MODERN TECHNOLOGIES OF BRAIN STEM CELLS CULTURE
The article describes the characteristics of neuronal stem cells, features of the neurogenic niches formation in the adult brain, and also provides methodological approaches to the isolation and culturing of brain stem cells. The main focus of the article is a description of the achievements of microfluidic technologies for the three-dimensional culture of neuronal stem cells and the establishment of the neurogenic niche model. The microfluidic approach offers a unique opportunity to get a 3D cell culture by creating a platform for developing complex and dynamic microlevels that can be monitored, reproduced, optimized and used to simulate in vivo conditions.