THE INTESTINAL MICROBIOTA AND SENSITIZATION TO COW’S MILK PROTEIN AS TRIGGERS OF FUNCTIONAL DISORDERS OF THE GASTROINTESTINAL TRACT IN CHILDREN IN THE FIRST SIX MONTHS OF LIFE
Functional disorders (FD) of the gastrointestinal tract - a common problem in children during the first months of life, the mechanism is not completely clear, discussed the important role of the intestinal microbiota and inflammation. The aim of this work was a comprehensive assessment of the intestinal microbiota and sensitization to cow’s milk protein in healthy children of first six months of life, depending on the type of feeding. Material and methods: 80 healthy children: 20 breast-fed, 60 (30 received mixture “Nestozen with probiotics”, 30 mix without Pro - and prebiotics -) were included in the study at age 2-3 months. was observed within 3 months. At baseline and after 3 months. studied the levels of calprotectin, short-chain fatty acids in feces, IgE and IgG4 to cow’s milk protein in coprofiltrates and sowing of a feces on a dysbacteriosis Results: the short-chain fatty acids Level did not correlate with the data of microbiological studies of feces. Dynamics of short-chain fatty acids in the two groups were similar, but the level of all short-chain fatty acids was higher in the group receiving the mixture of probiotics (9,12±1,56 mg/g), significantly higher than the group without probiotics (4,2±0.57 mg/g). About 70% of children bottle-fed were sensitized to cow’s milk protein, for 7 months. the level of IgE was significantly reduced. The average level of calprotectin was reduced in all groups and had significant differences, but a comparison of every patient found a strong relationship between high calprotectin level and high IgE levels and also too high (above 9 mg/g) or too low (less than 2.9 mg/g) level of short-chain fatty acids Conclusion: the study confirmed the role of the functional state of the intestinal microbiota and sensitization to cow’s milk protein in the development of intestinal inflammation. A mixture of “Nestozen with probiotics” have a preventive effect due to the formation of a more Mature microbiota, anti-inflammatory effect and normalization of intestinal functions