FLUORIMETRIC STUDY OF FILM-FORMING SUBSTANCES CHITOSAN
Chitosancontaning substances are used in medicine, veterinary medicine, food industry, where they have the prospect at the development of a barrier in technologies for processing of livestock products. In accordance with the applications of chitosancontaning substances it is actual the problem of substantiation of technological parameters of processing of chitin-containing raw material. Specific requirements for physico-chemical and functional properties of chitosancontaning substances make actual the means and methods of control of the target parameters, the key of which are qualitative identification and comprehensive determination of chitosan in the composition of film-forming compositions and films coatings. As the chromophore to measure the surface potential of the chitosan substances, we used 1-aniline-8-naphthalenesulfonate (ANS). Although the free dye has rather low intensity of fluorescence, the binding of the dye with chitosan leads to a multiple increase in the quantum yield of fluorescence. There have been correlated parameters of fluorescence spectra of chitosan substances and their physicochemical characteristics (molecular weight, viscosity). It is proved that the maximum fluorescence of the dye in chitosan films is shifted to longer wavelengths compared to chitosan gels, because of the increased polarity of the medium of films on the attitude to gel-like chitosan substances. The data obtained with the use of fluorimetric studies can later be used in the development of methods for the detection of chitosan with the use of ANS as a probe.