PRODUCTION OF REACTIVE OXYGEN SPECIES BY MONOCYTE-DERIVED MACROPHAGES FROM THE BLOOD OF HEALTHY DONORS AND PATIENTS WITH IHD
Production of reactive oxygen species (ROS) by macrophages from blood monocytes of healthy donors (MP<sub>N</sub>) and patients with IHD (MP<sub>IHD</sub>) before, during, and after their incubation with low-density lipoprotein (LDL) isolated from the blood plasma of healthy donors (LDL<sub>N</sub>) and patients with a high cholesterol level (LDL<sub>H</sub>) was estimated by the method of luminol-dependent and stimulated by opsonized zymosan (OZ) or phorbol-12-myristate-13-acetate (PMA) chemiluminescence (CL). Intrinsic luminol-dependent, and zymosan - or PMA-stimulated chemiluminescence of MP<sub>IHD</sub> have exceeded the same types of chemiluminescence of MP<sub>N</sub> by factors of 1.4, 1.8, 27, and 1.6, respectively (p<0.05-001) The effect of zymosan on MP<sub>N</sub> and MP<sub>IHD</sub> was stronger than that of TPA by factors of 4.3 and 3.2, respectively, but manifested itself 25-3.0 times slower LDL<sub>N</sub> and LDL<sub>H</sub> incubated with MP<sub>N</sub> during 15-60 min increased ROS production by a factor of 1.4 and 2.5 respectively, but influenced ROS production by MP<sub>IHD</sub> (as estimated by luminol-dependent chemiluminescence). Effects LDL<sub>N</sub> and LDL<sub>H </sub>on MP<sub>IHD</sub> were not detected at all. Repeated increase in zymosan-stimulated CL of MP<sub>N</sub> was also observed after their 15-180 min preincubation with LDL<sub>N</sub> and LDL<sub>H</sub> which followed after taking out LDL, washing MP<sub>N</sub> and adding Hanks' solution with opsonized zymosan. This increase was also stronger after MP<sub>N</sub> incubation with LDL<sub>H</sub> than after MP<sub>N</sub> incubation with LDL<sub>N</sub>, and no increase was observed in experiments with MP<sub>IHD</sub>. Thus, the results obtained by a chemiluminescent method showed that fresh macrophages from the blood of patients with IHD had higher ROS production than macrophages from healthy donors. LDL<sub>N</sub> and LDL<sub>H</sub> could exhibit primary and secondary (after preincubation) stimulating effect on CL in MP<sub>N</sub>; but had no effect on MP<sub>IHD</sub> An analysis of macrophage chemiluminescence is a sensitive test for evaluation the degree of macrophage's stimulation and it may be effectively used for the dynamic control for treatment effectiveness in clinics