Design, synthesis and use of synthetic oligonucleotides
Synthetic oligonucleotides are widely used in modern molecular biology, genetic engineering, biotechnology and medicine. They are essential components of wide range methods of analysis of genes and genomes, such as PCR, sequencing, microarray and other. Design, construction, and the synthesis of DNA primers and oligonucleotide probes bearing a variety of reporter molecules is an independent scientific and practical task for solutions which there are sophisticated and high-tech equipment, software, and special working protocols. This review provides a brief description of methods of design, synthesis and use of synthetic oligonucleotides.