INTEGRATED ACTION OF POLYMERIC GUANIDINE DERIVATIVESON CELL CULTURES
The article presents results of the study of polyhexamethylene guanidine (PHMG) on cell culture of bovine trachea and chicken embryo fibroblasts. It is found that PHMGchloride in 10-4 % concentrations or high has a biocidal effect on the formed monolayer of cells, in 10-5 % (0,1 mg/ L) concentration and lower - almost not affected on it. At 10- 5-10-6 % concentrations PHMG chloride completely suspends the proliferative activity of chicken embryo fibroblasts. From 0,3×10,7% to 0,3×10,9% drug concentrations are partially inhibited mitosis of fibroblasts and retarded the process of the monolayer formation. In short-term 15-minute pre-exposure it is contrary - PHMG salt in 10-9 -10- 7 % concentration range can stimulate cell proliferative activity. The 10-7 % concentration of PHMG chloride and the 10-8 % of PHMG succinate show the best stimulating activity for trachea cells.It was revealed first that the short-term exposure PHMG protects already formed monolayer of cells from virus. Herewith virus adsorption on the cell surface and its penetration inside are blocking. Virus protective effect of PHMG salt depends on the anionic composition, duration of exposure, concentration of cells and virus. There were tested viruses, which contain lipids in its shell. Namely herpesviruses equine rhinopneumonitis and bovine rhinotracheitis, as well as the retrovirus of equine infectious anemia. It is determined that 10-3 -10-2 % concentrations of PHMG chloride at 15 minutes, and 10-2 % concentration of PHMG succinate at 30 minutes exposure provide reliable protection of cells monolayer from damage by viruses. In addition, for transformed continuous cell culture of calf trachea PHMG concentrations should be much higher than those for primary fibroblast cultures.