Acute toxicity and anti-inflammatory effect study of unripe walnut fruit thick water-alcohol
Topicality. From the literature, it is known that the walnut composition includes tannins, derivatives of ellagic acid, catechins, derivatives of dicarboxylated acids, nucleic acids, amino acids that independently of one another have antioxidant, hypoglycemic, antiproliferative, antimicrobial, anti-inflammatory and many other useful effects in human body. However, no experimental data on the effect of unripe walnut fruit thick water-alcohol extract (ETWA) in the inflammatory process. Aim. To study acute toxicity and anti-inflammatory effect of ETWA. Materials and methods. Study ETWA acute toxicity (LD50) was carried out by the method of Prozorovsky V. K. and co-authors. Studies of ETWA anti-inflammatory effect were carried out on models of carrageenan, formalin and zymosan inflammation of rats’ paw. Results and discussion. In the experiment, ETWA acute toxicity of mice was studied and it was referred to the V toxicity class according to the classification by Sidorov K. K. Based on the results of ETWA anti-inflammatory effect, at a dose of 25 mg/kg, an anti-inflammatory effect was found which is probably based on cell membranes stabilization, inhibition of TNF-α and IL-6 production, inhibition of Pin1 activity, decrease in NO level, and partial suppression of COX and the decrease in enzyme 5-lipoxygenase activity. Conclusions. The received data of the researches allow to consider ETWA in a dose of 25 mg/kg, as a safe anti-inflammatory agent.