Are nucleosomes positioned at DNA replication origins?
['yeast origins are characterized by an asymmetric pattern of positioned nucleosomes flanking the ACS. The origin sequences are sufficient to maintain a nucleosome-free origin; however, ORC is required for the precise positioning of nucleosomes flanking the origin.', 'Here, we identify nucleosome occupancy as a likely candidate to set up ORI distribution', 'we demonstrate that open chromatin domains, characterized by nucleosome depletion, are preferentially permissive for replication', 'Nucleosome assembly of the template prevented DNA replication. Replication of chromosomes was severely inhibited at more than two-thirds of physiological nucleosome density']
['No, origins of replication occur in nucleosome-free regions in both budding yeast and Drosophila. Open chromatin domains, characterized by nucleosome depletion, are preferentially permissive for replication.']
['no']