Improvement of the method of obtaining water-soluble peptides isolated from M. domestica and study of their properties
The article presents data on improvement of the method of obtaining fractions of water-soluble peptides by cold extraction from the biomass of Musca domestica larvae, their separation using dialysis membranes with sizes less than 3.5 kDa, 3.5-7 kDa, 7-14 kDa, more than 14 kDa, followed by a study by means of highly effective liquid chromatography. Retention time and chromatographic yield, protein content, antimicrobial properties, acute toxicity of 2 (BP2) protein fraction, which is the fraction isolated when using membranes of 3.5 - 7 kDa, were established. Therefore, this fraction has a mass of proteins in the range from 3.5 kDa to 7 kDa. The results of the antimicrobial activity of the analyzed protein fraction 2 to the following strains were obtained: B. cereus 8035, E. aerogenes ATCC-13048, E. coli Mordovia 21. It was found that the protein fraction 2 at a concentration of 10 mg/ml, 5 mg/ml, 2.5 mg /ml had antimicrobial activity against E. aerogenes ATCC-13048, E. coli Mordovia 21. Acute toxicity of this fraction was assessed on laboratory mice after a single intragastric and parenteral administration. The average lethal dose for intragastric and parenteral administration for 20% solution of protein fraction 2 could not be established, since the maximum possible doses for these types of administration did not lead to death of any animal. It was established that 20% solution of protein fraction 2 belongs to the 4th hazard class (substances of low hazard) according to the generally accepted hygienic classification.