Application of high-resolution DNA melting analysis in diagnostics of hereditary diseases
Here we have reviewed the molecular basics and capabilities of the high-resolution DNA melting curve analysis for mutation identification. The potential of this technique has been significantly improved by recent developments. The sensitivity and specificity of the high-resolution analysis of DNA melting curves significantly increased because of the emergence of «saturating» DNA dyes and due to the development of instruments for precise measuring the melting changes. HRM analysis (High Resolution Melting) does not require fluorescent probes, does not damage the sample and has high resolving power. Because of its simplicity, specificity, and high sensitivity this method is prevalent in laboratories for DNA scanning and genotyping for the purpose of diagnostics of hereditary diseases.