FUNCTIONAL ACTIVITY OF ARYL HYDROCARBON RECEPTOR IN HUMAN OSTEOSARCOMA CELL CULTURES
Osteosarcoma is the most prevalent bone malignant tumor with a high mortality rate among children and adolescents. The aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor associated with xenobiotic detoxification and carcinogenesis. It is known that some AHR ligands are included in the composition of drugs used in cancer therapy. However there are few works devoted to the study of their effect on osteosarcoma cells. In this work, three primary cell cultures were obtained from biopsy material of malignant bone tumors of human osteosarcoma. AHR protein levels were high in all primary osteosarcoma cell cultures. The functional activity of AHR in osteosarcoma cells was estimated by induction of its target genes by known exogenous ligands: indirubin and indole-3-carbinol. The genes of Cytochrome P450 1 family were analyzed as AHR target genes: CYP1A1, CYP1A2, CYP1B1. It was shown that the aryl-hydrocarbon receptor is functionally active in all l cultures, but the target genes were induced differently by ligand treatment within the same cell culture.