THE PRODUCTION OF POLYELECTROLYTE ENZYMATIC MICRODIAGNOSTICUM BY NAOTECNOLOGY METHODS AND ITS USING FOR CLINICAL BIOCHEMICAL ANALYSES
A new type of a wide-purpose diagnosticum including a medical use is developed. The major element of the diagnosticum is a multilayer polyelectrolyte nano- and microcapsule with included enzyme, which can provide qualitative and quantitative information on its substrate, inhibitor or activator present in the medium to be analyzed. Compared to enzymatic methods for analysis of biological fluids, available in medicine, the microdiagnosticum we propose has many advantages. An encapsulated enzyme, first, retains its activity for at least several months, whereas the activity of a "free" enzyme in solution falls actually to zero in several days, second, retains the activity in proteinase-containing biological fluids under analysis thus eliminating the need to remove proteinases from the medium, third, it can be used repeatedly thus decreasing consumption of enzyme.