CHANGE OF THE AFFINITY OF THE TATA-BINDING PROTEIN TO OLIGONUCLEOTIDES CORRESPONDING TO TATA-BOXES IN HUMAN GENE PROMOTERS BEARING POLYMORPHISMS ASSOCIATED WITH HEREDITARY DISEASES
Single-nucleotide polymorphisms (SNPs) in the regulatory regions of human genes and, in particular, TATA-boxes, can alter the sensitivity to bacterial and viral infections and to pharmaceuticals used for their treatment. These SNPs are associated with the risk of widespread multifactorial human diseases, such as arthritis, hypertension, cancer, Alzheimer's disease, and others. Interaction of the TATA-binding protein (TBP) with TATA-boxes on TATA-containing promoters nucleates preinitiation complex assemblage, being the initial stage of transcription regulation. This study considers the change of ТВР affinity to the TATA-boxes of human genes MBL, <i>CYP 2A6, SOD1, </i>and TPI, which bear SNPs associated with autoimmune diseases, cardio-vascular diseases, amyotrophic lateral sclerosis, neurodegenerative disorders, hypersensitivity to bacterial infections, etc. In all cases a decrease in the affinity of ТВР to TATA-containing oligonucleotides is observed.