Optimization of adaptation to cell-free culture media and storage of canine and feline mycoplasmas
Within the framework of the project to simplify the various stages of cultural works with mycoplasmas of dogs and cats, we tried to standardize the adaptation of isolates and microbiological clones through passaging on cell-free culture media. Unfortunately, it was not possible to simplify this stage - full adaptation to the culture media did not occur even with relatively short intervals between reinoculation, the growth from passage to passage became less pronounced and gradually died away. It has been established that storage at a temperature of - 18 <sup>0</sup>C makes it possible to preserve a small part of viable mycoplasmas for a period of about 3 years. It was also shown that routine PCR for detection microorganisms of the genus Ureaplasma in the feline urine could be used to confirm the presence of ureaplasmas in cultures on liquid and semi-liquid culture media.