The assessment of ISSR markers application to genetic typing and certification of Vaccinium plants
Genotypes of four Vaccinium corymbosum cultivars and two cultivars of V. angustifolium х V. corymbosum, along with wild individuals of V. myrtillus, were analyzed using five inter-simple sequence repeat (ISSR) primers (UBC 808, 818, 824, 845, 867). The primers produced 128 bands on the whole; 22.7 % of the bands were common to all blueberry cultivars. In the case of V. myrtillus, 68.0 % of its bands were identical with monomorphic bands in cultivated blueberries. The obtained sets of consistent bands can be considered as cultivar passports and they can be used for Vaccinium cultivars certification. Cluster analysis of genetic similarity between cultivars, based on ISSR profiles, is provided.