IMPACT OF MATERNAL AND NEONATAL FACTORS ON CORD BLOOD SAMPLE QUALITY
Objective. To estimate the influence of the mother age and the neonatal factors such as the birth order, the birth weight, the gestational age, and the baby sex on the quality of the UCB unit in Belarusian population. Materials and methods. 1213 UCB units from the personal storage bank of the PC of the Center for Pediatric Oncology, Hematology and Immunology of the Republic of Belarus were analyzed. The effects of maternal characteristics including the maternal age and the delivery method, the birth order and the neonatal factors such as the birth weight, the gestation duration, and sex on UCB quality were determined basing on the collected blood volume, the total nucleated cell (TNC), CD34+ cell, colony forming units-granulocyte macrophage (CPU-GM), and burst forming unit-erythroid (BPU-E) counts. Results. In the univariate analysis, bigger babies were shown to have statistically higher TNC counts, more CD34+cells, more CPU-GM and BPU-E. CB volume when collected in a cesarean section. The TNC counts was statistically higher in a normal vaginal delivery, in the first-borns, the female babies, and the gestational age older 39 weeks. A higher CPU-GM count was associated with the first baby and the gestational age more than 39 weeks. The maternal age showed no effect on the laboratory parameters that were assessed. Our research showed that optimal CB units for cryopreservation in the public CB bank could be obtained by collecting CB from infants with body weight more than 3500g. In addition, it was recommended to collect CB unit in a volume more 40 ml as the odds ratio would increase obtaining samples of a high relevance by 49%.