ANTIMICROBIAL EFFICIENCY OF NIOSOMAL FORMS OF DEFENSINS UNDER THERMAL EXPOSURE
Endogenous defensins are a promising alternative in the treatment of infectious processes caused by antibiotic-resistant microflora. The effect of heat treatment on the dispersed composition of nanovisicles during the preparation of niosomal forms of defensins was considered. When analyzing the data of electron and atomic force microscopy, as well as the constructed integral and differential curves of the distribution of nios by size, the influence of the temperature effect on the value of their equivalent diameter was confirmed. Comparison of different temperature regimes of exposure to organosilicon niosomes showed that when they are heated, homogenization of the size of the vesicles occurs. With an increase in temperature, the number of niosomes less than 100 nm in diameter in the gel increased: from 48,8% at 25° C to 89,4% at 40° C. A decrease in the size of niosomes under temperature influences during their preparation increases their effectiveness against microorganisms resistant to antibiotics. The disk diffusion method has demonstrated the correlation of the size of the growth inhibition zone of antibiotic-resistant microorganisms around the discs with niosomal defensins when exposed to different temperatures: 25° C, 30° C, 35° C, 40° C.Statistical analysis has confirmed the ability to control the size of niosomes by heat to increase their antimicrobial efficacy.