Ultrastructure of Guerin''s carcinoma cells after chemotherapy and local tumor irradiation
Objective: To study ultrastructural pathomorphism of Guerin's carcinoma cells after administration of cisplatin and Taxotere at local fractionated irradiation of the tumor. Material and Methods: Standard electron microscopy was used to investigate Guerin's carcinoma cell ultrastructure after single administration of cisplatin (CP) at a dose of 6 pg/kg and Taxotere (TT) at a dose of 8 pg/kg followed by local fractionated standard x-ray irradiation at a total dose of 10 Gy. Results: It was established that administration of CP resulted in pronounced disorders in Guerin's carcinoma cell ultrastructure and did not influence the number of mitoses in the tumor. Main effect of TT was significant reduction of mitotic activity in the tumor against a background of inconsiderable changes in the cell ultrastructure. Administration of CP followed by irradiation changed little in the structural functional state of Guerin's carcinoma cells while Taxotere administration prior to irradiation caused necroses of the tumor tissue and significant reduction of the number of mitoses in the survived cells. Conclusion: Cisplatin causes pronounced ultrastructure pathomorphism in Guerin's carcinoma cells not influencing the processes of division in the tumor. TT inhibits cell division in the tumor tissue without visible changes in the cell ultrastructure. CP does not possess radiosensitizing properties as to Guerin's carcinoma. TT is an active radiosensitizer considerably potentiating the effect of irradiation on Guerin's carcinoma. Various radiomodifying effects of CP and TT can be associated with their influence on division processes.