EXPLORE THE POSSIBILITIES FOR USING NANOMATERIALS IN BIOTECHNOLOGY OF DRUGS FOR THE PREVENTION OF ANIMALS ANTHRAX
The aim of the research was an experimental study the possibility of using nanosized materials in a range in the design and manufacture of veterinary drugs for the prevention of animal's anthrax. Materials and methods: During the study used bacteriological, biochemical, physical, chemical, cultural, microscopic, biological and statistical research methods. There were used certified strains, strains deposited in the Depositary of State Scientific Control Institute of Biotechnology and Strains of Microorganisms: Bacillus anthracis Sterne 34F2, Bacillus anthracis M-71. Standardised not mutagenic, non-genotoxic and biosafety solution of colloidal gold containing nanoparticles was produced at the Institute of Chemistry of colloid F.D. Ovcharenko by original method of T. Gruzina and L. Ryeznichenko. In studies using gold nanoparticles of (19,0 ± 0,9) nm till (30,4 ± 0,5) nm at a final concentration of 19 ± 2 mg/ml. Results and discussion: The vaccine Sterne 34F2-Au / M and Sterne 34F2 vaccine protected 90 % of animals, where as vaccine Sterne 34F2-Au protected 100 % of animals from death. Because guinea pigs used in determining the immunogenicity after inoculation drugs they were alive in 100 % of cases - this indicates a safety test specimens. Conclusions and recommendations for further research. Thus, as a result of laboratory studies have shown the use of gold nanoparticles in the design of vaccines against anthrax gold nanoparticles animals with strain Sterne 34F2. In experiments with laboratory animals found that the immunogenicity of experimental samples ranged within 90-100 %. In this case, the highest percentage of immunogenicity was observed in a sample of the vaccine, which has been linked gold nanoparticles during formation of a series of drug (100 %). Made drugs meet all indicators of safety and quality that are used for this kind of drugs. These results provide an opportunity for further research to study the safety and efficacy of experimental drugs in terms of households. Promising seen further research on the use of nanoscale materials consisting of veterinary immunobiological agents against other infectious diseases.