Selective Inhibitor of Histone Deacetylase 6 - Tubastatin A - Supresses Proliferation of Hepatitis C Virus Replicon in Culture of Human Hepatocytes (accelerated publication)
Acetylation of а-tubulin was studied in cultures of human hepatocytes under the action of selective inhibitors of his-tone deacetylases HDAC6 and Sirt-2 - tubastatin A and 2-(3-phenethoxyphenylamino)benzamide, respectively. It was found that acetylated а-tubulin accumulated in hepatocytes cell line HepG2 predominantly when HDAC6 but not SIRT-2 was inhibited. Under the same conditions, а-tubulin acetylation in hepatocytes of cell line Huh7 was not observed at all. However, inhibition of HDAC6 led to а-tubulin hyperacetylation and simultaneously to decrease in viral RNA concentration in hepatocyte Huh7-luc/neo cell line that supported propagation of the full genome repli-con of hepatitis C virus. The correlation between these two processes points to HDAC6 as perspective cellular target for hepatitis C therapy.