THE USE OF ATOMIC FORCE MICROSCOPY IN EARLY DIAGNOSTICS OF NEOPLASTIC PROCESS IN WOMEN
The research purpose was to study the possibilities of using the approaches of atomic force microscopy in the early diagnosis of neoplastic processes in women. Materials and methods. In the experiment, ovarian biopsy material and blood of five women with stage II ovarian cancer aged 45 - 59 years were used. To obtain tumor cells of ovarian tissues, a tissue disintegration technique of 0.025% trypsin was used. In the experiment, the functional properties of ovarian tumor cells and blood cells were analyzed. The functional properties of cell subpopulations (Young's modulus, intercellular adhesion forces) were measured using an INTEGRA atomic force microscope in the power spectroscopy mode. Results. As a result of the study was found a decrease in erythrocyte stiffness by 92.2% (p<0.05) compared with ovarian tumor cells and an increase in the strength of intercellular adhesion in the leukocyte - tumor cell system by 176.6% (p<0.05) compared with the “erythrocyte - tumor cell” system. Conclusion. The obtained data complement the current understanding of the mechanisms of intercellular interaction and they are one of the promising directions in the oncodiagnosis of early ovarian neoplasms.