The study of immune response of mice immunized intranasally by live influenza cold-adapted vaccine in combination with chitosan derivatives as adjuvants
Different branches of immunity of mice, immunized by live cold-adapted influenza vaccine with chitosan derivatives as adjuvants were investigated. It was shown, that both epidemic A/Krasnodar/101/59 (H2N2) strain and vaccine A/Krasnodar/101/35/59 (H2N2) strain possessed immunosuppressive action and decreased the population CD3, CD3/NK, CD5, and CD4 - positive cells. The inclusion of chitosan derivatives in live cold-adapted influenza vaccine leads to significant proliferation of CD3, CD16, CD4, CD8, CD25, CD71, CD21. Intranasal introduction of live cold-adapted influenza vaccine to mice in combination with chitosan derivatives caused strong activation of TLR-9 and in lesser degree TLR2. In the process of cytokine profile investigation of mice, immunized by cold-adapted live influenza vaccine in combination with chitosan derivatives as adjuvants, we observed faint increase of IL-4 and IL-5 and significant synthesis of IFN-c, that can testify about the development of immune response to Th-1 way. We have made the conclusion that intranasal immunization by cold-adapted live influenza vaccine in combination with adjuvant can produce most balanced variant of immune response.