cytochrome P-450 with a g-factor equal to 2.25 was revealed at D2= 2970 mg/kg, at D1 = 990 mg/kg the signal amplitude was the same as in the control. At D2= 2970 mg/kg, there was also an increase in the EPR signal of dinitrosyl complexes of non-heme iron with sulfur-containing proteins with a g factor of 2.03, which is associated with a violation of the integrity of liver cell membranes. Based on the data obtained, it can be concluded that the optimal dose for the metabolism of PAA by the liver microsomal oxidation system is D1 = 990 mg/kg. When directly measuring the relative content of cytochrome P-450 in liver microsomes at the initial time and after 60 min, a pronounced decrease in its concentration was found relative to the initial one at D2 = 2970 mg/kg, at D1 = 990 mg/kg, only a slight decrease in the content was observed cytochrome compared with the initial.