Are Drosophila ultraconserved elements candidate ncRNAs?
['Highly constrained intergenic Drosophila ultraconserved elements are candidate ncRNAs.', 'Here, we report the discovery and characterization of UCEs from 12 sequenced Drosophila species. We identified 98 elements ≥80 bp long with very high conservation across the Drosophila phylogeny. Population genetic analyses reveal that these UCEs are not present in mutational cold spots. Instead we infer that they experience a level of selective constraint almost 10-fold higher compared with missense mutations in protein-coding sequences, which is substantially higher than that observed previously for human UCEs. About one-half of these Drosophila UCEs overlap the transcribed portion of genes, with many of those that are within coding sequences likely to correspond to sites of ADAR-dependent RNA editing. For the remaining UCEs that are in nongenic regions, we find that many are potentially capable of forming RNA secondary structures. Among ten chosen for further analysis, we discovered that the majority are transcribed in multiple tissues of Drosophila melanogaster. We conclude that Drosophila species are rich with UCEs and that many of them may correspond to novel noncoding RNAs.', 'Highly Constrained Intergenic Drosophila Ultraconserved Elements Are Candidate ncRNAs']
['Yes. Highly constrained intergenic Drosophila ultraconserved elements are candidate ncRNAs.']
['yes']