PATHOMORPHOLOGICAL CHANGES IN RAT PANCREATIC TISSUES CAUSED BY LONG ADMINISTRATION OF NIMESULIDE
Aim. The aim of the study was to assess the character of pancreatic tissue lesion in experimental animals when modeling NSAIDs-pancreatopathy using the generally accepted methods. Materials and methods. Modeling of NSAIDs-induced lesion of pancreatic gland in nonlinear white rats was performed by means of per oral introduction of nimesulide (Nise®) during 21 days in the following doses: 0,5 mg/kg (therapeutic dose), 2,5 and 5,0 mg/g. The results were compared with the control group. By the end of experiment, histological material was taken (tissue samples) with observation of ephthanasia rules. Histological methods were used by standard protocols. Results. When using nimesulide (per orally) during 21 days, histopathological changes typical for toxic pancreatitis (vacuolar degeneration, necroses, acinus structure impairment) with marked dose-dependent effect were detected in animal pancreatic parenchyma. In animals with NSAIDs-induced lesion of the pancreas, mast cell were revealed, the number of which visually increased with growth of the dose of this drug.