EXPRESS VERSION OF DEFINITION OF CEFOPERAZONE IN URINE BY CAPILLARY ELECTROPHORESIS
It is developed a method for the quantitative determination of cefoperazone by capillary electrophoresis ("Capel-105"). In the concentration range between 0.5 - 20.0 µg is linear dependence of photometric signal from the peak area (R2 = 0.999). According to the results of research we propose an express technique for determining of cefoperazone in urine (sample volume - 0.1 ml) based on a direct extraction of the organic system (chloroform - isobutanol in the ratio 3 : 1) with further re-extraction of the substance to the working electrolyte, diluted with water 10 times, and electrophoretic study. It is shown that the conditions for sample preparation of the biological fluid allow to eliminate the influence of ballast substances and to carry out UV spectrophotometric detection effectively. It was held a validation assessment of methodology for determining the cefoperazone in the urine. The obtained results are within the acceptance criteria. The boundary detection is 5 µg of cefoperazone in 0.1 ml biofluid.