PATHOLOGISTOLOGICAL CHANGES IN THE GASTROINTESTINAL TRACT OF PIGLETS INFECTED WITH AUJESZKY’S DISEASE
Introduction. Aujeszky’s disease is the acute contagious disease manifestating as epizootic or sporadic outbreaks. It causes significant economic losses to the livestock husbandry especially in the countries with intensively developed pig and fur animal husbandry.Scientists studying Aujeszky’s disease have described in detail the pathological and anatomical changes in pigs under course of the disease but there is insufficient data on microscopic changes of internal organs of suckling piglets died from this disease. So we studied microscopic changes in the stomach and intestines of suckling piglets, who died from Aujeszky’s disease. It was important to study the histological changes in the internal organs of suckling pigs, due to the significant variability of the etiological factor of this disease, namely Suis alphaherpesvirus 1.The goal of the work was to investigate the microscopic changes in the stomach and intestinal system of suckling piglets died from Aujeszky’s disease.Materials and methods. For histological studies, samples of the gastrointestinal tract of suckling piglets died from Aujeszky’s disease were collected. Organ tissue samples were taken from 5 suckling piglets of 4 to 6 days old. Diagnosis was confirmed by polymerase chain reaction in all piglets.The sampling was carried out no later than 1 hour after the death of piglets. The pathocogical matherial was fixed in a 10% solution of neutral formalin subsequently in ethanol of increasing concentration and embedded in paraffin through chloroform.Sections of 7-9 μm thickness were obtained on a sled microtome, and stained with Karazzi hematoxylin and eosin. The preparations were studied using a binocular light microscope.Results of research and discussion. As a result of histological examination of the internal organs of the gastrointestinal tract of piglets died from Aujeszky’s disease, we found the presence of significant microscopic changes in all morphological formations of these organs.Conclusions and prospects for further research. It was revealed that features of Aujeszky’s disease on microscopic level were the presence of intranuclear eosinophilic insertions in the epithelial cells of the stomach, enterocytes of the villi of the small intestine and enterocytes of crypts of the cecum and colon. Also it was detected the pathological changes: in the stomach - the presence of cellular syncytium in the gastric dimples, metaplasia of smooth muscle cells of the muscular membrane and the mesotheliocyte of the serous membrane in the small intestine and expressive hypertrophy of the fibroblasts of the mucous membrane in the cecum and colon.