Determination of polysaccharides and free sugars sum by UV-spectrofotometry in Tussilago farfara leaf
The procedure of polysaccharides and free sugars sum determination with phenol's reagent (after the acid hydrolysis) by UV-spectrofotometry in the water extraction from the medicinal plant raw materials (Tussilago farfara leaf) is offered; the main metrological characteristics are estimated. The developed procedure is validated by standard addition method. The procedure has satisfactory metrological characteristics. The relative measurement error doesn't exceed 5%.