Study of cytokine therapeutic effect of dyalderon in the scheme of treatment of cattle for disease caused by Pseudomonas aeruginosa
The article covers information on the study of cytokine therapeutic effect of dyalderon on some interleukin indices and other nonspecific immunity factors in Pseudomonas aeruginosa-infected cattle. The research was conducted on calves aged 2-2.5 months. They were divided into 3 groups 20 animals each, according to analogue principle. The first group included clinically healthy animals. The calves of the 2-nd experimental group ill with pseudomonosis were treated using antibiotics and symptomatic medications according to the following scheme: in a day they were administered tobramycin sulfate in a dose of 0.5 mg/kg per day during a week, the next day they were given azithromycin (Azitronite) at a dose of 2.0 ml, intramuscularly, during a week, in addition, they were given trivitum at a dose of 2.0 ml intramuscularly, 450 ml of 5% glucose in normal saline intravenously. Pseudomonosis-infected calves of the 3-d experimental group in addition to antibiotic therapy were given dyalderon at a dose of 200 mg per 1 head intramuscularly, once a day during 7 days. On the fifth day animals of the 3 -d experimental group showed absence of laryngotracheitis and bronchitis symptoms as opposed to the 2-nd experimental group calves. On the 18-th day calves of the 3-d experimental group showed normalization of white blood cells, lymphocytes and red blood cells count, ESR (blood sedimentation rate) decreased from 27.8±0.04 to 9.2±0.52 mm/h (Р<0.05), the level of non-specific immunity factors in blood serum increased: interleukin-2, interleukuin-4 (from 1.6±0.02 to 5.4±0.18 pg/ml) and interleukuin-6 (from 9.2±0.08 to 30.4±0.02 pg/ml) to normal physiological indices of healthy animals. It was proved by strengthening gammaglobulin synthesis in calves (54.8±0.5 mg/dL), by rise in cytolytic and phagocytic activity of alveolar macrophages and leucocytes and by suppression of Pseudomonas aeruginosa toxin synthesis, that led to death of Pseudomonas aeruginosa in calves after combination therapy with antibiotics and dyalderon. Pseudomonas aeruginosa culture was not isolated from the biomaterial of 3-d experimental group animals as opposed to the calves of the 2-nd experimental group.