The effects of ultraviolet laser radiation on macrophages functional activity
The aim of the research. To assess the effects of UV laser radiation on macrophages functional activity. Plotting the dose-response curves characterizing effects of UV laser radiation on the activity of peritoneal and splenic macrophages in vitro; comparing the response of peritoneal and splenic macrophages to UV irradiation. Materials and Methods. Irradiation of mouse peritoneal and splenic macrophages in vitro has been performed with the pulsed Nitrogen laser (l=337 nm) at various doses. Assessment of macrophages functional activity was done with the standard NMT-test. Non-irradiated cells have been used as a negative control. Results. We found that UV laser radiation affected functional activity of peritoneal and splenic macrophages in vitro with the stimulatory effect of low and inhibitory effect of high doses of radiation. Macrophages of peritoneal cavity and spleen demonstrated heterogeneity in their response to the action of similar radiation doses. The “dose-response" curve to characterize the UV-induced effects on peritoneal and splenic macrophages was plotted. Conclusion. Using of the model system (macrophages) allowed demonstrating that NADPH-oxidase is the main molecular target for the action of UVlaser radiation (l=337 nm). Lower doses of radiation (160 mJ/ml) stimulate the activity of macrophages, while higher doses (3840 mJ/ml) suppress it, and peritoneal macrophages are more sensitive to the stimulatory effect of UV laser radiation. Our findings could be used to predict the effects of UV-based fluorescent optical biopsy or PUVA-therapy.