Effect of cyclobenzaprine on the dynamics of neuropathic pain syndrome development in Wistar male rats
Objective. To study the effect of cyclobenzaprine on the dynamics of the development of neuropathic pain syndrome (NPS) in Wistar rats. Material and methods. NPS was reproduced by cutting the left sciatic nerve in experimental and control animals at the level of the popliteal fossa under ether anesthesia. The severity of NPS in animals was determined within 30 days after transection of the left sciatic nerve and was evaluated in score according to the severity of autotomy on the operated limb (lesion of bones, phalanges and feet), the time of occurrence of autotomies, and the number of animals with autotomy. Cyclobenzaprine was administered intraperitoneally at a dose of 20 mg/kg for 10 days. Control animals under similar experimental conditions were injected with saline in the same volume. Results. The study of the dynamics of the development of NPS showed that after transection of the left sciatic nerve, the first signs of neuropathic pain appeared in the control group on the 2nd day (in 1 out of 12), and in the group of experimental animals on the 3rd day (in 1 out of 13). The severity of autotomy in both groups was 2 scores. Subsequently, in control animals, a gradual increase in the number of animals with autotomies and their severity was observed. On the 30th day of the experiment, the number of animals with autotomies was 83.33% (10 out of 12). In the experimental group, an increase in the number of animals with autotomies and the severity of autotomies was observed only after the abolition of the administration of cyclobenzaprine, i.e. on day 12 of the experiment. On the 30th day of the experiment, the number of animals with autotomy was 46.15% (6 out of 13). The maximum severity of autotomy in control animals was 7 scores, and in experimental animals - 4 scores. Conclusion. The administration of cyclobenzaprine for 10 days had an antinociceptive effect, delaying the development of NPS, reducing the number of animals with autotomy and the severity of autotomy.