COMPARISON OF NUCLEOTIDE SEQUENCE OF THE GENE <i>VVDXS</i> FRAGMENT IN MUSKAT GRAPES VARIETIES
In the breeding of grapes, the presence of muscat aroma in the berries is a valuable feature. The Muscat grapes varieties have different shades of aroma: an ordinary aroma, spicy, the smell of an orange flower, the shade of tea rose and others. The origin of grapes varieties with muscat flavor has not yet been established. The absence of muscat forms among wild grapevines and the dominance of this feature in hybrid progeny allow us to suggest that this sign is originated in the culture. Muscat aroma is characterized by the accumulation of monoterpenoids in the berries. Monoterpenoids are found in significant concentrations in the grapes of Muscat group and in moderate ones in some other aromatic varieties. A number of studies have shown that the accumulation of monoterpenoids in a grape berry is associated with the gene VvDXS. In the present paper, we investigate the fragment of the sequence of the gene VvDXS in the grapes varieties with muscatel flavor to study the possibility of creation of DNA marker for breeding. The main method used in the study are the polymerase chain reaction (PCR) and sequencing of reaction products by using an automated genetic analyzer ABI Prism 3130. Muscat varieties Novoukrainskiy Ranniy, Frumoasa Albe, Barkhatnyi, Astanikskiy, Irshai Oliver, Litdar, Muscat Gamburgskiy, Alina, Larni Muskatnaya and the variety without muskat aroma were studied. Polymorphism in the sequence of the studied fragment of the VvDXS gene in the studied genotypes have been identified, however, the correlation linked with the studied sign "muscat flavor" is not revealed.