HYALURONIDASE ACTIVITY OF BLOOD SERUM IN PATIENTS WITH HEPATIC CIRRHOSIS
Objectives. To determine the level of blood serum hyaluronidase activity in patients with hepatic cirrhosis. Material and Methods. Blood serum hyaluronidase activity was defined by the rivanol clot method. Hyaluronidase activity determination is based on the formation of the clot of rivanol with hyaluronic acid which is in an inverse proportion to the depolymerization of the latter under the effect of hyaluronidase activity of various origin. The serum titer, in which the activity was determined, as well as the size of rivanol clot were estimated. 32 patients suffering from cirrhosis of the liver were examined. Severity class of the disease was by Child-Pugh A (n=5), B (n=12), C (n=15). 18 out of these cases (56%) were men and 14 (44%) women. The average age of the studied group made up 54+10,82 years. The control group included 24 donors from the blood transfusion station, 12 out of them (54%) were men and 10 (46%) women. The average age of the control group made up 40,5+10,1 years. Results. As a result of the study of blood serum hyaluronidase activity of the patients having hepatic cirrhosis it was established that the serum titer in which hyaluronidase activity was determinated in the patients with cirrhosis of the liver made up 1/8000-1/16000 which was 2-4 times higher than the maximum titer of serum in the group of donors (1/4000). It was determined that in patients suffering from hepatic cirrhosis on the 7th day of treatment a decrease of hyaluronidase activity (р<0,05) was observed. At the same time, the level of hyaluronidase activity despite the provided treatment was reliably higher than that in the donors (р<0,05).