Special features of <i>Diospyros kaki</i> Thumb. explants introduction <i>in vitro</i>
Establishment techniques of in vitro aseptic culture for Diospyros kaki closed buds during dormancy period and buds while active shoot growth were developed in terms of the research. Concentration of favorable reagents, their treating time and sequence of application were found out as well. The most advantageous terms for yielding of Diospyros kaki starting material were determined in this way. Special features of initial explants` development were revealed while introduction in vitro conditions applying various basic nutrient mediums. Impact of plant growth regulators on regeneration of Diospyros kaki microshoots in nutrient medium was demonstrated in this work.