THE QUALITATIVE AND QUANTITATIVE ANALYSIS OF VOLATILE COMPOUNDS OF THE HERB OF DAHLIA NYMPHAEALES OF KEN’S FLAME CULTIVAR
Many plants consist of volatile substances, these substances possess a variety of pharmacological effects on the human body, the main ones are analgesic, anti-inflammatory, healing, antimicrobial, soothing, and so on. The aim was to study the qualitative composition and quantitative content of volatile substances of the herb of Dahlia Nymphaeales of Ken’s Flame cultivar by chromatography- mass spectrometry. For the study an Agilent Technology 6890 gas chromatograph with the mass spectrometric detector 5973 was used. As the result of the research 46 substances have been determined, among them 43 substances have been identified. It has been found that sesquiterpinoids - α-kadinol, globulol, vulgarol A, spathulenol, allo-spathulenol were dominant among all groups of com- pounds identified. The quantitative content of this group was 21.9% of the total amount of compounds. The content of both sesquiterpenoids and fatty acids was the same (17.7% each). Sesquiterpinoids were represented by 10 compounds: aromadendren, allo-aromadendren, β-Elemen, β-farnezen, ger- macrene D, germacrene B, shyobunon, farnezen, δ-kadinen, β-sesquiphellandrene. Shiobunon prevailed among sesquiterpinoids (210.81 mg/g). The fatty acids were represented by 4 compounds: palmitoleic acid, palmitic acid, oleic acid, linoleic acid, among them palmitoleic acid (200.46 mkg/g) prevailed. The content of triterpenoids (β-caryophyllene, α-caryophyllene, squalene) was 9.11%. In addition to these compounds the essential oil of Dahlia Nymphaeales herb contained alkanes (8.7%), sesqui- terpene ketones (7.9%), diterpene alcohols (5%). Furthermore, the raw material accumulated a trace amount of alkenylphenols (1.6%), ketones (1.4%), sesquiterpene oxides, aldehydes and phenols (1.2% each), monoterpene alcohols (0.32%).