FEATURES REGENERATING HYALINE CARTILAGE TISSUE AFTER PLASTIC ARTICULAR SURFACE DEFECTS BY COMBINED TRANSPLANTS
Scientific research involved 2 stages: in vitro - the production of cellular-tissue graft based on allogenic demineralized “Lioplast” spongiosa and cells’ cultures obtained from the rabbit’s costal cartilage; and in vivo - the study of efficiency and biocompatibility of this cellular tissue product in the experiment on animals. Demineralized spongiosa produced by “Lioplast” technology is the best 3D carrier for combined cellular-tissue graft production taking into consideration its biological availability and judging by the results of different porous allogenic materials in vitro testing. The existence and viability of cells on 3D biocarrier was proved by raster and confocal electron microscopy. Comparative morphological analysis of defect plasty zone by combined grafts with both autologic and allogenic costal cartilage tissue cells demonstrated that morphological differences at the early stages of hystogenesis did not reveal themselves by the end of the experiment. By the end of the experiment both experimental groups demonstrated organotypical structure restoring of both subchondrial bone and articular hyaline cartilage. Microscopic picture of plasty zone in both groups of animals were similar.