TRANSCRIPTOMIC ACTIVITY OF ESTROGEN-REGULATORY GENES IN MALIGNANCY UTERINE TISSUES
Uterine cancer is the sixth on prevalence of cancer among women in developed countries. Hyper-estrogen level by endogenous or exogenous origin plays the leading role in this disease pathogenesis. This study purpose was investigating the expression of genetic loci responsible for the estrogen reception and metabolism to better understanding of the molecular mechanisms of uterine tissues malignancy. We investigated the relative expression of genes (CYP1A 1, CYP1A 2, CYP1B 1, CYP19A, ESR1, ESR2, GPER, STS, SULT1A and SULT1E1) by real-time PCR in tumor and conditionally healthy uterine tissues of 27 female patients in Southern Russia aged 38-72 years with histologically confirmed diagnosis of uterine cancer. It is found that transcription activity of these genes during malignancy of uterine tissues depends of female patients age and the stage of tumor cells differentiation. It is found that malignancy change in the transcriptional activity of investigated genes in uterine tissue depends of patients age and stage of tumor cells differentiation. Particularly, it was found, that changes of gene transcription balance of enzymes that provide sulfation and hydrolysis of steroid sulfates is depending of patient age and the degree of tumor cells differentiation. In addition, the sinking of gene expression of nuclear estrogen receptor ESR1 as lowering cell differentiation of tumor tissues. We have also shown gene expression of nuclear estrogen receptor ESR1 decline as lowering cell differentiation of tumor tissues