MONITORING OF APOPTOSIS FACTORS IN SPLEEN AND LIVER OF RATS AT EXPERIMENTAL LIVER METASTASIS
Liver metastasis is a common cancer complication. Aim - studying the dynamics of TNF-α, cytochrome C and caspase-3 (C-3) in tumors (Ts), their paratumoral zones (PZt), liver metastases (MTS) and their parametastatic zones (PZm) at liver metastasis. Methods. The study included 43 white outbred male rats. 1, 2, 5 and 7 weeks after the beginning of the experiment, levels of TNF-α, C-3 (BenderMedsystems, USA) and cytochrome C (Cusabio, China) were studied in Ts, PZt, MTS and PZm by ELISA. Results. In liver MTS, levels of TNF-α decreased (by 6.7 times), and levels of cytochrome C increased (by 4.0 times). In PZm, reduction of cytochrome C (by 8.0 times) and C-3 (by 3.6 times) was accompanied by an increase in the TNF-α level (by 3.6 times).Ts, PZt and PZm of rats with combined MTS to the liver and lungs showed an increase of C-3 and a more significant increase in TNF-α. Conclusions. The increasing levels of apoptosis factors in neoplasms demonstrate their higher malignancy and ability to metastasize.