Expression of intronic microRNA miR-153 in prostate cancer
Introduction. The search for new markers for early diagnosis of prostate cancer (PCa) is an urgent task. The most promising substrate for research are microRNAs. Materials and methods. Study is based on the results of bioinformatic analysis of mRNA and microRNA sequencing of 184 PCa samples and 50 samples of conditionally healthy prostate tissue. Results. Among 182 differential PCa in T2x PCa, four pre-miRNA host genes were identified: AMH (hsa-mir-4321), MYH6 (hsa-mir-208), MYH7 (hsa-mir-208b), PTPRN (hsa-mir -153-1). Only the expression of hsa-miR-153-3p significantly differed between the comparison groups. Nine transcription factors that can regulate the expression of this microRNA have been identified: AR, CREB1, CTCF, ERG, ETV1, GABPA, MYC, SUMO2, TRIM24. Subsequent co-expression analysis showed a significant positive correlation of hsa-miR-153-3p expression with the androgen receptor. Conclusion. MicroRNA miR-153 can be considered as a promising marker of early stages of prostate cancer. It seems important to study the level of miR-153 not only in biopsy material, but also in blood plasma, urine of patients with prostate cancer and hyperplasia.