PCR IDENTIFICATION OF PATHOGENIC AGROBACTERIES DETECTED ON VINEYARDS OF KRASNODAR TERRITORY BY THE TYPE OF CONTAINED Ti PLASMIDS
Agrobacterium vitis and some strains of A. tumefaciens and A. rhizogenes cause a dangerous chronic disease on grape plants - crown gall. Using molecular genetic methods, work has been carried out to identify and study the diversity of pathogenic agrobacteria that cause crown gall in the vineyards of the Krasnodar Territory. 76 samples of young and lignified tumor-like outgrowths of crown gall of grapes collected from plants in fourteen vineyards of the Krasnodar Territory and having a different origin of planting material (Italy, France, Austria, Serbia, Bulgaria, Russia) were used as material. In all studied samples, the presence of A. vitis was identified using the PGF/PGR test-system for DNA analysis. When studying the diversity of agrobacteria by the type of Ti plasmids contained therein, four groups were identified: containing octopine-type Ti plasmids (59.5%), octopine/vitopine (13.5%), vitopine (13.5%) and nopaline type (13.5%). The obtained data indicate that in the vineyards of the Krasnodar Territory crown gall of grapes is caused by agrobacteria A. vitis, mainly of the octopine type.