CONSTRUCTION OF A GENOMIC BAC LIBRARY FOR DEVELOPMENT OF CYTOGENETIC MARKERS IN ALLIUM FISTULOSUM L
We have created more than 800 BAC clones for а variety of A. fistulosum «Russkiy zimniy». Selection of BAC clones carrying the sub-telomeric repeat was made by polymerase chain reaction with primers for this sequence. Screening of the BAC library for the presence of species-specific repeat was carried out using cross-dot-blot hybridization with biotin-labehed Cot — 1 fraction of the A. fistulosum and A. cepa genomes. Four BAC clones with the sub-telomeric repeat were revealed and one with a species-specific sequence.