ISOLATION OF HYDROLYSIS BACTERIA FROM A BIOGASEQUIPMENT WORKING ON CATTLE MANURE AND STUDIIND OF THEIR ENZYMATIC ACTIVITY
This paper presents the results of isolation and screening of hydrolytic microorganisms for biogas production. Efficient biogas production is based on a complex microbiological process, the first stage of which is the hydrolysis of organic substances. From a laboratory single-chamber BU with a working volume of 10 l, using cattle manure as a substrate, and cow manure by inoculation on various elective nutrient media (SPA, MPA, Saburo, MRS), 18 pure isolates were isolated. The cultural and morphological features and viability of isolated microorganisms were studied. Next, screening of microorganisms was carried out according to their hydrolytic activity: amylolytic, proteolytic, cellulolytic, urease, lipase. The maximum GSP index varied within 106-108 CFU/ml. The study of the enzymatic activity of the obtained isolates, namely the activity of hydrolases, showed that 12 isolated cultures have proteolytic activity. Only 2 isolates had amylolytic activity, 11 isolates had the highest cellulolytic activity. 4 isolates had the ability to decompose urea to ammonia. 3 isolates showed lipolytic activity. As a result of the genetic identification of the selected isolates by MALDI-TOF, the isolated microorganisms were assigned to Ochrobactrum tritici, Bacillus pumilus, Pseudomonas citronellolis, Lysinibacillus sphaericus, Acinetobacter johnsonii, Bacillus subtilis, Bacillus cereus, Escherichia coli.