DETERMINATION OF HISTOCOMPATIBILITY ANTIGENS FOR ORGAN AND TISSUE TRANSPLANTATIONS. DETECTION OF A NEW ALLELE HLA-DQB1*03:82
The study shows the descriptionof a new allelic variant of the HLA-DQB1 gene, first identified in the Republic of Kazakhstan. From 2011 to 2015 at the Scientific-Production Center of Transfusiology of Astana city,3501 potential donors for blood stem cell transplantation for the National Register of Blood and 936 patients with oncohematological diseases (aged 0-66 years) were analysed.During the allele-specific sequencing of Kazakhstani oncohematological patient with acute myeloid leukaemia (AML), a new allelic variant of HLA II class DQB1*03:82 lociwasdetected.The novel allele is similar to the existingHLA-DQB1*03:01 with a single non-synonymous difference, at position 223 peptide binding grooveG>A (codon 79 TGC to TAC). It is shown that this novel allele has been inheritedand was not because of mutation in AML.