STUDY OF EXTRACTION AND ELECTROPORETIC DETERMINATION OF THEOBROMINE IN WILLOW-HERB
The paper presents the results of the extraction of theobromine from plant materials using the Pluronic block copolymer. The choice of the extractantis substantiated, the method of fermentation and extraction of theobromine is described in detail, and the calculation of the quantitative characteristics of the extraction is given. The regularities of the interfacial distribution of theobromine in the systems willowtea - Pluronic solution, the dependence of the degree of extraction of the alkaloid on the concentration of the block copolymer and temperature have been established. For the quantitative determination of theobromine content in Ivan - tea, the method of capillary electrophoresis is proposed. The choice of conditions and modes of electrophoretic determination of the analyte was carried out, the electrophoregram of Ivan - tea after fermentation is given.