Genetic characteristics of reindeer husbandry populations in pedigree farms of Сhukchee Autonomous District
The aim of research is estimation of genetic structure of reindeer husbandry populations in pedigree farms of Chukchee autonomous district. The genetic structure of chukchee reindeer breed has been studied by using ISSR-method on polymorphism DNA microsatellite sequence. It is found 11 amplified fragments of DNA length from 180 to 1300 b.p. In this selection most frequent fragments have middle length - 240 - 570 b.p. In reindeer genomes most frequent fragments are No. 3 (240-330 b.p), No. 5 (350-430), No. 6 (440-520), No. 7 (520-570). It is probably, spectrum of allele frequencies from 4 fragments is typical for chukchee reindeer breed. In this selection all fragments of DNA are polymorphous, their frequencies is less 1. From 11 locus 6-9 (54.5-88.9%) are informative, have frequencies more than 5%. It is established, middle frequent fragments of DNA for chukchee reindeer breed by dates of 7 reindeer husbandry populations. Middle quantity of alleles on locus microsatellite is equaled 9.31 (from 8 to 10.4). Analysis of the data revealed the presence of a high level of heterozygosity DNA - 0.844-0.891. The significance of the data obtained is that they will be used in the formation of the DNA bank for the control and management of the genetic resources of the chukchee reindeer breed.