Izolation and Characteristics of a Bacillus subtilis K-1-1 Polypeptide, an Inhibitor of Phytopathogenous Fungi and Bacteria Growth
The antibiotic activity of the Bacillus subtillis К-1-1 extracellular metabolites has been investigated. An antimicrobial polypeptide has been purified by gel filtration on ultragel AcA44 and chromatography on Mono Q column. The molecular mass of the polypeptide determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate is 13 kDa. The antimicrobial effect of the polypeptide on fungi Sclerotinia sclerotiorum, Phomopsis helianti, Botrytis cinerea, Fusarium oxysporum, Fusarium sporotrichiella, Sclerotium bataticola and bacteria Erwinia carotovora, Pseudomonas syringe, Pseudomonas marginata, Pseudomonas aureofaciens has been shown. The biochemical characteristics of the antimicrobial polypeptide are represented. Perspectives of the use of the Bacillus subtillis К-1-1 strain as a biological agent for plant protection against phytopathogenic microorganisms are discussed.