Development of soybean somatic embryos under different light spectra
Soybean (Glycine max (L.) Merr. cv. 'Primorskaya 28') plantlet development from somatic embryos (embryoids) under different spectra was studied. Light irradiation with intensity of 48 μmol/(m2·s) was generated by LEDs (light emitting diodes) with various spectra: cold white (CW), white (W), warm white (WW), full spectrum (FS, 450 nm and 660 nm), red (R, 630 nm), blue (B, 440 nm), and sun box (SB, close to the sun spectrum at wavelength range 440-660 nm). Fluorescent lamps were used as a control illumination (K). Our results showed that experimental LED lights with different spectra possessed the potential to positively affect the development of soybean plantlets from embryoid phase to a full-fledged plantlet, ready to be transferred into soil. The best effect was observed for the FS, which provided development of the highest plantlets. SB light was promising after one month of cultivation, but provided no significant differences in plantlets growth at the end of the experiment. A tendency to the lower growth was observed for plantlets under CW, W, WW, and K. Red light did not provide notable shoot elongation, as was supposed to. Investigations will be continued to clarify and refine the obtained data on the effect of light spectra on soybean plantlet development from embryoids.