Does splicing occur co-transcriptionally?
['Researchers working in multiple model organisms - notably yeast, insects and mammalian cells - have shown that pre-mRNA can be spliced during the process of transcription (i.e. co-transcriptionally), as well as after transcription termination (i.e. post-transcriptionally)', 'The consensus view, based on four organisms, is that the majority of splicing events take place co-transcriptionally in most cells and tissues.', 'Deep sequencing of subcellular RNA fractions shows splicing to be predominantly co-transcriptional', 'We show that in the human genome, splicing occurs predominantly during transcription.', 'Consistent with co-transcriptional spliceosome assembly and splicing, we have found significant enrichment of spliceosomal snRNAs in chromatin-associated RNA compared with other cellular RNA fractions and other nonspliceosomal snRNAs. ', 'The majority of introns in higher eukaryotes are excised prior to transcript release in a manner that is dependent on transcription through pol II', 's a result of co-transcriptional splicing, variations in pol II elongation influence alternative splicing patterns, wherein a slower elongation rate is associated with increased inclusion of alternative exons within mature mRNA. ', 'We show that the pattern of intronic sequence read coverage is explained by nascent transcription in combination with co-transcriptional splicing', 'Modelling reveals co-transcriptional splicing to be the most probable and most efficient splicing pathway for the reporter transcripts, due in part to a positive feedback mechanism for co-transcriptional second step splicing', "RNA processing events that take place on the transcribed pre-mRNA include capping, splicing, editing, 3' processing, and polyadenylation. Most of these processes occur co-transcriptionally while the RNA polymerase II (Pol II) enzyme is engaged in transcriptional elongation", 'Abundant evidence indicates that splicing to excise introns occurs co-transcriptionally, prior to release of the nascent transcript from RNAP II', "Together, our work establishes a system for co-transcriptional splicing in vitro, in which the spliceosome containing the 5' and 3' exons are tethered to RNAP II for splicing.", 'Co-transcriptional splicing of constitutive and alternative exons', 'Current evidence supports co-transcriptional spliceosomal assembly, but there is little quantitative information on how much splicing is completed during RNA synthesis', 'Thus, we demonstrate that the decision to include or skip an alternative exon is made during transcription and not post-transcriptionally', 'Here, we demonstrated that the co-transcriptional splicing of the intron in vitro was blocked by antisense oligonucleotides (AONs) targeting the P3-P7 core of the intron', 'RNA editing and alternative splicing: the importance of co-transcriptional coordination', 'Co-transcriptional splicing of pre-messenger RNAs: considerations for the mechanism of alternative splicing', 'The realization that splicing occurs co-transcriptionally requires two important considerations']
["The consensus view, based on four organisms, is that the majority of splicing events take place co-transcriptionally in most cells and tissues. RNA processing events that take place on the transcribed pre-mRNA include capping, splicing, editing, 3' processing, and polyadenylation. Most of these processes occur co-transcriptionally while the RNA polymerase II (Pol II) enzyme is engaged in transcriptional elongation."]
['yes']