Improvement of cervical cancer screening using determination of microRNA in cytological preparations
The sensitivity of cytological screening of cervical cancer, despite the ongoing technical improvements, remains quite low. It was showed that among women with smears, regarded as questionable cytological data of ASCUS, from 5% to 10%, in fact, they had severe dysplasia. The use of new molecular diagnostic methods can improve the effectiveness of screening of cervical cancer by improving the accuracy of diagnosis of the degree of dysplasia. Neoplastic transformation of the cervix epithelium is accompanied by specific changes in the profile of siRNA expression, the evaluation of which has a great diagnostic potential. In this study, the expression of 18 miRNA was analyzed. The correlation of the expression of five miRNAs (miR-375, miR-20a, miR-196b, miR145 and miR-126) with the degree of neoplasia of the cervix was established, which allowed them to be recommended for further investigation for the purpose of introduction as a diagnostic test at the stage of cytological screening.