THE PECULIARITIES OF THE EMBRYOTOXIC ACTION OF DIISONONYL PHTHALATE IN EXPERIMENTS ON LABORATORY ANIMALS
The study of the peculiarities of diisononyl phthalate toxic effects on laboratory animals showed the initiation of teratogenic effects in rats on intragastric administration to females during the gestation period. The exposure level of 10,000 mg/kg of phthalate was characterized by an increase in total postimplantation and embryonic mortality, the presence of multiple (combined) malformations of embryos. Postnatal development of the offspring was characterized by the disturbance of the endocrine system function: the thyroid and sex glands, leading to the development of primary testicular insufficiency and thyrotoxicosis. In the experiment, the most inactive dose was 10 mg/kg. Elements of the embryotoxic action mechanism of diisononyl phthalate are the disturbances in the processes of cell differentiation, DNA damaging effect and cytotoxic properties of phthalate, which led to the formation of polyploid cells in the spleen on single intraperitoneal impact of phthalate in experiments on white mice, and accumulation of peripheral blood leukocytes with disorders of the genetic apparatus and differentiation (accumulation of segmentated cells, micronuclei, cells with the signs of necrosis) on subchronic intragastric introduction to male white rats.