BACTERIAL CONTAMINATION AS A FACTOR OF ENTEROVIRUS INFECTION DETERIORATION (ACCORDING TO THE MATERIALS OF OUTBREAK THAT OCCURRED IN A DAY CARE CENTER "TEREMOK" IN ROSTOV-ON-DON CITY)
A total number of 35 people from the enterovirus infection outbreak were under surveillance including 15 children hospitalized in the Central municipal hospital named after N.A. Semashko of the Rostov-on-Don city and 15 children treated in the outpatient clinic № 5 as well as 5 exposed people - employees of the day-care center “Teremok”. The laboratory analyzed 53 biological samples collected from oral pharynx and nasal pharynx. The material was collected and delivered in the laboratory within 2-3 hours after the sample collection. Detection and identification of the microorganisms was conducted by means of differential-diagnostic medium and incubation with carbon dioxide. The reaction of latex-agglutination (Pastorex meningitides BIO-RAD) and mass-spectrometry analysis using Bruker Daltonik MALDI Biotyper were performed. Susceptibility to antibiotic drugs was identified with the aid of disc-diffusion method. S. pneumoniae was identified in majority (80%) of hospitalized children diagnosed with enterovirus infection. Those children that were treated in an outpatient clinic S. pneumoniae was diagnosed in lesser extent (47,6%). Similarities of isolated S. pneumoniae strains according to the antibiogram profile and mass-spectrometry profile matches evidence for a single source of origin.