Study of miRNAs in patients with hypertrophic obstructive cardiomyopathy
Objective. This study was sought to explore the expression and role of miRNA in patients with hypertrophic obstructive cardiomyopathy. Material and methods. miRNA were quantified by real-time polymerase chain reactions in 16 patients with hypertrophic obstructive cardiomyopathy and 2 health donors (mean age 38,1±13,9). Endomyocardial biopsies were obtained from the intraventricular septum during the surgical myectomy. Results. In patients with HOCM myocardium expression of anti-hypertrophic mir-1, mir-133a and mir-133b was increased, expression of pro-hypertrophic mir208b and mir-214 was significantly higher and expression of pro-hypertrophic mir-208a and mir-195 was decreased in comparison to normal myocardium. We used RNU19 as a control gene. Conclusion. Our results may become a foundation for the identification of new targets for influence by means of antagomirs for prevention of heart hypertrophy and decrease of progressive course of the disease.