PECULIARITIES OF SUBPOPULATION COMPOSITION OF IMMUNOCOMPETENT CELLS IN PLACENTAS AFTER PREMATURE DETACHMENT
Aim. To assess the peculiarities of CD20+, CD4+, CD8+, CD56+ expression in placentas after premature detachment. Materials and methods. Histological material of placentas was divided into 4 groups. Group 1 ( n = 9) - placentas after premature detachment in the gestation period from 22 to 27 weeks. Group 2 ( n = 9) - placentas after premature detachment in the gestation period from 28 to 36 weeks. Group 3 ( n = 9) - placentas after premature detachment in the gestation period from 37 to 42 weeks. Group 4 ( n = 15), the control group - placentas of conditionally healthy women with uncomplicated pregnancy and labor. Immunohistochemical study included determination of CD20 (clone L26), CD56 (clone 123C3), CD8 (clone LT8), CD4 (clone W3/25) expression. Results. While analyzing the expression of CD20+ (B-lymphocytes) differentiation cluster in placentas after premature detachment, increase in their quantity is reliably more often assessed in groups 1 and 2. In the study conducted by us, there is statistically reliable difference in the expression of CD4+ differentiation cluster in placentas of women with premature detachment of placenta in the gestation period from 27 to 36 weeks, 19,2 ± 2,4. In the group of women, whose pregnancy resulted in premature detachment of placenta in the gestation period beginning from 37 weeks, this parameter is equal to 3,2 ± 1,1 that significantly differs from the group of conditionally healthy women. It is evident that premature detachment of placentas in the gestation period from 28 to 36 weeks CD4/CD8 index is significantly elevated till 5,33 at the expense of increased T-helpers, whereas in the other cases it is 0,75. Conclusions. 1. Irrespective of pathogenetic aspects predisposing to premature detachment of placenta, the number of NK-cells is growing in all placentas of women with this pathology: group 1 - 4,8±1,73, group 2 - 4,8 ± 1,2, group 3 - 6,4 ± 1,4, vs control - 1,4± 0,6. In groups 1 and 2, CD20+ expression is reliably more marked: 3,6 ± 1,73 and 3,3 ± 1,1, respectively, vs 1,0 ± 0,66 in group 3 and 0,8 ± 1,06 in the control group. In placentas of group 2, expression of CD4+ differentiation cluster is statistically significantly strengthened: 19 ± 2,4, vs 3,1 ± 0,6; 2,4 ± 0,53 and 4,6 ± 1,7 in groups 1, 3 and 4, respectively.