DEVELOPMENT OF A BIOSENSOR BASED ON IMMOBILIZED HORSERADISH PEROXIDASE ISOLATED FROM THE ROOTS OF ARMORACIA RUSTICANA
The bioreceptor element of the biosensor was formed by immobilization of the horseradish peroxidase enzyme into a sol-gel matrix based on polyvinyl alcohol. Operational stability, long-term stability, and the Michaelis constant of immobilized horseradish peroxidase were determined. Hydrogen peroxide was used as the analyzed compound, measurements were carried out on an amperometric biosensor installation of a cuvette type with an oxygen electrode of the Clark type.