DnaK Сhaperone Takes Part in Folding but Not in Refoldingof Thermal Inactivated Proteins in Bacillus subtilis
The role of DnaKJE and trigger factor (TF) chaperones in folding and refolding of proteins in Bacillus subtilis is studied. Bacterial luciferases of Photobacterium leiognathi and Photorhabdus luminescens as protein substrates have been used. It is shown that DnaKJE takes part in folding but not in refolding of the thermal inactivated proteins. It is shown that the TF take part in the synthesis of thermolabile P. leiognathi luciferase, but significantly decreases the level of synthesis of thermostable P. luminescens luciferase.