Optimisation of the Method of Obtaining the Monoammonium Salt of Glycyrrhizic Acid from the Roots of the Ural Licorice (<i>Glycyrrhiza uralensis</i> Fisher) of Siberian Populations
We optimised the method of obtaining the monoammonium salt of glycyrrhizic acid (glycyram) from the roots of the Ural licorice (<i>Glycyrrhiza uralensis</i> Fisher) of Siberian populations. The method includes the following stages: extraction of the roots with a 0.5 % solution of NH<sub></sub><sub>4</sub>OH, precipitation of the sum of acids with the help of concentrated H<sub></sub><sub>2</sub>SO<sub></sub><sub>4</sub>, subsequent re-extraction with a 1 % solution of H<sub></sub><sub>2</sub>SO<sub></sub><sub>4</sub> in acetone, salting out the triammonium salt of glyccyrrhizic acid using a 25 % NH<sub></sub><sub>4</sub>OH solution, transformation of this salt into the monoammonium salt (glycyram) by crystallization from glacial СН<sub>3</sub>СООН. Using this method, one may obtain the samples of glycyram with a purity of 85.2-87.5 % (according to the HPLC data). The glycyrrhizic acid content in the investigated samples of licorice roots is 2-4 %.