The influence of vitamins-antioxidants C and E on free-radical processes in the blood of rats
The purpose of the research was to study any possibilities to prevent the oxidative stress development in the blood of rats under short-time hypothermia (30ºC) by injecting vitamins C and E. The testing was performed with Wistar male rats weighted 200±5 g. The animals’ body temperature was reduced to 30ºC (short-time hypothermia). The animals were given single intraperitoneal vitamins C and E injections within seven days. To the first group alpha-tocopherol (Codex E-Selenium) was administered in the dose of 40 mg per kg of the body weight, to the second group ascorbic acid was injected (Codex drug, lyophilized, for injections) in the dose of 100 g per kg of the body, to the third group alpha-tocopherol and ascorbic acid were introduced together in the same doses. The lipid peroxidation (LPO) intensity in erythrocytes was assessed by malondialdehyde (MDA) contents. The oxidative protein modification intensity in erythrocyte membranes was assessed by carbonyl groups contained therein and reacting with 2,4-dinitrophenylhydrazine. In erythrocytes the contents of reduced glutathione (GSH), the activity of superoxide dismutase (SOD) and catalase were determined. It was found that the combined course vitamin C and E introduction prevented the FRP activation in blood and protected the erythrocyte membrane from oxidative affection under short-term moderate hyperthermia.