COMPARATIVE ANALYSIS OF THE FREQUENCY DISTRIBUTION OF ALLELES AND GENOTYPES POLYMORPHISMS RS 231775 AND RS 3087243 GENE CTLA-4 IN PATIENTS WITH DIABETES MELLITUS TYPE 1 AND TYPE 1 DIABETES COMBINED WITH AUTOIMMUNE THYROIDITIS
A comparative analysis of the distribution of allele and genotype frequencies of polymorphisms rs 231775 and rs 3087243 CTLA-4 gene in 83 patients with type 1 diabetes mellitus (T1DM) and 91 patients with a combination of type 1 diabetes with autoimmune thyroiditis (AIT). The control group consisted of 108 blood donors without autoimmune diseases, and family history on them. A significant increase in the frequency of allele G (p = 0,005) and genotype GG (p = 0,0116) polymorphism rs 231775 in patients with type 1 diabetes compared to the control. Also found a significant increase in the frequency of allele G (p = 0,0016) of polymorphism in patients with type 1 diabetes in combination with AIT compared to patients with isolated T1D, the results of the trend test set of statistically significant differences in genotype frequencies between the groups (p = 0,002). Significant differences between the allele and genotype SNP rs 3087243 CTLA-4 gene in all groups have been identified. Increasing the frequency of allele G (p = 0,005) and genotype GG (p = 0,0116) in the group of patients with isolated DM1 compared to the control indicates the association of this embodiment, the polymorphic marker gene rs 231775 CTLA-4 with an increased risk of developing type 1 diabetes, coinciding with the data of other researchers.