MORPHO-FUNCTIONAL RESTORATION OF SIMULATED SEVERE FOCAL BRAIN DAMAGE IN THE BACKGROUND OF TRANSPLANTATION OF MESENCHYMAL STROMAL CELLS IN LABORATORY ANIMALS
In the present study is devoted to the evaluation of the effects of transplantation of bone marrow and adipose tissue MSCs on morpho-functional restoration in the model of brain severe injury in rats. This study was performed on Wistar rats (males and females in equal proportions, with body weight of 250-270 g; n = 166). Traumatic brain injury was produced by original spring-loaded mechanism to precise dosing of impact force. Neurological disorders was assessed by Chen scale, a vertical grid test and a Morris water maze. Evaluation of the functional activity of cells showed that they have similar morphology, phenotype, immunosuppressive and cytokine-secretory activity. In this case, MSC adipose tissue is superior to bone marrow MSC in terms of the number of clonogenic precursors and proliferative activity, as well as in the production of certain cytokines MSC injections on day 1 were accompanied by a significant reduction in neurological deficit Improvement of somato-sensory induced patentsials in the form of amplitude increase and decrease of latency and acceleration of reparative processes in the brain during histological examination in compare with the control group.