Study of Expression of Human Glioma Genetic Markers under Different Degrees of Gliomas Malignancy
In view of increasing necessity to establish the genetic characteristics of glioma stage, expression dynamics of selected tumor markers as well as markers of stem cells and neural differentiation were investigated. 27 samples of human gliomas of 2-4 stages and cell cultures from these samples were studied. The study results demonstrate how the expression profile of selected genes changes from the initial phases of the disease to its final stages both in humans and in glioma cell cultures in vitro. It was concluded that glioma cell cultures could be used as models for glioma studies, but results of these studies must be applied with caution because of heterogeneity of both the gliomas and cell cultures derived from gliomas tissue. Based on obtained data, authors offer a range of factors, which may predict poor clinical outcome.