Spectroscopic determination of binding Human Serum Albumin with dimethyl sulfoxide-platinum (II) complex
<i>Binding of cis-[Pt(Me</i><sub><i>2</i></sub><i>SO)</i><sub><i>2</i></sub><i>Cl</i><sub><i>2</i></sub><i>] to human serum albumin (HSA) and the effect of the complex formation on the structure of protein have been studied by electronic absorption and fluorescent spectroscopy methods. The binding constant of cis-[Pt(Me</i><sub><i>2</i></sub><i>SO)</i><sub><i>2</i></sub><i>Cl</i><sub><i>2</i></sub><i>] to HSA (K</i><sub><i>B</i></sub><i> = (1.2 ± 0.1) · 10</i><sup><i>3</i></sup><i> M</i><sup><i>-1</i></sup><i>) and Hill's coefficient (h = 1.03 ± 0.1) have been determined. It has been found that the binding of cis-[Pt(Me</i><sub><i>2</i></sub><i>SO)</i><sub><i>2</i></sub><i>Cl</i><sub><i>2</i></sub><i>] to a protein causes the internal packing of the protein molecule to change.</i>