Study of Motherwort herb BAS dynamic extraction for obtaining the tincture
Topicality. Motherwort herb tincture is one of the most popular herbal remedies of sedative activity. Tincture is obtained by maceration in the ratio of 1 : 5. In the waste of the Motherwort herb there is still a significant amount of BAS. Taking this into account, it is advisable to investigate the dynamics of BAS extraction from the Motherwort herb and determine its optimal multiplicity. Aim. To study the Motherwort herb BAS dynamics extraction by 70 % ethanol and to determine the optimal multiplicity in obtaining tincture from this raw material. Materials and methods. Determination of hydroxycinnamic acid derivatives, flavonoids and henolic compounds was performed by spectrophotometric method and HPLC method. In order to optimize the extraction of BAS and to select the optimal extraction multiplicity, the coefficient of mass return in each stage (mi extract/mi ethanol) for each of the indicators was calculated, the dependence of these factors on the extraction multiplicity to determine the optimal extraction multiplicity was deduced from the application package. Results and discussion. As a result of the HPLC analysis of Motherwort herb extracts, 5 substances were identified: 2 flavonoids (routine and catechin) and 3 hydrocinnamic acids (chlorogenic, coffeic and rosemarinic), and their quantitative content was determined. In the largest number of extracted extracts contained chlorogenic acid and routine. In the obtained extracts from the ethanol by spectrophotometric method, the content of the main groups of BAS was determined: hydroxycinnamic acids, flavonoids and the sum of phenolic compounds. According to the results of the research, the equation of dependence of the profitability of alcohol extraction from Motherwort herb on the extraction of the extractives, the amount of phenolic compounds, hydroxycholic acids, flavonoids and corresponding graphs were constructed. Conclusions. The study of qualitative composition and quantitative content of the main groups of BAR (flavonoids, hydroxycinnamic acids) in extracts from Motherwort herb and using mathematical methods showed that the optimal multiplicity of extraction of BAS from this raw material for the manufacture of tincture is twice.