LIPOSOME STABILITY DEPENDENCE ON FATTY ACID LECITHIN COMPOSITION AND SUNFLOWER PHOSPHOLIPIDS
Phospholipids play an important physiological role being components of biologically active membranes of animals and humans nerve cells. The conditions of separation of sunflower phospholipids have been defined by the method of high performance liquid chromatography. Procedurer for quantitative determination of phosphatidylcholine, phosphatidylethanolamine and phosphatidyl inositol has been developed. It was done under normal phase mode and according to external standards and the order of exit. Phospholipid composition of sunflower lecithin has been identified and phospholipid fatty acid composition has been determined by tandem mass spectrometry. The presence of antioxidant -tocopherol has been determined in liposomes composition, stipulating for maintaining stability of liposomal substance. Membrane-protecting effect of -tocopherol is associated with its participation in arrangement of the membrane structure through direct interaction of its side isodental chain with polyunsaturated fatty acids of phospholipids, which leads to denser packaging of mitochondrial membranes and rising of increased resistance to lipid peroxygenation process effect.