Has the protein GFP been used in transgenesis for live protein imaging?
['we review recent advancement in the functional studies of the three different GnRH neuron systems, mainly focusing on the electrophysiological analysis of the GnRH-green fluorescent protein (GFP) transgenic animals.', 'founders were found to be transgenic for GFP.', 'GFP expression was detected in a wide range of murine tissues', 'Transgenic Xenopus laevis for live imaging in cell and developmental biology.', 'The stable transgenesis of genes encoding functional or spatially localized proteins, fused to fluorescent proteins such as green fluorescent protein (GFP) or red fluorescent protein (RFP), is an extremely important research tool in cell and developmental biology.', 'GFP-transgenic animals for in vivo imaging: rats, rabbits, and pigs.', 'We have further extended the techniques of genetic engineering to rats, rabbits, and pigs, and have created corresponding GFP-transgenic animals.', 'The results revealed that the 3.6-GFP transgenic animals provide a unique model for direct analysis of cellular and molecular mechanisms of pulp repair and tertiary dentinogenesis in vivo.', 'Long-term effects of PERV-specific RNA interference in transgenic pigs.', 'green fluorescent protein (GFP) as reporter of the vector system were consistently expressed in transgenic animals.', 'The ability to specify the expression levels of exogenous genes inserted in the genomes of transgenic animals is critical for the success of a wide variety of experimental manipulations. ', 'Welfare assessment in transgenic pigs expressing green fluorescent protein (GFP).', 'transgenic animals expressing GFP with wildtype animals along various stages of post natal development', 'Production of transgenic chickens expressing a tetracycline-inducible GFP gene.', 'transgenic animals can be readily created to express fluorescently tagged proteins or reporters', 'These findings suggest that mhc2dab:GFP and cd45:DsRed transgenic lines will be instrumental in elucidating the immune response in the zebrafish.', 'f 33 mice born, 28 (81%) carried the transgene DNA and 15 (55.5%) were GFP-positive.', 'Lentiviral vectors containing the green fluorescent protein gene have been successfully used to select transgenic embryos before transfer to a surrogate mother', 'Typically transgenes are generated by placing a promoter upstream of a GFP reporter gene or cDNA of interest, and this often produces a representative expression pattern.', 'Survival and immunogenicity of mesenchymal stem cells from the green fluorescent protein transgenic rat in the adult rat brain.', 'This problem has been lessened by the availability of transgenic animals that express "reporter" genes, such as green fluorescent protein (GFP)', ' full-length GFP fusion proteins was examined, in transgenic animals, ', 'Two stable transgenic lines express GFP prior to hair-bundle formation', 'we generated two transgenic pigs by somatic cell nuclear transfer (SCNT) that express green fluorescent protein (GFP) driven by cytomegalovirus (CMV).', 'Fluorescent proteins such as the green fluorescent protein (GFP) have widely been used in transgenic animals as reporter genes. ', 'Green Fluorescent Protein (GFP) is used extensively as a reporter for transgene expression in Drosophila and other organisms.']
['Yes, the stable transgenesis of genes encoding functional or spatially localized proteins, fused to fluorescent proteins such as green fluorescent protein (GFP) or red fluorescent protein (RFP), is an extremely important research tool in cell and developmental biology.']
['yes']