THE FUNCTIONAL VALUE OF ERYTHROCYTIC PROTEINS IN SERUM AND THEIR CONTENT
Erythrocytic proteins entering the environment when incubating red blood cells, take part in the regulation of erythropoesis. However, their role in this process is unclear. One of the approaches to the solution of this issue may be a study of the quantitative content of these proteins in the serum in conditions characterized by activation or suppression of erythropoies. Methodology. Experiments were performed on 74 white non-breeding rats - mass 50-70 g. Red blood cell extracts containing red blood cells, received by incubation of red blood cells rats. The resulting extracts immunized 3 rabbits weighing 2.5-3 kg and prepared anti-collar. The reaction of the agar in Oulterloni was carried out. As an antigen (AG) to the rabbit antiserum we used serum intact rats, rats after acute blood loss, polycythemia, the introduction of extracts of red blood cells rats, as well as extracts of red blood cells, bone marrow and thymus. Polystememia in rats reproduced by transfusion method, introducing homologous red blood cells in the amount of 2% of body weight, acute blood loss caused bloodletting in the volume of 2% of body weight. Bone marrow and thymus extracts were obtained under the same conditions as red blood cell extracts. Results and discussion. The results showed that red blood cells are released into the environment not only in vitro, but also in vivo. Their content in different extracts depends on the number of red blood cells among incubated cells. As the inhibitory properties of red blood cell extracts are associated with the presence of keilons in them, it can be assumed that the activation or suppression of erythropoese in functional states (blood loss, polycythemia) depends not only on erythropoietin, but also on the amount of red blood cells circulating in the blood.