EVALUATION OF THE DYNAMICS OF BLOOD LOSS INDICATORS WHEN USING NEW SAMPLES OF LOCAL HEMOSTAPTIC AGENTS AFTER LIVER INJURY IN THE IN VIVO EXPERIMENT
Currently, surgery of parenchymal organs uses various options for local hemostatic agents, the development and testing of which is one of the promising areas. Objective - to evaluate blood loss after liver injury (by examining changes in clinical blood counts) and the use of new samples of local hemostatic agents in a chronic in vivo experiment. Methods and materials. The study was performed on 20 male rabbits, which were modeled laparoscopically with a lacerated wound of the liver. Bleeding was stopped by applying local hemostatic agents based on the sodium salt of carboxymethylcellulose: Na-CMC (group 1, n = 10), Na-CMC + tranexamic acid (group 2, n = 10). Before surgery, on the 1st, 3rd, 7th, 15th, and 30th days after the simulation of liver injury, the parameters of the general clinical blood test were evaluated for the laboratory animal, and the volume of blood loss was calculated using the hematocrit method of F.D. Moore (1956). Differences between groups were determined using the nonparametric Mann-Whitney test and were considered significant at p < 0.05. Results. During the study, there was a slight decrease in the indicators of «red blood» (hematocrit, hemoglobin, erythrocytes): in rabbits of group 1 - on the 1st day after liver injury, in rabbits of group 2 -maximum on the 3rd day. Subsequently, there was a gradual increase in the studied parameters to the initial values by the day 15-30. There were no significant differences between the groups in terms of the listed parameters at different periods of observation. However, the calculated volume of acute blood loss in animals of group 1 was 2.4 times higher than in group 2: 5.68 [5.24; 11.39] ml and 2.35 [2.01; 3.13] ml, respectively. Conclusion. The data obtained indicate the prospects for further studies of local hemostatic agents based on the sodium salt of carboxymeth-ylcellulose.