The fixed human lymphocytes as a source of DNA for polymerase chain reaction diagnostic
The article presents the results of studying of quantitative and qualitative characteristics of DNA samples separated by sorbate techniquefrom human lymphocytes went through ethanol acetic fixation for preparation ofcytogenetic specimen. The concentration of DNA varied within range 18.9-213.2 ng per mkl. The rate A260/A280 amounted to 1.47-2.53. All samples were found suitable for application ofpolymerase chain reaction during detection of AZF-deletions of Y-chromosome. The considered mode of separation of DNA is highly efficient in respect of fixed lymphocytes and it can be applied in medical biologic studies.