EFFECT OF COLD STRESS ON THE ACTIVITY OF CERTAIN ENZYMES OF RAT BLOOD
To determine the effect of cold stress on the activity of some enzymes in the blood of rats, the animals were divided into two groups: the first control group was kept in thermoneutral conditions, the second - an experimental one, was placed outside at a temperature of -4°C for 30 and 80 minutes. During the study, it was observed that in the blood plasma of rats, the activity indicators of the following enzymes: alkaline phosphatase, acid phosphatase, alanine aminotransferase, aspartate aminotransferase and creatine kinase, depending on the time of exposure to cold, change in different ways. After exposure for 30 minutes, a decrease in the activity of alkaline phosphatase and alanine aminotransferase was observed, while aspartate aminotransferase and creatine kinase, on the contrary, increased. Then, within 80 minutes, the activity of alkaline phosphatase continues to increase 1.7 times, creatine kinase twice. During the exposure period, the activity of acid phosphatase remained almost unchanged. Thus, as a result of research, it was revealed that the manifestation of the action of hypothermia is characterized by the predominance of changes accompanied by a decrease in bioenergetic processes in animals.