Molecular mechanisms of non-specific tolerance to insecticides in colorado potato beetle: activation of genes transcription of receptor and store proteins
Summary Spreading of resistant populations of insect pests and detection of resistance toward a new chemical preparations as well as development of resistance to insecticides of biological origins are considering the critical problem in the field of crop protection but also of crop storage. Facts of extension of resistant species and populations area which has been accumulated during past years are not in accordance with conception of reduced fitness in resistant individuals. The necessity is obvious to investigate the non-specific tolerance which is based, as was supposed until recently, on the constitutive peculiarities providing the certain level of xenobiotic resistance. The phenomenon has been revealed of expression enhancement for a number of genes coding proteins under insecticide impact in different orders of insect pests. The aim of our investigation was determination of distinctions in transcriptional activity of genes associated with diapause in Colorado potato beetles Leptinotarsa decemlineata Say not exposed to insecticides in summer and resistant ones survived after treatments by different chemical compounds: organophosphate pirimifos-methyl, neonicotinoid tiamethoxam, or phenylpyrazole fipronil. Adults from the excerpt of Colorado potato beetle summer generation in Bashkortostan Republic entered the winter diapause under laboratory conditions. At the phase of diapause termination the analysis of transcriptional activity performed for a set of genes using real-time qPCR method. In the article data presented of enhancing of transcriptional activity of some receptor and store proteins genes under insecticide influence in Colorado potato beetle adults from local population with detected multiple resistances. The hypothesis has been discussed concerning the participation of proteins coding by gene of ecdysone receptor ecr, gene of subunit of gamma-aminobutyric acid receptor GABA-A rdl, gene of store diapause protein dp-1, genes of transport proteins fer and jhbp in immobilization and deposition of insecticide molecules during winter diapause which may present one of molecular mechanisms of non-specific tolerance to insecticides.