DEVELOPMENT AND VALIDATION THE METHOD OF QUANTIFICATION OF FLAVONOIDS IN MEADOW SWEET FLOWERS BY LIQUID CHROMATOGRAPHY
Simple and fast procedure for identification and assay of flavonoids in Meadow sweet flowers (Filipendula ulmaria L.) by HPLC is described. For the determination a 40% alcohol and RP column (Zorbax SB C-18 250×4,6 mm, 5 mcm); a mobile phase 0,01М potassium dihydrophosphate (рН=3,0) and acetonitrile in the ratio 80:20 (v/v) (isocratic elution); column temperature 30°С are used.