Changes in the immunoreactivity of ATP-synthase in neurons of cerebral cortex and cerebellum of rats with cholestasis
Objective. To study of the possible role of ATPase in cholestasis studied the content of ATP synthase in neurons of the cerebral cortex (frontal and parietal), Purkinje cells of the cerebellum at different times of experimental cholestasis in rats. Methods. The study was performed on 60 outbred white male rats weighing 200-250 g. Experimental animals were subjected to the common bile duct ligation, control - a sham operation, when there have a physiological bile outflow to the duodenum. Results. It was found that after the common bile duct ligation, expression in the pericarions of neurons of the cortex and in the Purkinje cells of the cerebellum changes wavy: in the frontal cortex of the brain in the pericarion of the neurons, it is increased by 2-5 days, then by a decrease (minimal - on the 10th day) and normalization from the 45th day. In the parietal cortex: increase by 2-5 days, then, decrease (minimal - on the 10th day), however, the normalization only to 90 days. In Purkinje cells - on the 2nd day - an increase, and from 5 to 20 days of the experiment - a decrease in expression (minimum values on the 5-10th day), from the 45th day the normalization of this indicator was revealed. Conclusions. After the common bile duct ligation, the content of ATP synthase in the perikaryons of neurons of the cortex (frontal and parietal) and in the Purkinje cells perikaryons of the cerebellum changes wavy: it is increased in conditions of cholestasis and decreased in conditions of its elimination.