EFFECT OF CONCENTRATION OF SUSPENDED TEST-CULTURE AND INCUBATION TIME OF SAMPLES ON THE VALUE OF BACTERICIDAL ACTIVITY OF SALIVA AND BLOOD SERUM OF COWS AND SHEEP
Types of microbes and concentrations of suspensions test-culture, growth phase of test-culture and incubation time of samples have a big impact on the value of bactericidal activity. The effect of the above factors on the value of bactericidal activity of saliva is unexplored. According to our data increasing the concentration of test-germs of E. coli and M.lysodeikticus introduced into the sample to 150 millions bact. cells reduced the value of bactericidal activity of saliva and serum. Increasing of incubation time of samples from 3 to 5 h lead to the raise of the value of bactericidal activity of saliva and serum when using E.coli and to the drop in its value when using M.lysodeikticus. Wnen introducing into the sample to 5 millions bact. cells of B subtilis the value of bactericidal activity of saliva and serum was 100 % herewith the growth of the optical density into the check samples practically not observed. Wnen introducing into the sample to 150 millions bact. cells of B subtilis with increasing of incubation time of samples the value of bactericidal activity of saliva grew, serum - decreased.