WORKING OUT AND VALIDATION OF THE METHOD OF QUANTITATIVE DETERMINATION OF FLAVONOIDS IN THE HERB OF LEONTODON AUTUMNALIS L
In the course of the study we have worked out the methodology of differential spectropho- tometric determination of flavonoids in raw Le- ontodon autumnalis L. after the formation of a colored complex with a solution of aluminum chloride. We have established optimal condi- tions for complexation and extraction of fla- vonoids from plant raw materials under study. We have established that the most complete extraction of flavonoids is achieved with fine- ness of 1 mm by extraction with ethyl alcohol of 70% for 45 minutes. The maximum value of the optical density is achieved 30 minutes af- ter addition of 2 ml of a solution of aluminum chloride 2%.