Effect of immobilized interferon alpha-2b on functional activity of immunocompetent cells in vitro
Adding in vitro immobilized interferon alfa-2b in doses 15, 150 and 1500 IU/ml to the culture of human mononuclear cells had no significant effect on phagocytosis of neutrophils, synthesis of immunoglobulins and proliferative activity of lymphocyte. Reaferon EC was taken as a reference drug. In certain individual doses it decreased the lymphocyte proliferation and stimulation index of B-cells. Immobilized interferon alfa-2b, as well as the reference drug reaferon EC, had no effect on spontaneous and stimulated the production of IL-1β and IL-4, but increased the production of IL-2 and IFN-y without any additional stimulation by mitogen. The immobilized interferon alfa-2b was found to be more efficient than the reference drug reaferon EC in intensification of stimulated production of IL-2. The investigated drug (immobilized interferon alfa-2b) has reduced the functional activity of natural cell-killers mush more than the reference drug (reaferon EC).